Acute and 13-week subchronic toxicological evaluations of turanose in mice

  • Chung, Joo-Yeon ;
  • Lee, Jihye ;
  • Lee, Daeyeon ;
  • Kim, Eunju ;
  • Shin, Jae-Ho ;
  • Seok, Pu Reum ;
  • Yoo, Sang-Ho ;
  • Kim, Yuri
  • Received : 2017.07.31
  • Accepted : 2017.09.20
  • Published : 2017.12.01


BACKGROUD/OBJECTIVES: Turanose, ${\alpha}$-D-glucosyl-($1{\rightarrow}3$)-${\alpha}$-D-fructose, is a sucrose isomer which naturally exists in honey. To evaluate toxicity of turanose, acute and subchronic oral toxicity studies were conducted with ICR mice. MATERIALS AND METHODS: For the acute oral toxicity study, turanose was administered as a single oral dose [10 g/kg body weight (b.w.)]. In the subchronic toxicity study, ICR mice were administered 0, 1.75, 3.5, and 7 g/kg b.w. doses of turanose daily for 13 weeks. RESULTS: No signs of acute toxicity, including abnormal behavior, adverse effect, or mortality, were observed over the 14-day study period. In addition, no changes in body weight or food consumption were observed and the median lethal dose (LD50) for oral intake of turanose was determined to be greater than 10 g/kg b.w. General clinical behavior, changes in body weight and food consumption, absolute and relative organ weights, and mortality were not affected in any of the treatment group for 13 weeks. These doses also did not affect the macroscopic pathology, histology, hematology, and blood biochemical analysis of the mice examined. CONCLUSION: No toxicity was observed in the acute and 13-week subchronic oral toxicology studies that were conducted with ICR mice. Furthermore, the no-observed-adverse-effect level is greater than 7 g/kg/day for both male and female ICR mice.


Toxicity;turanose;ICR mice;$LD_{50}$;NOAEL


  1. Augustin LS, Franceschi S, Jenkins DJ, Kendall CW, La Vecchia C. Glycemic index in chronic disease: a review. Eur J Clin Nutr 2002;56:1049-71.
  2. Livesey G, Taylor R, Hulshof T, Howlett J. Glycemic response and health--a systematic review and meta-analysis: relations between dietary glycemic properties and health outcomes. Am J Clin Nutr 2008;87:258S-268S.
  3. Holub I, Gostner A, Theis S, Nosek L, Kudlich T, Melcher R, Scheppach W. Novel findings on the metabolic effects of the low glycaemic carbohydrate isomaltulose (Palatinose). Br J Nutr 2010; 103:1730-7.
  4. Malik VS, Schulze MB, Hu FB. Intake of sugar-sweetened beverages and weight gain: a systematic review. Am J Clin Nutr 2006;84: 274-88.
  5. White JW, Hoban N. Composition of honey. IV. Identification of the disaccharides. Arch Biochem Biophys 1959;80:386-92.
  6. Shibuya T, Mandai T, Kubota M, Fukuda S, Kurimoto M, Tsujisaka Y. Production of turanose by cyclomaltodextrin glucanotransferase from Bacillus stearothermophilus. J Appl Glycosci 2004;51:223-7.
  7. Park MO, Lee BH, Lim E, Lim JY, Kim Y, Park CS, Lee HG, Kang HK, Yoo SH. Enzymatic process for high-yield turanose production and its potential property as an adipogenesis regulator. J Agric Food Chem 2016;64:4758-64.
  8. Wang R, Bae JS, Kim JH, Kim BS, Yoon SH, Park CS, Yoo SH. Development of an efficient bioprocess for turanose production by sucrose isomerisation reaction of amylosucrase. Food Chem 2012;132:773-9.
  9. Pikis A, Immel S, Robrish SA, Thompson J. Metabolism of sucrose and its five isomers by Fusobacterium mortiferum. Microbiology 2002;148:843-52.
  10. Thompson J, Robrish SA, Pikis A, Brust A, Lichtenthaler FW. Phosphorylation and metabolism of sucrose and its five linkageisomeric alpha-D-glucosyl-D-fructoses by Klebsiella pneumoniae. Carbohydr Res 2001;331:149-61.
  11. Grenby TH. Advances in Sweeteners. London: Blackie Academic & Professional; 1996.
  12. Dahlqvist A. Characterization of hog intestinal invertase as a glucosido-invertase. III. Specificity of purified invertase. Acta Chem Scand 1960;14:63-71.
  13. Organisation for Economic Co-operation and Development. OECD guideline for testing for chemicals: acute oral toxicity-fixed dose procedure [Internet]. Paris: Organisation for Economic Co-operation and Development; 2016 [cited 2017 May 6]. Available from: oxicity-fixed-dose-procedure_9789264070943-en.
  14. Lina BA, Jonker D, Kozianowski G. Isomaltulose (Palatinose): a review of biological and toxicological studies. Food Chem Toxicol 2002;40:1375-81.
  15. Jonker D, Lina BA, Kozianowski G. 13-Week oral toxicity study with isomaltulose (Palatinose) in rats. Food Chem Toxicol 2002;40:1383-9.
  16. Reagan-Shaw S, Nihal M, Ahmad N. Dose translation from animal to human studies revisited. FASEB J 2008;22:659-61.


Supported by : National Research Foundation of Korea