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Effect of inclusion of different levels of Leucaena silage on rumen microbial population and microbial protein synthesis in dairy steers fed on rice straw

  • Nguyen, Thien Truong Giang (Tropical Feed Resources Research and Development Center (TROFREC), Department of Animal Science, Faculty of Agriculture, Khon Kaen University) ;
  • Wanapat, Metha (Tropical Feed Resources Research and Development Center (TROFREC), Department of Animal Science, Faculty of Agriculture, Khon Kaen University) ;
  • Phesatcha, Kampanat (Tropical Feed Resources Research and Development Center (TROFREC), Department of Animal Science, Faculty of Agriculture, Khon Kaen University) ;
  • Kang, Sungchhang (Agricultural Unit, Department of Education, National Institute of Education)
  • 투고 : 2015.11.20
  • 심사 : 2016.04.10
  • 발행 : 2017.02.01

초록

Objective: Leucaena leucocephala (Leucaena) is a perennial tropical legume that can be directly grazed or harvested and offered to ruminants as hay, silage, or fresh. However, Leucaena contain phenolic compounds, which are considered anti-nutritional factors as these may reduce intake, digestibility and thus animal performance. Therefore, the objective of this experiment was to determine effects of Leucaena silage (LS) feeding levels on rumen microbial populations, N-balance and microbial protein synthesis in dairy steers. Methods: Four, rumen fistulated dairy steers with initial weight of $167{\pm}12kg$ were randomly assigned to receive dietary treatments according to a $4{\times}4$ Latin square design. Treatments were as followings: T1 = untreated rice straw (RS; Control), T2 = 70% RS+30% LS, T3 = 40% RS+60% LS, and T4 = 100% LS. Dairy steers were fed rice straw and LS ad libitum and supplemented with concentrate at 0.2% of body weight/d. Results: Results revealed that the rumen microbial population, especially cellulolytic, proteolytic bacteria and fungal zoospores were enhanced in steers that received 60% of LS (p<0.05), whereas the amylolytic bacteria population was not affected by treatments (p>0.05). Protozoal population was linearly decreased with increasing level of LS (p<0.05). Moreover, N-balance and microbial protein synthesis were enhanced by LS feeding (p<0.05) and were the highest in 60% LS group. Conclusion: Based on this study, it could be concluded that replacement of RS with 60% LS significantly improved microbial population and microbial protein synthesis in diary steers.

키워드

Dairy Steers;Leucaena Silage;Microbial Population;Microbial Protein Synthesis;Rice Straw

과제정보

연구 과제 주관 기관 : Khon Kaen university, Vietnam International Education Department

참고문헌

  1. Wanapat M, Kang S, Polyorach S. Development of feeding systems and strategies of supplementation to enhance rumen fermentation and ruminant production in the tropics. J Anim Sci Biotechnol 2013;4:32. https://doi.org/10.1186/2049-1891-4-32
  2. Wanapat M. Potential uses of local feed resources for ruminants. Trop Amin Health Prod 2009;41:1035-49. https://doi.org/10.1007/s11250-008-9270-y
  3. Aregheore EM. Nutritive and anti-nutritive value of some tree legumes used in ruminant livestock nutrition in Pacific Island countries. J South Pacific Agric 1999;6:50-61.
  4. Barros-Rodríguez M, Solorio-Sánchez J, Sandoval-Castro C, et al. Effects of two intake levels of Leucaena leucocephala on rumen function of sheep. Trop Grassl Forrajes Tropicales 2013;1:55-7. https://doi.org/10.17138/TGFT(1)55-57
  5. AOAC (Association of Official Analytical Chemists). Official methods of Analysis, 16th edn. Animal Feed: Association of Official Analytical Chemists, VA: AOAC International; 1995.
  6. Goering HK, Van Soest PJ. Forage fiber analysis (apparatus, reagent, procedures and some application). Agric. Handbook No. 379, ARS, Washington DC: USDA; 1970.
  7. Burns RE. Method for estimation of tannin in grain sorghum. Agron J 1971;63:511-2. https://doi.org/10.2134/agronj1971.00021962006300030050x
  8. Wanapat M, Poungchompu O. Method for estimation of tannin by Vanillin-HCL method [a modified method of Burns, 1971]. Manual of laboratory of ruminant nutrition. Department of Animal Science, Khon Kaen University, Khon Kaen, Thailand; 2001.
  9. Chen XB, Kyle DJ, Orskov ER. Measurement of allantoin in urine and plasma by high-performance liquid chromatography with pre-column derivatization. J Chromatogr 1993;617:241-7. https://doi.org/10.1016/0378-4347(93)80494-O
  10. Chen XB, Gomes MJ. Estimation of microbial protein supply to sheep and cattle based on urinary excretion of purine derivativesan overview of the technical details. Occassional publication 1992. International Feed Resources Unit, Aberdeen, UK: Rowett Research Institute; 1995.
  11. Galyean ML. Laboratory procedure in animal nutrition research. Las Cruces, NM: New Mexico State University; 1989. pp. 107-22.
  12. Hungate RE. Chapter IV A roll tube method for cultivation of strict anaerobes. Methods Microbiol 1969; 3:117-32.
  13. SAS (Statistical Analysis System) Institute Inc. Guide for Personal Computers. 6th edn. Cary, NC: SAS Institute Inc.; 1998.
  14. Steel RGD, Torrie JH. Principles and procedures of statistic: a biometrical approach. 2nd edn. NY: McGraw-Hill Inc.; 1980.
  15. Hung LV, Wanapat M, Cherdthong A. Effects of leucaena leaf pellet on bacterial diversity and microbial protein synthesis in swamp buffalo fed on rice straw. Livest Sci 2013;151:188-97. https://doi.org/10.1016/j.livsci.2012.11.011
  16. Makkar HP, Blümmel M, Becker K. In vitro effects of and interactions between tannins and saponins and fate of tannins in the rumen. J Sci Food Agric 1995;69:481-93. https://doi.org/10.1002/jsfa.2740690413
  17. Guo YQ, Liu JX, Lu Y, et al. Effect of tea saponin on methanogenesis, microbial community structure and expression of mcrA gene, in cultures of rumen micro‐organisms. Lett Appl Microbiol 2008; 47:421-6. https://doi.org/10.1111/j.1472-765X.2008.02459.x
  18. Newbold CJ, Hillman K. The effect of ciliate protozoa on the turnover of bacterial and fungal protein in the rumen of sheep. Lett Appl Microbiol 1990;11:100-2. https://doi.org/10.1111/j.1472-765X.1990.tb01286.x
  19. Chanjula P, Wanapat M, Wachirapakorn C, Rowlinson P. Effects of various levels of cassava hay on rumen ecology and digestibility in swamp buffaloes. Asian-Australas J Anim Sci 2004;17:663-9. https://doi.org/10.5713/ajas.2004.663
  20. Tagari H, Henis Y, Tamir M, Volcani R. Effect of carob pod extract on cellulolysis, proteolysis, deamination, and protein biosynthesis in an artificial rumen. Appl Environ Microbiol 1965;13:437-42.
  21. Smith AH, Zoetendal E, Mackie RI. Bacterial mechanisms to overcome inhibitory effects of dietary tannins. Microb Ecol 2005; 50:197-205. https://doi.org/10.1007/s00248-004-0180-x
  22. Longo C, Nozella EF, Cabral Filho SLS, Lavorenti N, Vitti DMSS, Abdalla AL. Voluntary intake, apparent digestibility and nitrogen balance by sheep supplemented with Leucaena leucocephala. Livest Res Rur Dev 2008;20:Article #184.
  23. Barahona R, Lascano CE, Cochran R, Morrill J, Titgemeyer EC. Intake, digestion, and nitrogen utilization by sheep fed tropical legumes with contrasting tannin concentration and astringency. J Anim Sci 1997;75:1633-40. https://doi.org/10.2527/1997.7561633x
  24. McNeill DM, Osborne N, Komolong MK, Nankervis D. Condensed tannins in the genus Leucaena and their nutritional significance for ruminants. In: Proceedings of the Leucaena-Adaptation, Quality and Farming Systems, Hanoi: 1998. pp. 205-214.
  25. Pimpa O, Ruengsuwan S, Pimpa B. Protein requirements for maintenance and growing of Thai southern native cattle: practical feeding trial on farm of South-Thailand. In: Oshio E, Otusuka M, Sommart K. editors. Establishment of a feeding standard of beef cattle and a feed database for the indochiness peninsula. Ibaraki, Japan: JIRCAS, Tsukuba; 2009. pp. 86-89.
  26. Javaid A, Nisa M, Sarwar M, Shahzad MA. Ruminal characteristics, blood pH, blood urea nitrogen and nitrogen balance in Nili-ravi Buffalo (Bubalus bubalis) bulls fed diets containing various levels of ruminally degradable protein. Asian-Australas J. Anim Sci 2008; 21:51-8. https://doi.org/10.5713/ajas.2008.70025
  27. Mcdonald P, Edwards RA, Greenhalgh JED, Morgan CA. Animal nutrition. 5th edn. Upper Saddle River, NJ: Prentice Hall; 1995.
  28. Howard H, O'Kiely P, Moloney AP, O'Mara FP. Intake, digestibility, N metabolism and growth in growing steers offered zero-grazed grass supplemented with sucrose. Tullamore, Ireland: Agricultural Research Forum, 2007: p. 12-13.
  29. Stern MD, Hoover WH. Methods for determining and factors affecting rumen microbial protein synthesis: a review. J Anim Sci 1979; 49:1590-603. https://doi.org/10.2527/jas1979.4961590x
  30. Pathak AK. Various factors affecting microbial protein synthesis in the rumen. Vet World 2008;1:186-9.
  31. Waghorn GC. Beneficial and detrimental effects of dietary condensed tannins for sustainable sheep and goat production- Progress and challenges. Anim Feed Sci Technol 2008;147:116-39. https://doi.org/10.1016/j.anifeedsci.2007.09.013