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MiR-186 Inhibited Migration of NSCLC via Targeting cdc42 and Effecting EMT Process

  • Dong, Ying (Department of Radiotherapy, The Tumor Hospital of Jilin Province) ;
  • Jin, Xintian (Department of Thoracic, The Tumor Hospital of Jilin Province) ;
  • Sun, Zhiqiang (Department of Invasive Technology, The Tumor Hospital of Jilin Province) ;
  • Zhao, Yueming (Department of Oncology, The Tumor Hospital of Jilin Province) ;
  • Song, Xianjing (Department of Cardiology, The Second Hospital of Jilin University)
  • Received : 2016.11.29
  • Accepted : 2017.02.08
  • Published : 2017.03.31

Abstract

In this study, qRT-PCR was employed to identify that miR-186 expression level in NSCLC tissues are highly associated with lymph node metastasis. In addition, through the application of western blotting, luciferase assay and qRT-PCR, it was found that miR-186 targeted 3'UTR of cdc42 mRNA and down-regulated cdc42 protein level in a post-transcriptional manner. Transwell assay indicated that cdc42 partially reversed the effect of miR-186 mimics. Besides, miR-186 was proved to regulate EMT by influencing biomarkers of this process and cell adhesion ability. Thus, miR-186 is a potential target for NSCLC therapy. miR-186 is proposed to be one of tumor-suppressors and may serve as a therapeutic target in NSCLC treatment.

Keywords

cdc42;EMT;migration;MiR-186;NSCLC

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