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Establishment of Analytical Method for Pencycuron in Representative Agricultural Commodities by High-Performance Liquid Chromatography

대표 농산물 중 살균제 Pencycuron의 HPLC 정밀 잔류분석법 개발

  • Lee, Hyeri (Geum River Environment Research Center, National Institute of Environmental Research) ;
  • Choi, Hoon (Department of Bio-Environmental Chemistry, Wonkwang University) ;
  • Kim, Byung-Joon (Department of Agricultural Biotechnology, Seoul National University) ;
  • Kim, Eunhye (Department of Agricultural Biotechnology, Seoul National University) ;
  • Kim, Su-Hee (Department of Agricultural Biotechnology, Seoul National University) ;
  • Lee, Jin-Beom (Department of Agricultural Biotechnology, Seoul National University) ;
  • Lee, Young Deuk (Division of Life and Environmental Science, Daegu University) ;
  • Kim, Jeong-Han (Department of Agricultural Biotechnology, Seoul National University)
  • 이혜리 (국립환경과학원 금강물환경연구소) ;
  • 최훈 (원광대학교 생물환경화학과) ;
  • 김병준 (서울대학교 농생명공학부) ;
  • 김은혜 (서울대학교 농생명공학부) ;
  • 김수희 (서울대학교 농생명공학부) ;
  • 이진범 (서울대학교 농생명공학부) ;
  • 이영득 (대구대학교 생명환경학부) ;
  • 김정한 (서울대학교 농생명공학부)
  • Received : 2017.03.07
  • Accepted : 2017.03.21
  • Published : 2017.03.31

Abstract

The single residue analytical method was developed for determining fungicide pencycuron residues in various agricultural commodities with high-performance liquid chromatography (HPLC). Pencycuron residue was extracted with acetone from representative crops such as Korean cabbage, apple, brown rice and green pepper. After ethyl acetate/n-hexane partition and subsequent clean-up with silica gel chromatography, pencycuron residue was quantified by reversed phase HPLC with UV detection at 240 nm. The suspected residue of pencycuron was confirmed using selected-ion monitoring (SIM) LC/mass spectrometry (MS). Instrumental limit of quantitation (ILOQ) and method LOQ (MLOQ) were set at 2 ng and 0.02 mg/kg, respectively. Overall recoveries of pencycuron from different crop samples fortified at three levels (MLOQ, 10MLOQ, 100MLOQ) were 72~108%. This proposed method could be useful as official analytical method for quantification of pencycuron residues in agricultural commodities.

Keywords

Analytical method;HPLC;pencycuron;residue

Acknowledgement

Supported by : 식품의약품안전처

References

  1. Choi, J. C., Y. J. Lee, S. H. Kim, S. Y. Choi, H. J. Choi, S. W. Jeong, H. J. Park and W. S. Kim (2005) Multiresidue analytical method of pesticides in rice by HPLC. J. Environ. Sci. 14(4):445-449.
  2. Fong, W. G, H. A. Moye, J. N. Seiber and J. P. Toth (1999) Pesticide Residues in food: Methods, Technologies, and Regulations. Wiley Interscience. Canada, pp. 3-4, 40-44.
  3. KCPA (Korea Crop Protection Association) (2016) Guideline Crop Protection Agents. Korea Crop Protection Association, Seoul, Korea, pp. 320-322, 1270, 1291.
  4. Kwon, C. H., Y. D. Lee and M. H. Im (2011) Simultaneous determination of orysastrobin and its isomers in rice using HPLC-UV and LC-MS/MS. J. Agri. Food Chem. 59: 10826-10830. https://doi.org/10.1021/jf202228p
  5. Lee, H., M. Riu, H. Park, Y. Na, H. Song, Y. S. Keum, Y. Zhu and J. H. Kim (2009) Establishment of analytical method for fenhexamid residue in korean cabbage, apple, mandarin and green pepper. Korean J. Pestic. Sci. 13(4):223-231.
  6. Lee, Y. D., J. H. Oh and S. W. Jang (2011) High-performance liquid chromatographic determination of phenothrin and silafuofen residues in crops with mass spectrometric confirmation. Korean J. Pestic. Sci. 15(4):389-400.
  7. Lee, H., H. Choi, E. Kim, Y. D. Lee and J. H. Kim (2015) Establishment of analytical method for carpropamid in agricultural commodities using HPLC-DAD/MS. Korean J. Pestic. Sci. 19(3):185-194. https://doi.org/10.7585/kjps.2015.19.3.185
  8. MFDS (Ministry of Food and Drug Safety) (2013) Practical manual for analysis of pesticide residue in food (4th). Ministry of Food and Drug Safety, Cheongju, Korea, pp. 70-71.
  9. MFDS (Ministry of Food and Drug Safety) (2016a) Food code. Ministry of Food and Drug Safety, Cheongju, Korea, pp. 360-362.
  10. MFDS (Ministry of Food and Drug Safety) (2016b) Attached table 3, Pesticides MRLs in Food. In: Food Code. Ministry of Food and Drug Safety, Cheongju, Korea, pp. 69-70.
  11. Miller, J. M. (2005) Chromatography : concepts and contrasts (2nd), Wiley Intersciense, USA, pp. 286-287.
  12. Rolle, S. D. and Cormis, L (1989) High-performance liquid chromatography for the determination of pencycuron residues in several vegetables. J. Agric. Food Chem. 37:975-978. https://doi.org/10.1021/jf00088a033
  13. Rood, D. (2007) The Troubleshooting and Maintenance Guide for Gas Chromatographyers. Wiley-VCH, USA, pp. 16-17, 22.
  14. Turner, J. A (2015) The Pesticide Manual (17th). British Crop Protection Council, Hampshire, UK, pp. 847-848.