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Selection of Biocontrol Agent of Tomato Gray Mold Disease from Flower and Pollinator Hive

토마토 꽃과 수정용 벌집으로부터 잿빛곰팡이병 방제 길항균주 선발

  • Kim, Da-Ran (Department of Plant Medicine, Gyeongsang National University) ;
  • Lee, Jun-Taek (Department of Plant Medicine, Gyeongsang National University) ;
  • Kim, Hye sun (Division of Applied Life Science (BK21 Plus), Institute of Agriculture & Life Science, Gyeongsang National University) ;
  • Jeon, Chang Wook (Division of Applied Life Science (BK21 Plus), Institute of Agriculture & Life Science, Gyeongsang National University) ;
  • Kwak, Youn-Sig (Division of Applied Life Science (BK21 Plus), Institute of Agriculture & Life Science, Gyeongsang National University)
  • 김다란 (경상대학교 식물의학과) ;
  • 이준택 (경상대학교 식물의학과) ;
  • 김혜선 (경상대학교 농업생명과학연구원) ;
  • 전창욱 (경상대학교 농업생명과학연구원) ;
  • 곽연식 (경상대학교 농업생명과학연구원)
  • Received : 2017.03.09
  • Accepted : 2017.03.22
  • Published : 2017.03.31

Abstract

Gray mold disease, cause by Botrytis cinerea, occurs severe damage on varieties of fruit and vegetable production, and have no a critical control method. In case of chemicals control, it is a trigger emergence of drug resistance strains due to using them continuously. In addition, the pathogen is difficult to control naturally because it is possible to survive regardless of host status. In this study, microorganisms were isolated from tomato flower and hive samples and in order to select suitable microbial control agents for tomato gray mold disease. During six-months study, we isolated 1,004 isolates from flower and 925 isolates from pollinator hive samples. Among them, 6 strains were selected based on result of antifungal activity test. The selected strains showed not only strong antifungal activity against gray mold pathogen, but also cellulase and protease enzyme activities. The selected strains were identified as Paenibacillus polymyxa. In plant assay, P. polymyxa prevented the gray mold disease occurrence near 75%.

Keywords

Biocontrol;Botrytis cinerea;gray mold;Paenibacillus polymyxa;Tomato

Acknowledgement

Supported by : 농촌진흥청

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