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Effects of titanium oxide nanoparticles on Oryzias latipes embryos and sac-fry under different irradiation conditions

  • Nam, Sun-Hwa (Department of Environmental Health Science, Konkuk University) ;
  • Shin, Yu-Jin (Department of Environmental Health Science, Konkuk University) ;
  • An, Youn-Joo (Department of Environmental Health Science, Konkuk University)
  • Received : 2017.05.04
  • Accepted : 2017.06.18
  • Published : 2017.12.31

Abstract

Some phototoxicity of titanium dioxide nanoparticles ($TiO_2$ NPs) has been reported in recent years in studies with fish embryos or larvae. However, it is necessary to focus on the potential effects of embryonic exposure due to irreversible abnormalities and mortalities observed in sac-fry, and to expand various fish embryos to generate multiple test species. The aim of this study was to evaluate the effects of $TiO_2$ NPs under different irradiation conditions in exposed Oryzias latipes (O. latipes) at the embryonic and sac-fry stages. The effects of different irradiation conditions were observed using ultra-violet (UV) and visible light, and the corresponding effects were monitored by determining cumulative mortality and abnormality. O. latipes were exposed for 8 d to 0, 1, 5, 10, or 50 mg/L $TiO_2$ NPs under UV ($4,818.86mW/m^2$ at the bottom of clear vials) or visible light, after which the embryos were transferred to NP-free embryo-rearing solution until 16 days post fertilization (dpf). Abnormalities of embryos and sac-fry increased at high $TiO_2$ NP concentrations under UV irradiation, compared to control samples treated with visible light or UV irradiation alone. This work provides information regarding the phototoxicity of $TiO_2$ NPs using O. latipes at the embryonic and sac-fry stages.

Keywords

Fish embryo;Oryzias latipes;Phototoxicity;$TiO_2$ NPs;UV irradiation

Acknowledgement

Supported by : National Research Foundation of Korea (NRF), Korea Environment Industry & Technology Institute (KEITI)

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