- Volume 30 Issue 2
DOI QR Code
Screening of BCL-2 associated X protein gene polymorphism associated with scrotal hernia in domesticated swine using polymerase chain reaction-restriction fragment length polymorphism
- Manalaysay, Jessica G. (Philippine Carabao Center, Science City of Munoz) ;
- Antonio, Nathaniel D. (College of Science, University of the Philippines Baguio) ;
- Apilado, Ralph Lorenz R. (College of Science, University of the Philippines Baguio) ;
- Bambico, Joseph F. (College of Science, University of the Philippines Baguio) ;
- Mingala, Claro N. (Philippine Carabao Center, Science City of Munoz)
- Received : 2016.01.07
- Accepted : 2016.04.10
- Published : 2017.02.01
Objective: This study was conducted to screen scrotal hernia in domesticated swine from selected breeders in the Philippines. This defect is associated with a cytosine to thymine mutation in the BCL-2 associated X protein (BAX) gene of swine. Methods: Genetic screening was done by DNA extraction followed by amplification and digestion using polymerase chain reaction-restriction fragment length polymorphism, amplifying the 416 bp region of the BAX gene that was subjected to digestion using the Ear I enzyme. Sequencing was also conducted to validate the results. Results: Results revealed that out of 538 samples tested, 411 (76.4%) of the samples were found to be normal whereas the remaining were carriers of the mutation in which 80 (14.9%) were heterozygous mutants and 47 (8.7%) were homozygous mutants. Pietrain breed was found to have the highest incidence. Conclusion: Having a scrotal hernia eliminates the chances of using the boar as a breeder stock because the following generations arising from it would most likely exhibit herniation. It is therefore advised to establish a genetic screening method for Scrotal Hernia in the Philippines to eliminate the negative gene from the herd.
BAX Gene;Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP);Screening;Scrotal Hernia;Swine
- Swine Industry Performance Report as of June 2012 [Internet]. BAS (Bureau of Agricultural Statistics) [cited 2015 October 22]. Available from https://psa.gov.ph/content/swine-industry-performance- report-january-june-2012.
- Vogt DW, Ellersieck MR. Heritability of susceptibility to scrotal herniation in swine. Am J Vet Res 1990;51:1501-3.
- Gatphayak K, Charoensook R, Taesoongnern S, et al. Identification of porcine hernia inguinalis/scrotalis using single nucleotide polymorphism in INSL3 and Bax genes. In: Proceedings of the 2007 Tropentag. 2007 October 9-11: University of Kassel, Witzenhausen, Germany: Cuvillier Verlag Gottingen; 2007. 554 p.
- Bates, RO. Hernias in growing swine. Mich State Univ Pork Q 2008;13:10.
- Beck J, Bornemann-Kolatzki K, Knorr C, Taeubert H, Brenig B. Molecular characterization and exclusion of porcine GUSB as a candidate gene for congenital hernia inguinalis/scrotalis. BMC Vet Res 2006;2:14. https://doi.org/10.1186/1746-6148-2-14
- Du ZQ, Zhao X, Vukasinovic N, et al. Association and haplotype analyses of positional candidate genes in five genomic regions linked to scrotal hernia in commercial swine lines. PLoSONE 2009;4:e4837. https://doi.org/10.1371/journal.pone.0004837
- Zhao X. A candidate gene association study of cryptorchidism and scrotal hernia using canine and porcine models. [MS thesis] Ames, IA: Iowa State University; 2009.
- Deeb N, Cleveland MA, Forni S, et al. Genome-wide association study for scrotal hernia in commercial swine populations. In: Proceedings of the 9th World Congress on Genetics Applied to Livestock Production. 2010 August 1-6: Congress Center Leipzig Messeallee 1, Leipzig, Germany: International Committee for World Congresses on Genetics Applied to Livestock Production and German Society for Animal Science; 2010. 575 p.
- Grindflek E, Moe M, Taubert H, et al. Genome-wide linkage analysis of inguinal hernia in pigs using affected sib pairs. BMC Genet 2006;7:25.
- Ding NS, Mao HR, Guo YM, et al. A genome-wide scan reveals candidate susceptibility loci for swine hernias in an intercross between White Duroc and Erhualian. J AnimSci 2009;87:2469-74.
- Tanyel FC. Obliteration of processus vaginalis: aberrations in the regulatory mechanism result in an inguinal hernia, hydrocele or undescended testis. Turk J Pediatr 2004;46:18-27.
- Osorio LM, De Santiago A, Aguilar-Santelises HM, Mellstedt K, Jondal M. CD6 ligation modulates the Bcl-2/Bax ratio and protects chronic lymphocytic leukemia B cells from apoptosis induced by anti-IgM. Blood 1997;89:2833-41.
- Berge S. Three hereditary anomalies in swine. Hereditas 1941;27: 176-92.
- Rasmussen HB [Internet]. Intechopen; c2012 [cited 2015 September 2]. Available from: http://www.intechopen.com/books/ gel-electrophoresis-principles-and-basics/restriction-fragmentlength- polymorphism-analysis-of-pcr-amplified-fragments-pcrrflp- and-related-te
- Prezioso, V, Jahns, A, Eppendorf Scientific Inc. [Internet]. Drug Discovery Online; c2012 [cited 2015 October 10]. Available from http://www.drugdiscoveryonline.com/doc/using-gradient-pcrto-determine-the-optimum-a-0002
- Vanags DM, Orrenius S, Aguilar‐Santelises M. Alterations in Bcl‐2/ Bax protein levels in platelets form part of an ionomycin‐induced process that resembles apoptosis. Br J Haematol 1997;99:824-31. https://doi.org/10.1046/j.1365-2141.1997.4813284.x
- Yang E, Korsmeyer SJ. Molecular thanatopsis: a discourse on the BCL2 family and cell death. Blood 1996;88:386-401.
- Cory S, Adams JM. The Bcl2 family: regulators of the cellular life-or- death switch. Nat Rev Cancer 2002;2:647-56. https://doi.org/10.1038/nrc883
- Magee WT. Inheritance of scrotal hernia in swine. J Anim Sci 1951;10:516-22. https://doi.org/10.2527/jas1951.102516x