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Detection of Escherichia coli Using Flow Cytometry

유세포 분석기를 이용한 대장균 검출에 관한 연구

  • Kim, Ji-Hye (Department of Environmental Science Keimyung University) ;
  • Park, Sang-Won (Center for Green & Fusion Technologies, Keimyung University) ;
  • Cho, Young Sik (College of Pharmacy, Keimyung University)
  • 김지혜 (계명대학교 환경과학과) ;
  • 박상원 (계명대학교 녹색융합기술연구소) ;
  • 조영식 (계명대학교 약학대학 약학과)
  • Received : 2016.04.22
  • Accepted : 2016.01.02
  • Published : 2017.01.31

Abstract

In this study, bacterial growth was assessed by flow cytometry analysis of fluorescent probes-stained bacteria. Flow cytometry has many advantages of rapid analytical time, a low standard deviation, and highly sensitive detection of live and Dead E.coli over colony forming assay. When untreated bacteria were stained by using Thiazole Orange (TO) and Propidium Iodide (PI), double staining had a short analytical time as compared with that of single staining while its error rate was similar to that of single staining. Through double staining experiments, it was determined that optimal concentrations for TO and PI staining were 420 nM and $9.6{\mu}M$, respectively.

Keywords

Flow cytometry;Escherichia coli;Thiazole orange;Propidium iodide

References

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