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Effect of pre-miRNA-1658 gene polymorphism on chicken growth and carcass traits

  • Shi, Jianzhou (Institute of Agricultural and Engineering, Nanyang Normal University) ;
  • Sun, Guirong (College of Livestock Husbandry and Veterinary Engineering, Henan Agricultural University)
  • Received : 2016.04.21
  • Accepted : 2016.08.04
  • Published : 2017.04.01

Abstract

Objective: Polymorphisms occurring in the precursor region of microRNAs (miRNAs) affect the target gene and alter the biogenesis of miRNAs, resulting in phenotypic variation. The purpose of the study was to investigate the genetic effects of rs16681031 (C>G) mutation in the precursor region of gga-miR-1658 on the economic traits of the Gushi-Anka chicken F2 resource population. Methods: To explore the effect of miR-1658 polymorphisms on chicken economic traits, the SNP was genotyped by MassArray matrix-assisted laser desorption/ionization-time of flight mass spectrometry. The association between the SNP and chicken body size, growth and carcass traits was determined by linear mixed models. Results: The SNP was not only significantly associated with body weight at the age of 6, 8, 10, 12 weeks, respectively, but also with the breadth of the chicken chest, body slanting length and pelvic breadth at 4 weeks, chest depth at 8 weeks of age, and body slanting length at 12 weeks (p<0.05), respectively. Conclusion: Our data serve as a useful resource for further analysis of miRNA function, and represent a molecular genetic basis for poultry breeding.

Keywords

Carcass Traits;Growth Traits;MassArray Analysis;Polymorphism;Pre-miRNA-1658

Acknowledgement

Supported by : National Natural Science Foundation of China, Nanyang Normal University

References

  1. Aigner A. MicroRNAs (miRNAs) in cancer invasion and metastasis: therapeutic approaches based on metastasis-related miRNAs. J Mol Med (Berl) 2011;89:445-57. https://doi.org/10.1007/s00109-010-0716-0
  2. Li H, Sun GR, Lv SJ, et al. Association study of polymorphisms inside the miR-1657 seed region with chicken growth and meat traits. Br Poult Sci 2012;53:770-6. https://doi.org/10.1080/00071668.2012.750716
  3. Sand M, Gambichler T, Sand D, et al. MicroRNAs and the skin: tiny players in the body's largest organ. J Dermatol Sci 2009;53:169-75. https://doi.org/10.1016/j.jdermsci.2008.10.004
  4. Shi XE, Li YF, Jia L, et al. MicroRNA-199a-5p affects porcine preadipocyte proliferation and differentiation. Int J Mol Sci 2014;15:8526-38. https://doi.org/10.3390/ijms15058526
  5. Zhang J, Zhao H, Gao Y, Zhang W. Secretory miRNAs as novel cancer biomarkers. Biochim Biophys Acta 2012;1826:32-43.
  6. Fiedler SD, Carletti MZ, Hong X, Christenson LK. Hormonal regulation of MicroRNA expression in periovulatory mouse mural granulosa cells. Biol Reprod 2008;79:1030-7. https://doi.org/10.1095/biolreprod.108.069690
  7. Mahdipour M, van Tol HT, Stout TA, Roelen BA. Validating reference microRNAs for normalizing qRT-PCR data in bovine oocytes and preimplantation embryos. BMC Dev Biol 2015;15:25. https://doi.org/10.1186/s12861-015-0075-8
  8. Chen Y, Verfaillie CM. MicroRNAs: the fine modulators of liver development and function. Liver Int 2014;34:976-90. https://doi.org/10.1111/liv.12496
  9. Kim VN, Han J, Siomi MC. Biogenesis of small RNAs in animals. Nat Rev Mol Cell Biol 2009;10:126-39.
  10. Li H, Wang S, Yan F et al. Effect of polymorphism within miRNA-1606 gene on growth and carcass traits in chicken. Gene 2015;566:8-12. https://doi.org/10.1016/j.gene.2015.03.037
  11. Wang X, Gu Z, Jiang H. MicroRNAs in farm animals. Animal 2013;7:1567-75. https://doi.org/10.1017/S1751731113001183
  12. Lazare SS, Wojtowicz EE, Bystrykh LV, de Haan G. microRNAs in hematopoiesis. Exp Cell Res 2014;329:234-8. https://doi.org/10.1016/j.yexcr.2014.08.033
  13. Lin S, Li H, Mu H, et al. Let-7b regulates the expression of the growth hormone receptor gene in deletion-type dwarf chickens. BMC Genomics 2012;13:306. https://doi.org/10.1186/1471-2164-13-306
  14. Wang H, Xiao S, Wang M, et al. In silico identification of conserved microRNAs and their targets in bovine fat tissue. Gene 2015;559:119-28. https://doi.org/10.1016/j.gene.2015.01.021
  15. Luo W, Nie Q, Zhang X. MicroRNAs involved in skeletal muscle differentiation. J Genet Genomics 2013;40:107-16. https://doi.org/10.1016/j.jgg.2013.02.002
  16. Ambros V. The function of animal MicroRNAs. Nature 2004;431:350-5. https://doi.org/10.1038/nature02871
  17. An X, Hou J, Gao T, et al. Single-nucleotide polymorphisms g.151435C>T and g.173057T>C in PRLR gene regulated by bta-miR-302a are associated with litter size in goats. Theriogenology 2015;83:1477-83. https://doi.org/10.1016/j.theriogenology.2015.01.030
  18. Lei B, Gao S, Luo LF, et al. A SNP in the miR-27a gene is associated with litter size in pigs. Mol Biol Rep 2011;38:3725-9. https://doi.org/10.1007/s11033-010-0487-2
  19. Hong JS, Noh SH, Lee JS, et al. Effects of polymorphisms in the porcine microRNA miR-1 locus on muscle fiber type composition and miR-1 expression. Gene 2012;506:211-6. https://doi.org/10.1016/j.gene.2012.06.050
  20. Bartz M, Koscianska E, Szczerbal I, et al. Polymorphism of the porcine miR-30d is associated with adipose tissue accumulation, its fatty acid profile and the ME1 gene expression. Livest Sci 2015;182:54-7. https://doi.org/10.1016/j.livsci.2015.10.019
  21. Wang X, Yu J, Zhang Y, Gong D, Gu Z. Identification and characterization of microRNA from chicken adipose tissue and skeletal muscle. Poult Sci 2012;91:139-49. https://doi.org/10.3382/ps.2011-01656
  22. Bannister SC, Tizard ML, Doran TJ, Sinclair AH, Smith CA. Sexually dimorphic microRNA expression during chicken embryonic gonadal development. Biol Reprod 2009;81:165-76.
  23. Zhang N, Huo Q, Wang X, et al. A genetic variant in pre-miR-27a is associated with a reduced breast cancer risk in younger Chinese population. Gene 2013;529:125-30. https://doi.org/10.1016/j.gene.2013.07.041
  24. Xiong XD, Cho M, Cai XP, et al. A common variant in pre-miR-146 is associated with coronary artery disease risk and its mature miRNA expression. Mutat Res 2014;761:15-20.
  25. Han R, Wei Y, Kang X, et al. Novel SNPs in the PRDM16 gene and their associations with performance traits in chickens. Mol Biol Rep 2012;39:3153-60. https://doi.org/10.1007/s11033-011-1081-y
  26. Han RL, Li ZJ, Li MJ, et al. Novel 9-bp indel in visfatin gene and its associations with chicken growth. Br Poult Sci 2011;52:52-7. https://doi.org/10.1080/00071668.2010.537310
  27. Sambrook J. Russell DW. Molecular cloning: a laboratory manual (3rd Edition). New York: Cold Spring Harbor Laboratory Press; 2001.
  28. Oeth P, Beaulieu M, Park C, et al. iPLEX TM assay: increased plexing efficiency and flexibility for MassARRAY$^{(R)}$ system through single base primer extension with mass-modified terminators. SEQUENOM$^{(R)}$ Application Note; 2005.
  29. Zuker M. Mfold web server for nucleic acid folding and hybridization prediction. Nucleic Acids Res 2003; 31:3406-15. https://doi.org/10.1093/nar/gkg595
  30. Jazdzewski K, Murray EL, Franssila K, et al. Common SNP in pre-miR-146a decreases mature miR expression and predisposes to papillary thyroid carcinoma. Proc Natl Acad Sci USA 2008;105:7269-74. https://doi.org/10.1073/pnas.0802682105
  31. Zhang J, Ying ZZ, Tang ZL, Long LQ, Li K. MicroRNA-148a promotes myogenic differentiation by targeting the ROCK1 gene. J Biol Chem 2012;287:21093-101. https://doi.org/10.1074/jbc.M111.330381
  32. Cai H, Lan X, Li A, et al. SNPs of bovine HGF gene and their association with growth traits in Nanyang cattle. Res Vet Sci 2013;95:483-8. https://doi.org/10.1016/j.rvsc.2013.04.007
  33. Yu Y, Pang Y, Zhao H, et al. Association of a missense mutation in the luteinizing hormone/choriogonadotropin receptor gene (LHCGR) with superovulation traits in Chinese Holstein heifers. J Anim Sci Biotechnol 2012;3:35. https://doi.org/10.1186/2049-1891-3-35
  34. Georges M, Coppieters W, Charlier C. Polymorphic miRNA-mediated gene regulation: contribution to phenotypic variation and disease. Curr Opin Genet Dev 2007;17:166-76. https://doi.org/10.1016/j.gde.2007.04.005
  35. Zorc M, Skok DJ, Godnic I, et al. Catalog of MicroRNA seed polymorphisms in vertebrates. PLOS ONE 2012;7: e30737. https://doi.org/10.1371/journal.pone.0030737
  36. Jevsinek S, Godnic I, Zorc M, et al. Genome-wide in silico screening for microRNA genetic variability in livestock species. Anim Genet 2013;44:669-77. https://doi.org/10.1111/age.12072
  37. Geng L, Zhang C, Li Y, et al. The chicken GGA-Mir-1658* Gene: seed region polymorphisms, frequency distribution and putative targets. J Anim Vet Adv 2011;10:1187-93. https://doi.org/10.3923/javaa.2011.1187.1193
  38. Geng L, Zhang C, Zhao S, et al. Polymorphism of pre-microRNA-1658 gene in chicken. Sci Agric Sin 2015;48:3919-30.