Treefrog lateral line as a mean of individual identification through visual and software assisted methodologies

  • Kim, Mi Yeon (Department of Life Sciences and Division of EcoScience, Ewha Womans University) ;
  • Borzee, Amael (Department of Life Sciences and Division of EcoScience, Ewha Womans University) ;
  • Kim, Jun Young (Department of Life Sciences and Division of EcoScience, Ewha Womans University) ;
  • Jang, Yikweon (Department of Life Sciences and Division of EcoScience, Ewha Womans University)
  • Received : 2017.06.27
  • Accepted : 2017.12.07
  • Published : 2017.12.31


Background: Ecological research often requires monitoring of a specific individual over an extended period of time. To enable non-invasive re-identification, consistent external marking is required. Treefrogs possess lateral lines for crypticity. While these patterns decrease predator detection, they also are individual specific patterns. In this study, we tested the use of lateral lines in captive and wild populations of Dryophytes japonicus as natural markers for individual identification. For the purpose of the study, the results of visual and software assisted identifications were compared. Results: In normalized laboratory conditions, a visual individual identification method resulted in a 0.00 rate of false-negative identification (RFNI) and a 0.0068 rate of false-positive identification (RFPI), whereas Wild-ID resulted in RFNI = 0.25 and RFNI = 0.00. In the wild, female and male data sets were tested. For both data sets, visual identification resulted in RFNI and RFPI of 0.00, whereas the RFNI was 1.0 and RFPI was 0.00 with Wild-ID. Wild-ID did not perform as well as visual identification methods and had low scores for matching photographs. The matching scores were significantly correlated with the continuity of the type of camera used in the field. Conclusions: We provide clear methodological guidelines for photographic identification of D. japonicus using their lateral lines. We also recommend the use of Wild-ID as a supplemental tool rather the principal identification method when analyzing large datasets.



Supported by : Rural Development Administration


  1. Amstrup SC, McDonald TL, Manly BF, editors. Handbook of capture-recapture analysis. Princeton: Princeton University Press; 2010.
  2. Bendik, N. F., Morrison, T. A., Gluesenkamp, A. G., Sanders, M. S., & O’Donnell, L. J. (2013). Computer-assisted photo identification outperforms visible implant elastomers in an endangered salamander, Eurycea tonkawae. PLoS One, 8(3), e59424.
  3. Bolger, D. T., Morrison, T. A., Vance, B., Lee, D., & Farid, H. (2012). A computerassisted system for photographic mark-recapture analysis. Methods in Ecology and Evolution, 3(5), 813-822.
  4. Choi, N., Jang, Y (2014). Background matching by means of dorsal color change in treefrog populations (Hyla japonica). Journal of Experimental Zoology. Part A, Ecological Genetics and Physiology, 321, 108-118.
  5. Church, D. R., Bailey, L. L., Wilbur, H. M., Kendall, W. L., & Hines, J. E. (2007). Iteroparity in the variable environment of the salamander Ambystoma tigrinum. Ecology, 88, 891-903.
  6. Clarke, R. D (1972). The effect of toe clipping on survival in Fowler's toad (Bufo woodhousei fowleri). Copeia, 1, 182-185.
  7. Correa, D. T. (2013). Population declines: toe-clipping vital to amphibian research. Nature, 493, 305-305.
  8. Dala-Corte, R. B., Moschetta, J. B., & Becker, F. G. (2016). Photo-identification as a technique for recognition of individual fish: a test with the freshwater armored catfish Rineloricaria aequalicuspis Reis & Cardoso, 2001 (Siluriformes: Loricariidae). Neotrop Ichthyol., 14(1), e150074.
  9. Donnelly, M. A., Guyer, C., Juterbock, J. E., & Alford, R. A. (1994). Techniques for marking amphibians. In: Heyer, R., Donnelly, M. A., Foster, M., & Mcdiarmid, R., editors. (2004). Measuring and monitoring biological diversity: Standard methods for amphibians, pp 277-284. Washinton, DC: Smithsonian Institution Press.
  10. Duellman, W. E., Marion, A. B., Hedges, S. B (2016). Phylogenetics, classification, and biogeography of the treefrogs (Amphibia: Anura: Arboranae). Zootaxa, 4104, 1-109.
  11. Dufresnes, C., Litvinchuk, S. N., Borzee, A., Jang, Y., Li, J-T., Miura, I., Perrin, N., Stock, M (2016). Phylogeography reveals an ancient cryptic radiation in East-Asian tree frogs (Hyla japonica group) and complex relationships between continental and island lineages. BMC Evolutionary Biology, 16, 253
  12. Elgue, E., Pereira, G., Achaval-Coppes, F., Maneyro, R (2014). Validity of photoidentification technique to analyze natural markings in Melanophryniscus montevidensis (Anura: Bufonidae). Phyllomedusa: Journal of Herpetology, 13(1), 59-66.
  13. Ferner, J. W. (1979). A review of marking techniques for amphibians and reptiles. Society for the Study of Amphibians and Reptiles, Herpetol Circ., 9, 1-41.
  14. Gamble, L., Ravela, S., & McGarigal, K. (2008). Multi-scale features for identifying individuals in large biological databases: an application of pattern recognition technology to the marbled salamander Ambystoma opacum. Journal of Applied Ecology, 45, 170-180.
  15. Golay, N., Durrer, H (1994). Inflammation due to toe-clipping in natterjack toads (Bufo calamita). Amphibia-Reptilia, 15, 81-83.
  16. Guimaraes, M., Correa, D. T., Filho, S. S., Oliveira, T. A., Doherty, P. F., & Sawaya, R. J. (2014). One step forward: contrasting the effects of toe clipping and PIT tagging on frog survival and recapture probability. Ecology and Evolution, 4, 1480-1490.
  17. Hastings, K. K., Hiby, L. A., & Small, R. J. (2008). Evaluation of a computer-assisted photograph-matching system to monitor naturally marked harbor seals at Tugidak Island, Alaska. Journal of Mammalogy, 89, 1201-1211.
  18. Kang, C., Kim, Y. E., & Jang, Y. (2016). Colour and pattern change against visually heterogeneous backgrounds in the tree frog Hyla japonica. Scientific Reports, 6, 22601.
  19. Kenyon, N., Phillott, A. D., Alford, R. A (2009). Evaluation of the photographic identification method (PIM) as a tool to identify adult Litoria Genimaculata (Anura: Hylidae). Herpetological Conservation and Biology, 4(3), 403-410.
  20. Kim, J.Y. (2015). Lekking behavior in the Japanese treefrog Hyla japonica. Seoul, Republic of Korea: Ewha Womans University, p. 62.
  21. Knox, C. D., Cree, A., Seddon, P. J (2012). Accurate identification of individual geckos (Naultinus gemmeus) through dorsal pattern differentiation. New zeal Journal of Ecology, 37, 60-66.
  22. Lama, F. D., Rocha, M. D., Andrade, M. A., & Nascimento, L. B. (2011). The use of photography to identify individual tree frogs by their natural marks. S am. Journal of Herpetology, 6, 198-204.
  23. Lemckert, F. (1996). Effects of toe-clipping on the survival and behaviour of the Australian frog Crinia signifera. Amphibia-Reptilia, 17, 287-290.
  24. Lukacs, P. M., & Burnham, K. P. (2005). Research notes: estimating population size from DNA-based closed capture-recapture data incorporating genotyping error. Journal of Wildlife Management, 69, 396-403.<0396:EPSFDC>2.0.CO;2
  25. McDonald, D., Dutton, P., Brander, R., Basford, S (1996). Use of pineal spot (pink spot) photographs to identify leatherback turtles. Herpetol Rev, 27, 11-11.
  26. Roh, G., Borzee, A., & Jang, Y. (2014). Spatiotemporal distributions and habitat characteristics of the endangered treefrog, Hyla suweonensis, in relation to sympatric H. japonica. Ecological Informatics, 24, 78-84.
  27. Stuart, S. N., Chanson, J. S., Cox, N. A., Young, B. E., Rodrigues, A. S., Fischman, D. L., & Waller, R. W. (2004). Status and trends of amphibian declines and extinctions worldwide. Science, 306, 1783-1786.
  28. Waddle, J. H., Rice, K. G., Mazzotti, F. J., & Percival, H. F. (2008). Modeling the effect of toe clipping on treefrog survival: beyond the return rate. Journal of Herpetology, 42, 467-473.
  29. Waichman, A. V. (1992). An alphanumeric code for toe clipping amphibians and reptiles. Herpetol Rev., 23(1), 19-21.
  30. Wake, D. B. (2012). Facing extinction in real time. Science, 335, 1052-1053.
  31. Yoshizaki, J., Pollock, K. H., Brownie, C., & Webster, R. A. (2009). Modeling misidentification errors in capture-recapture studies using photographic identification of evolving marks. Ecology, 90, 3-9.

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