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Comparative analysis of amino acid content of Lentinula edodes, a new variety of shiitake mushroom, in 'Poongnyunko'

표고 신품종 '풍년고'의 아미노산 함량 분석

  • Received : 2017.03.02
  • Accepted : 2017.03.23
  • Published : 2017.03.31

Abstract

In this study, we investigated the amino acids content of shiitake mushrooms grown in eight different media and under three different temperature conditions. The sawdust media were supplemented with 4 kinds of nutrients - rice bran, barley, bean curd refuse, and red ginseng refuse in an 8:2 ratio with or without 0.5% calcium chloride, and the fruiting temperature was set to low ($13-15^{\circ}C$), medium ($18-20^{\circ}C$), or high temperatures ($24-26^{\circ}C$). Seventeen amino acids were detected, and the content varied depending on the compositions of the media. The highest total amino acid content (209.7 mg/g) was measured in mushrooms from the media with red ginseng refuse, and the glutamic acid content was the highest (51.3 mg/g) in mushrooms from the media with barley. In terms of fruiting temperature, the amino acid content was the highest (38.2%) at medium temperature followed by the content at low (32.5%) and high temperature (29.3%). The amino acid composition of shiitake mushrooms was similar between the medium and low temperature conditions.

Keywords

Amino acids;Medium;Poongnyunko;Shiitake;Temperature

References

  1. Breene W. 1990. Nutritional and medicinal value of specialty mushrooms. J Food Prot. 53:883-94. https://doi.org/10.4315/0362-028X-53.10.883
  2. Chang ST, Miles PG. 1989. Edible mushroom and their cultivation CRC press, New York. 120:307-312.
  3. Chang HY, Goo JJ. 2005. Effect of highly activated calcium on the growth of mushrooms. J Mushroom Sci Prod. 3:35-39.(in Korean)
  4. Chang HY, Chung KC, Jang IJ. 2005. Growth and substance transfer effect of Pleurotus cornucopiae by high concentration treatments of selenium(II). J Mushroom Sci Prod. 3:85-89.
  5. Cheung LM, Cheung PCK, Ooi VEC. 2003. Antioxidant activity and total phenolics of edible mushroom extracts. Food Chem. 80:1-7. https://doi.org/10.1016/S0308-8146(02)00227-3
  6. Daniel JS, Steven AC. 1993. Sensitive analysis of cystine/cysteine using 6-aminoquinoquinolyl-N-hydroxysuccinimidy carbamate (AQC) derivatives. Tech Protein Chem. 4:299-306
  7. Eghianruwa Q, Odekanyin O, Kuku A. 2011. Physicochemical properties and acute toxicity studies of a lectin from the saline extract of the fruiting bodies of the shiitake mushroom, Lentinula edodes (Berk). Inter J Biochem Mol Biol. 2:309-317.
  8. Enman J, Rova U, Berglund KA. 2007. Quantification of the bioactive compound eritadenine in selected strains of shiitake mushroom (Lentinus edodes). J Agri Food Chem. 55:1177-1180. https://doi.org/10.1021/jf062559+
  9. Fang H, Luo W, Henkel J, Barbieri J, Green N. 2006. A yeast assay probes the interaction between botulinum neurotoxin serotype B and its SNARE substrate. Natl Acad Sci USA. 103:6958-6963. https://doi.org/10.1073/pnas.0510816103
  10. Hong JS, Kim YH, Kim MK, Sohn HS. 1989. Contents of free amino acids and total amino acids in Agaricus bisporus, Pleurotus ostreatus and Lentinus edodes. Korean J Food Sci Tchnol. 55:466-475.
  11. Jung HS, Park YN, Yoo YB, Jeon DH, Park KM. 2013. Analysis of nutritional contents and physiological activities of Neolentinus lepideus. J Mushroom Sci Prod. 11:261-268. https://doi.org/10.14480/JM.2013.11.4.261
  12. Jang YS, Ryu RR, Kim MK, Ryu SH, Park JH, Park YA, Kim SH, Ka KH, Park WC. 2016. Development of new varieties of Lentinula edodes with commercial values and function. Natl Inst Forest Sci. 101:57-64.
  13. Kalberer PP. 1974. The cultivation of Pleurotus osteratus experiments to elucidate the infulences of different culture condition on the crop yield. Mushroom Sci. 9:653-661.
  14. Kim BK, Shin GG, Jeong BS, Cha JY. 2001. Cholesterol lowering effect of mushrooms power in hyperlipidemic rats. J Korean Soc Food Sci Nutr. 30:510-515.
  15. Kim TH, Jo SH, Kim MJ, Yu YB, Jang MH, Park KM. 2012. Comparative study on nutritional contents of Auricularia spp. J Mushroom Sci Prod. 10:29-36.
  16. Konno S, Tortorelis DG, Fullerton SA, Samadi AA, Hettiarachchi J, Tazaki H. 2001. A possible hypoglycemic effect of maitake mushroom on type 2 diabetic patients. Diabetic Medicine. 18:1010-1011. https://doi.org/10.1046/j.1464-5491.2001.00532-5.x
  17. Koinia SS, Shimono M. 1957. Amino acid fermentation, Production of L-glutamic acid by various microorganism. J Gen Appl Microbial. 3:193-205. https://doi.org/10.2323/jgam.3.193
  18. Lotan R, Berdicesky I, Merzback D, Grossowicz N. 1976. Effect of calcium ions on growth and metabolism of Saccharomyces carlsbergensis. J Gen Microbiol. 92:76-80. https://doi.org/10.1099/00221287-92-1-76
  19. Lee CO, Kim SH, Kang CY, Kim SW, Cho EC, Kim BK. 1981. Studies on the constituents of the higher Fungi of Korea. Kor J Mycol. 9:99-101.(in Korean)
  20. Leem HT. 2012. addition of amino acid according to the sawdust medium Flammulina velutipes fruiting body growth and changes in nutritional ingredients. Konkuk University Degree of Master of Science.
  21. Lee GS, Byun HS, Yoon KH, Lee JS, Choi KC, Jeung EB. 2009. Dietary calcium and vitamin $D_2$ supplementation with enhanced Lentinula edodes improves osteoporosislike symptoms and induces duodenal and renal active calcium transport gene expression in mice. Eur J Nutr. 48:75-83. https://doi.org/10.1007/s00394-008-0763-2
  22. Lee IS, Nishikawa A. 2003. Polyzellus multiplex, a Korean wide mushroom, as a potent chemopreventive agent against stomach cancer. Life Sci. 73:3225-3234. https://doi.org/10.1016/j.lfs.2003.06.006
  23. Park YA, Park WC, Ka KH, Koo CD. 2016. Cultural characteristics and the contents of ${\beta}$-glucan of a new variety of Lentinula edodes, Poongnyunko. Kor J Mycol. 44:296-299.(in Korean)
  24. Ruffio P. 1987. Amino Acid Products and Technology, Business Opportunity Report C-056. Business Communication Co., Inc., Norwalk, Connricut. USA.
  25. Casal S, Oliveria MB, Ferreira MA. 2000. Gas chromatographic quantification of amino acid enantiomers in food matrices by their N(O,S)-ethoxycarbonyl heptafluorobutyl esterderivatives. J Chromatogr A. 866:221-230. https://doi.org/10.1016/S0021-9673(99)01105-X
  26. Yoon KH, Chung SH, Oh HS. 2007. Prediction of shelf life of cold buckwheat noodles mixed with vitamin $D_2$ enriched siitake mushroom and seaweed derived calcium. J Kor Food Sci Nutr. 36:1225-1229. https://doi.org/10.3746/jkfn.2007.36.9.1225
  27. Yoon DY, Park KM, Lee JH. 2010. Characteristics and biological properties of Pleurotus eryngii grown on monosodium glutamate-enriched media. KSBB Journal. 25:277-282.

Acknowledgement

Grant : 품종보호 수입대체용 표고 신품종 개발과 시험포 운영

Supported by : 농림수산식품기술기획평가원(IPET)