Isolation of Gene according to the Physiological Changes of Lespedeza cuneata. G don by the Convergence Study using a Computer Program and NGS (Next Generation Sequencing)

NGS (Next Generation Sequencing)와 컴퓨터 프로그램의 융합적 연구를 통한 비수리(Lespedeza cuneata. G. don)의 생리적 변화에 따른 유용 유전자 분리

  • Ahn, Chul-Hyun (Korea National College of Agriculture and Fisheries)
  • Received : 2017.10.23
  • Accepted : 2017.12.20
  • Published : 2017.12.28


This study was carried out to investigate the possibility of isolating the useful gene of soybean plant, anthocyanin, through NGS (Next Generation Sequencing) and molecular biology experiments. Lespedeza cuneata. G. don is a resource plant but has many useful materials. Especially, D-pinitol, which has anti-diabetic function, is contained in a large amount. However, the gene related to the biosynthesis of D-piniol has not been isolated in the non-spermatid. Lespedeza cuneata. G. don was treated with abiotic stress (drought), total RNA was extracted, and a library was constructed to perform NGS. In this way, the genes involved in D-pinitol biosynthesis were isolated and sequenced in silico. In order to support this, ononitol epimerase involved in D-pinitol amplification was identified using the Blast program and RT-PCR confirmed the increased gene expression in vitro, and the gene was isolated and identified by convergence study.


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