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Acute and 13-week subchronic toxicological evaluations of turanose in mice

  • Chung, Joo-Yeon ;
  • Lee, Jihye ;
  • Lee, Daeyeon ;
  • Kim, Eunju ;
  • Shin, Jae-Ho ;
  • Seok, Pu Reum ;
  • Yoo, Sang-Ho ;
  • Kim, Yuri
  • Received : 2017.07.31
  • Accepted : 2017.09.20
  • Published : 2017.12.01

Abstract

BACKGROUD/OBJECTIVES: Turanose, ${\alpha}$-D-glucosyl-($1{\rightarrow}3$)-${\alpha}$-D-fructose, is a sucrose isomer which naturally exists in honey. To evaluate toxicity of turanose, acute and subchronic oral toxicity studies were conducted with ICR mice. MATERIALS AND METHODS: For the acute oral toxicity study, turanose was administered as a single oral dose [10 g/kg body weight (b.w.)]. In the subchronic toxicity study, ICR mice were administered 0, 1.75, 3.5, and 7 g/kg b.w. doses of turanose daily for 13 weeks. RESULTS: No signs of acute toxicity, including abnormal behavior, adverse effect, or mortality, were observed over the 14-day study period. In addition, no changes in body weight or food consumption were observed and the median lethal dose (LD50) for oral intake of turanose was determined to be greater than 10 g/kg b.w. General clinical behavior, changes in body weight and food consumption, absolute and relative organ weights, and mortality were not affected in any of the treatment group for 13 weeks. These doses also did not affect the macroscopic pathology, histology, hematology, and blood biochemical analysis of the mice examined. CONCLUSION: No toxicity was observed in the acute and 13-week subchronic oral toxicology studies that were conducted with ICR mice. Furthermore, the no-observed-adverse-effect level is greater than 7 g/kg/day for both male and female ICR mice.

Keywords

Toxicity;turanose;ICR mice;$LD_{50}$;NOAEL

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Acknowledgement

Supported by : National Research Foundation of Korea