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Antioxidant and Anti-Inflammatory Activities of Crude Extract and Solvent Fractions of Allium hookeri

삼채의 조추출물과 유기용매 분획물에 대한 항산화 및 항염증 효과

  • Lee, Yong-Bum (Jeju Biodiversity Research Institute (JBRI), Jeju Technopark (JTP)) ;
  • Ham, Young-Min (Jeju Biodiversity Research Institute (JBRI), Jeju Technopark (JTP)) ;
  • Yoon, Seon-A (Jeju Biodiversity Research Institute (JBRI), Jeju Technopark (JTP)) ;
  • Oh, Dae-Ju (Jeju Biodiversity Research Institute (JBRI), Jeju Technopark (JTP)) ;
  • Song, Sang-Mok (Jeju Biodiversity Research Institute (JBRI), Jeju Technopark (JTP)) ;
  • Hong, In-Choel (Jeju Biodiversity Research Institute (JBRI), Jeju Technopark (JTP)) ;
  • Lee, Si Taek (Jeju Biodiversity Research Institute (JBRI), Jeju Technopark (JTP)) ;
  • Hyun, Ho Bong (Jeju Biodiversity Research Institute (JBRI), Jeju Technopark (JTP)) ;
  • Kim, Chang-Suk (Jeju Biodiversity Research Institute (JBRI), Jeju Technopark (JTP)) ;
  • Yoon, Weon-Jong (Jeju Biodiversity Research Institute (JBRI), Jeju Technopark (JTP))
  • 이용범 ((재)제주테크노파크 생물종다양성연구소) ;
  • 함영민 ((재)제주테크노파크 생물종다양성연구소) ;
  • 윤선아 ((재)제주테크노파크 생물종다양성연구소) ;
  • 오대주 ((재)제주테크노파크 생물종다양성연구소) ;
  • 송상목 ((재)제주테크노파크 생물종다양성연구소) ;
  • 홍인철 ((재)제주테크노파크 생물종다양성연구소) ;
  • 이시택 ((재)제주테크노파크 생물종다양성연구소) ;
  • 현호봉 ((재)제주테크노파크 생물종다양성연구소) ;
  • 김창숙 ((재)제주테크노파크 생물종다양성연구소) ;
  • 윤원종 ((재)제주테크노파크 생물종다양성연구소)
  • Received : 2016.09.23
  • Accepted : 2016.12.19
  • Published : 2017.01.31

Abstract

This study describes the preliminary evaluation of antioxidant and anti-inflammatory activities of Allium hookeri. A. hookeri was extracted using crude extract and then fractionated sequentially with n-hexane, $CH_2Cl_2$, EtOAc, and n-BuOH. To screen for antioxidant and anti-inflammatory agents effectively, we first examined the inhibitory effect of A. hookeri extracts on production of oxidant stresses (2,2-diphenyl-1-picrylhydrazyl, xanthine oxidase, and superoxide). In addition, we examined the inhibitory effects of A. hookeri on production of pro-inflammatory factors (nitric oxide, prostaglandin $E_2$, inducible nitric oxide synthase, and cyclooxygenase-2) in murine macrophage RAW 264.7 cells stimulated with lipopolysaccharide. Of the sequential solvent fractions of A. hookeri, EtOAc fractions showed decreased production of oxidant stresses, and $CH_2Cl_2$ and EtOAc fractions of A. hookeri inhibited production of pro-inflammatory factors. EtOAc fraction inhibited production of pro-inflammatory cytokines (interleukin-6 and -$1{\beta}$). These results suggest that A. hookeri has significant effects on oxidant stresses and pro-inflammatory factors and is a possible antioxidant and anti-inflammatory therapeutic and preventive material.

본 연구는 삼채의 조추출물과 유기용매 분획물들을 가지고 DPPH 라디칼 소거 활성에 의한 항산화 활성 검색 결과 디클로로메탄($CH_2Cl_2$) 분획물과 에틸아세테이트(EtOAc) 분획물에서 라디칼 소거 활성을 나타냈으며, xanthine oxidase 억제 효과는 DPPH 활성 라디칼 소거 활성에서 제일 뛰어났던 에틸아세테이트 분획물에서, superoxide 소거 활성은 헥산(n-hexane) 분획물에서 활성이 나타났다. RAW 264.7 세포에 lipopolysaccharide로 자극을 주고 삼채 주정 추출물 및 유기용매 분획물들을 처리하여 확인해본 결과, 조추출물과 물 분획물을 제외한 나머지 유기용매 분획물에서 염증유발 인자(NO, $PGE_2$, iNOS, COX-2, IL-6 및 $IL-1{\beta}$) 생성억제 효과가 나타났으며, 그중 디클로로메탄 분획물과 에틸아세테이트 분획물에서 억제 효과를 확인할 수 있었다. 본 실험 결과 삼채 조추출물과 유기용매 분획물에서 항산화 효과 및 염증 유발 인자의 생성 억제 효과가 나타났으며, 이러한 결과 삼채에서 유효성분 추출을 통한 항산화, 항염증 물질의 연구 또는 예방하거나 치료할 수 있는 염증 억제 성분의 분리 및 그 작용기전 연구에 중요한 기초 자료가 될 것이라 생각한다.

Keywords

Acknowledgement

Supported by : 농림수산식품기술기획평가원

References

  1. Lee BH, Choi BW, Chun JH, Yu BS. 1996. Extraction of water soluble antioxidants from seaweeds. J Korean Ind & Eng Chem 7: 1069-1077.
  2. Papa S, Skulachev VP. 1997. Reactive oxygen species, mitochondria, apoptosis and aging. Mol Cell Biochem 174: 305-319. https://doi.org/10.1023/A:1006873518427
  3. Halliwell B, Gutteridge JMC. 1999. Free radicals in biology and medicine. Oxford University Press, New York, NY, USA. p 246-350.
  4. Valko M, Leibfritz D, Moncol J, Cronin MT, Mazur M, Telser J. 2007. Free radicals and antioxidants in normal physiological functions and human disease. Int J Biochem Cell Biol 39: 44-84. https://doi.org/10.1016/j.biocel.2006.07.001
  5. Bae SM, Kim JH, Cho CW, Jeong TJ, Ha JU, Lee SC. 2001. Effect of microwave treatment on the antioxidant activity of rice processed by-products. J Korean Soc Food Sci Nutr 30: 1026-1032.
  6. Halliwell B. 1996. Antioxidants in human health and disease. Annu Rev Nutr 16: 33-50. https://doi.org/10.1146/annurev.nu.16.070196.000341
  7. Morrissey PA, O'Brien NM. 1998. Dietary antioxidants in health and disease. Int Dairy J 8: 463-472. https://doi.org/10.1016/S0958-6946(98)00070-3
  8. Yoon WY, Lee JA, Kim KN, Kim JY, Park SY. 2007. In vitro anti-inflammatory activity of the Artemisia fukudo extracts in murine macrophage RAW 264.7 cells. Korean J Food Sci Technol 39: 464-469.
  9. Storck M, Schilling M, Burkhardt K, Prestel R, Abendroth D, Hammer C. 1994. Production of proinflammatory cytokines and adhesion molecules in ex-vivo xenogeneic kidney perfusion. Transpl Int 7: S647-S649. https://doi.org/10.1111/j.1432-2277.1994.tb01464.x
  10. Stuehr DJ, Cho HJ, Kwon NS, Weise MF, Nathan CF. 1991. Purification and characterization of the cytokine-induced macrophage nitric oxide synthase: an FAD- and FMN-containing flavoprotein. Proc Natl Acad Sci U S A 88: 7773-7777. https://doi.org/10.1073/pnas.88.17.7773
  11. Nishida T, Yabe Y, Fu HY, Hayashi Y, Asahi K, Eguchi H, Tsuji S, Tsujii M, Hayashi N, Kawano S. 2007. Geranylgeranylacetone induces cyclooxygenase-2 expression in cultured rat gastric epithelial cells through $NF-{\kappa}B$. Dig Dis Sci 52: 1890-1896. https://doi.org/10.1007/s10620-006-9661-8
  12. Cheon YP, Mohammad LM, Park CH, Hong JH, Lee GD, Song JC, Kim KS. 2009. Bulnesia sarmienti aqueous extract inhibits inflammation in LPS-stimulated RAW 264.7 cells. J Life Sci 19: 479-485. https://doi.org/10.5352/JLS.2009.19.4.479
  13. Leem HH, Kim EO, Seo MJ, Choi SW. 2011. Anti inflammatory effects of volatile flavor extract from herbal medicinal prescriptions including Cnidium officinale Makino and Angelica gigas Nakai. J Soc Cosmet Sci Korea 37: 199-210.
  14. Hyun MR, Lee YS, Park YH. 2011. Antioxidative activity and flavonoid content of Chrysanthemum zawadskii flowers. Korean J Hort Sci Technol 29: 68-73.
  15. Jang MJ, Rhee SJ, Cho SH, Woo MH, Choi JH. 2006. A study on the antioxidative, anti-inflammatory and anti-thrombogenic effects of Zanthoxylum piperitum DC. extract. J Korean Soc Food Sci Nutr 35: 21-27. https://doi.org/10.3746/jkfn.2006.35.1.021
  16. Ayam VS. 2011. Allium hookeri, Thw. Enum. A lesser known terrestrial perennial herb used as food and its ethnobotanical relevance in Manipur. Afr J Food Agric Nutr Dev 11: 5389-5412.
  17. Kim KH, Kim HJ, Byun MW, Yook HS. 2012. Antioxidant and antimicrobial activities of ethanol extract from six vegetables containing different sulfur compounds. J Korean Soc Food Sci Nutr 41: 577-583. https://doi.org/10.3746/jkfn.2012.41.5.577
  18. Vazquez-Prieto MA, Miatello RM. 2010. Organosulfur compounds and cardiovascular disease. Mol Aspects Med 31: 540-545. https://doi.org/10.1016/j.mam.2010.09.009
  19. Bae GC, Bae DY. 2012. The anti-inflammatory effects of ethanol extract of Allium hookeri cultivated in South Korea. Kor J Herbol 27: 55-61.
  20. Blois M. 1958. Antioxidant determinations by the use of a stable free radical. Nature 181: 1199-1200. https://doi.org/10.1038/1811199a0
  21. Nishikimi M, Appaji N, Yagi K. 1972. The occurrence of superoxide anion in the reaction of reduced phenazine methosulfate and molecular oxygen. Biochem Biophys Res Commun 46: 849-854. https://doi.org/10.1016/S0006-291X(72)80218-3
  22. Fridovich I. 1970. Quantitative aspects of the production of superoxide anion radical by milk xanthine oxidase. J Biol Chem 245: 4053-4057.
  23. Korycka-Dahl M, Richardson T, Hicks CL. 1979. Superoxide dismutase activity in bovine milk serum. J Food Prot 42: 867-871. https://doi.org/10.4315/0362-028X-42.11.867
  24. Cheng ZJ, Kuo SC, Chan SC, Ko FN, Teng CM. 1998. Antioxidant properties of butein isolated from Dalbergia odorifera. Biochim Biophys Acta 1392: 291-299. https://doi.org/10.1016/S0005-2760(98)00043-5
  25. Heo SJ, Yoon WJ, Kim KN, Ahn GN, Kang SM, Kang DH, Affan A, Oh C, Jung WK, Jeon YJ. 2010. Evaluation of anti-inflammatory effect of fucoxanthin isolated from brown algae in lipopolysaccharide-stimulated RAW 264.7 macrophages. Food Chem Toxicol 48: 2045-2051. https://doi.org/10.1016/j.fct.2010.05.003
  26. Ren K, Torres R. 2009. Role of interleukin-$1{\beta}$ during pain and inflammation. Brain Res Rev 60: 57-64. https://doi.org/10.1016/j.brainresrev.2008.12.020
  27. Santos-Gomes PC, Seabra RM, Andrade PB, Fernandes-Ferreira M. 2003. Determination of phenolic antioxidant compounds produced by calli and cell suspensions of sage (Salvia officinalis L.). J Plant Physiol 160: 1025-1032.
  28. Tezuka Y, Irikawa S, Kaneko T, Banskota AH, Nagaoka T, Xiong Q, Hase K, Kadota S. 2001. Screening of Chinese herbal drug extracts for inhibitory activity on nitric oxide production and identification of an active compound of Zanthoxylum bungeanum. J Ethnopharmacol 77: 209-217. https://doi.org/10.1016/S0378-8741(01)00300-2
  29. Masferrer JL, Zweifel BS, Manning PT, Hauser SD, Leahy KM, Smith WG, Isakson PC, Seibert K. 1994. Selective inhibition of inducible cyclooxygenase 2 in vivo is antiinflammatory and nonulcerogenic. Proc Natl Acad Sci U S A 91: 3228-3232. https://doi.org/10.1073/pnas.91.8.3228
  30. Hyun EA, Lee HJ, Yoon WJ, Park SY, Kang HK, Kim SJ, Yoo ES. 2004. Inhibitory effect of Salcia officinalis on the inflammatory cytokines and inducible nitric oxide synthesis in murine macrophage RAW 264.7. Yakhak Hoeji 48: 159-164.
  31. Kim EY, Moudgil KD. 2008. Regulation of autoimmune inflammation by pro-inflammatory cytokines. Immunol Lett 120: 1-5. https://doi.org/10.1016/j.imlet.2008.07.008
  32. Kim JH, Bachmann RA, Chen J. 2009. Interleukin-6 and insulin resistance. Vitam Horm 80: 613-633.