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In vitro stability evaluation of coated lipase

  • Liu, Lu Jie (National Engineering Laboratory of Bio-Feed Safety and Pollution Prevention, Key Laboratory of Animal Nutrition and Feed Science in East China, Ministry of Agriculture, College of Animal Science, Zhejiang University) ;
  • Zhu, Jia (National Engineering Laboratory of Bio-Feed Safety and Pollution Prevention, Key Laboratory of Animal Nutrition and Feed Science in East China, Ministry of Agriculture, College of Animal Science, Zhejiang University) ;
  • Wang, Bin (National Engineering Laboratory of Bio-Feed Safety and Pollution Prevention, Key Laboratory of Animal Nutrition and Feed Science in East China, Ministry of Agriculture, College of Animal Science, Zhejiang University) ;
  • Cheng, Chu (National Engineering Laboratory of Bio-Feed Safety and Pollution Prevention, Key Laboratory of Animal Nutrition and Feed Science in East China, Ministry of Agriculture, College of Animal Science, Zhejiang University) ;
  • Du, Yong Jie (National Engineering Laboratory of Bio-Feed Safety and Pollution Prevention, Key Laboratory of Animal Nutrition and Feed Science in East China, Ministry of Agriculture, College of Animal Science, Zhejiang University) ;
  • Wang, Min Qi (National Engineering Laboratory of Bio-Feed Safety and Pollution Prevention, Key Laboratory of Animal Nutrition and Feed Science in East China, Ministry of Agriculture, College of Animal Science, Zhejiang University)
  • Received : 2016.05.10
  • Accepted : 2016.08.08
  • Published : 2017.02.01

Abstract

Objective: The study was conducted to evaluate the stability of commercial coated lipase (CT-LIP) in vitro. Methods: The capsules were tested under different conditions with a range of temperature, pH, dry heat treatment and steaming treatment, simulated gastric fluid (SGF) and simulated intestinal fluid (SIF) in this work, respectively. Free lipase (uncoated lipase, UC-LIP) was the control group. Lipase relative activities measured in various treatments were used as a reference frame to characterize the stability. Results: The lipase activities were decreased with increasing temperatures (p<0.05), and there was a markedly decline (p<0.01) in lipase comparative activities of UC-LIP at $80^{\circ}C$ compared with CT-LIP group. Higher relative activities of lipase were observed in CT-LIP group compared with the free one under acidic ambient (pH 3 to 7) and an alkaline medium (pH 8 to 12). Residual lipase activities of CT-LIP group were increased (p<0.05) by 5.67% and 35.60% in dry heat and hydrothermal treatments, respectively. The lipase relative activity profile of CT-LIP was raised at first and dropped subsequently (p<0.05) compared with constantly reduced tendency of UC-LIP exposed to both SGF and SIF. Conclusion: The results suggest that the CT-LIP possesses relatively higher stability in comparison with the UC-LIP in vitro. The CT-LIP could retain the potential property to provide sustained release of lipase and thus improved its bioavailability in the gastrointestinal tract.

Keywords

Coated Lipase;Lipase;Stability;Relative Activity;In vitro

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