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Development of a Residue Analysis Method for Metamifop in Paddy Water, Soil, and Rice with HPLC

HPLC를 이용한 농업용수, 논토양, 및 현미 중 metamifop의 잔류분석법 개발

  • Park, Hee-Woon (Korea Ginseng Corporation Research Institute, Korea Ginseng Corporation) ;
  • Moon, Joon-Kwan (Department of Plant Life and Environmental Sciences, Hankyong National University)
  • 박희원 ((주)한국인삼공사 R&D본부) ;
  • 문준관 (한경대학교 식물생명환경과학과)
  • Received : 2017.03.04
  • Accepted : 2017.03.20
  • Published : 2017.03.31

Abstract

An analytical method for detecting metamifop residue in paddy water, soil, and rice with high performance liquid chromatography (HPLC) was developed. Water was extracted with ethyl acetate before analyzing by HPLC. Soil residues were extracted with acetone under acidic condition and after purifying with $Extrelut^{(R)}$ NT, and silica SPE, the residue was analyzed by HPLC. For residue analysis in rice, the procedure involved extraction with acetone, purification with $Extrelut^{(R)}$ NT, partitioning between acetonitrile/hexane, purification with silica SPE cartridge, and analysis by HPLC. The limit of detection (LOD) was 1.0 ng, limit of quantitation (LOQ) was 3.0 ng, and method limit of quantitation (MLOQ) were 0.001 mg/L for paddy water, 0.01 mg/kg for rice and soil, respectively. Standard calibration curve shows linearity from 0.05 mg/kg to 5.0 mg/kg ($R^2=0.9999$). The recoveries in fortified paddy water were $91.3{\pm}3.5%$ (0.01 mg/L level) and $93.2{\pm}6.3%$ (0.05 mg/L level). The recoveries in fortified paddy soils were $92.5{\pm}4.0%$ (0.1 mg/kg level) and $92.7{\pm}4.0%$ (0.5 mg/kg level) in soil A, while, $102.3{\pm}4.4%$ (0.1 mg/kg level) and $98.9{\pm}7.9%$ (0.5 mg/kg level) in soil B, respectively. The recoveries in fortified rice were $93.0{\pm}6.9%$ (0.1 mg/kg level) and $85.0{\pm}3.5%$ (0.5 mg/kg level). This method was proved to be effective and can be used to determine the metamifop residue in paddy water, paddy soil, and rice.

Keywords

analysis;herbicide;HPLC;metamifop;residue

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