Combination effect of acetic acid and citric acid on calcium and phosphorus extraction from shank bone

초산과 구연산의 조합이 사골로부터 칼슘과 인의 용출에 미치는 영향

  • Kim, Dong Chung (Department of Integrated Materials Engineering, Chungwoon University) ;
  • Won, Sun Im (Department of Human Nutrition and Food Science, Chungwoon University) ;
  • In, Man-Jin (Department of Human Nutrition and Food Science, Chungwoon University)
  • Received : 2016.09.30
  • Accepted : 2016.10.24
  • Published : 2017.03.30


This study was conducted to investigate the effect of acetic acid and citric acid treatments on amount and content ratio of calcium (Ca) and phosphorus (P) which were extracted from beef shank bones. The amount of Ca in shank bone extracts significantly increased with increasing citric acid and acetic acid concentration, whereas the amount of P increased with only adding citric acid to extraction media. In the case of combined treatment with 0.2 % acetic acid and 0.1 % citric acid, the amounts of Ca and P increased to 30 and 400 %, respectively and Ca and P ratio were drastically improved from 7.53 to 1.85 compared with treatment of 0.2 % acetic acid. The sensory score of shank bone extract prepared with 0.2 % acetic acid and 0.1 % citric acid treatment showed the highest values in taste and overall acceptability among the tested shank bone extracts.


Supported by : 산학연협력


  1. Cho EK, Wu HJ, Kim BC, Yoo YM, Lee MY (2014) Effect of bone broyh on the quality of cooked rice as home meal replacement products. Food Eng Prog 18: 87-94
  2. Hannig C, Hamkens A, Becker K, Attin R, Attin T (2005) Erosive effects of different acids on bovine enamel: release of calcium and phosphate in vitro. Arch Oral Biol 50: 541-552
  3. Heinonen JK, Lahti RJ (1981) A new and convenient colorimetric determination of inorganic orthophosphate and its application to the assay of inorganic pyrophosphatase. Anal Biochem 113: 313-317
  4. Karanja N, McCarron DA (1986) Calcium and hypertension. Ann Rev Nutr 6: 475-494
  5. Kim BS, Kim GW, Shim JY (2014) Influence of process conditions on the quality characteristics of beef-bone broth. Food Eng Prog 18: 15-19
  6. Kim JH, Cho SH, Seong PN, Hah KH, Yun YK, Lim DG, Park BY, Lee JM, Kim DH, Ahn CN (2008) Effect of different maturity scores and number of extractions on the sensory traits of water extract from Hanwoo shank bones. Korean J Food Sci Ani Resour 28: 45-50
  7. Kim JH, Lee JM, Park BY, Cho SH, Yoo YM, Kim HK, Kim YK (1999) Effect of portion and times of extraction of shsnk bone from Hanwoo bull on physicochemical and sensory characteristics of Komtang. Korean J Food Sci Ani Resour 19: 253-259
  8. Kim MS (2002) The effect on nutrition constituent from beef leg bone by acid condiment. Korean J Soc Food Cookery Sci 18: 349-354
  9. Kim MS (2006) The effect on the nutrition value of beef leg and rib bone soup by boiling time. Korean J Food Culture 21: 161-165
  10. Morgan BR, Artiss JD, Zak B (1993) Calcium determination in serum with stable alkaline arsenazo III and triglyceride clearing. Clin Chem 39: 1608-1612
  11. Park DY, Lee YS (1982) An experiment in extracting efficient nutrients from sagol bone stock. Korean J Nutr Food 11: 47-52
  12. Park DY, Lee YS (1983) The effect of acid and alkali treatment on extracting nutrients from beef bone. Korean J Food Nutr 12: 146-149
  13. Park YH, Park SH, Lee JH, Jo JS (2003) Effects of beef bone extracts on quality of baeck Kimchi. Korean J Soc Food Cookery Sci 19: 188-194
  14. Seol MY, Jang MS (1990) A study on mineral contents in sagol bone stock. Korean J Soc Food Sci 6: 21-26
  15. Wei W, Zhang X, Cui J, Wei Z (2011) Interaction between low molecular weight organic acids and hydroxyapatite with different degrees of crystallinity. Colloids and Surfaces A: Physicochem Eng Aspects 392: 67-75
  16. Yoon GS, Lee BS, Park KH (2009) Effects of the water extract of beef shank bones on the physical and sensory characteristics of cooked rice for Jeonju Bibimbap. J East Asian Soc Dietary Life 19: 1018-1024
  17. Yoshida Y, Van Meerbeek B, Nakayama Y, Yoshioka M, Snauwaert J, Abe Y, Lambrechts P, Vanherle G, Okazaki M (2001) Adhesion to and decalcification of hydroxyapatite by carboxylic acids. J Dental Res 80: 1565-1569