Xanthomonas euvesicatoria Causes Bacterial Spot Disease on Pepper Plant in Korea

  • Received : 2016.01.17
  • Accepted : 2016.04.04
  • Published : 2016.10.01


In 2004, bacterial spot-causing xanthomonads (BSX) were reclassified into 4 species-Xanthomonas euvesicatoria, X. vesicatoria, X. perforans, and X. gardneri. Bacterial spot disease on pepper plant in Korea is known to be caused by both X. axonopodis pv. vesicatoria and X. vesicatoria. Here, we reidentified the pathogen causing bacterial spots on pepper plant based on the new classification. Accordingly, 72 pathogenic isolates were obtained from the lesions on pepper plants at 42 different locations. All isolates were negative for pectolytic activity. Five isolates were positive for amylolytic activity. All of the Korean pepper isolates had a 32 kDa-protein unique to X. euvesicatoria and had the same band pattern of the rpoB gene as that of X. euvesicatoria and X. perforans as indicated by PCR-restriction fragment length polymorphism analysis. A phylogenetic tree of 16S rDNA sequences showed that all of the Korean pepper plant isolates fit into the same group as did all the reference strains of X. euvesicatoria and X. perforans. A phylogenetic tree of the nucleotide sequences of 3 housekeeping genes-gapA, gyrB, and lepA showed that all of the Korean pepper plant isolates fit into the same group as did all of the references strains of X. euvesicatoria. Based on the phenotypic and genotypic characteristics, we identified the pathogen as X. euvesicatoria. Neither X. vesicatoria, the known pathogen of pepper bacterial spot, nor X. perforans, the known pathogen of tomato plant, was isolated. Thus, we suggest that the pathogen causing bacterial spot disease of pepper plants in Korea is X. euvesicatoria.


bacterial spot disease;pepper plants;re-identification;Xanthomonas euvesicatoria


  1. Almeida, N. F., Yan, S., Cai, R., Clarke, C. R., Morris, C. E., Schaad, N. W., Schuenzel, E. L., Lacy, G. H., Sun, X., Jones, J. B., Castillo, J. A., Bull, C. T., Leman, S., Guttman, D. S., Setubal, J. C. and Vinatzer, B. A. 2010. PAMDB, a multilocus sequence typing and analysis database and website for plant-associated microbes. Phytopathology 100:208-215.
  2. Bouzar, H., Jones, J. B., Minsavage, G. V., Stall, R. E. and Scott, J. W. 1994. Proteins unique to phenotypically distinct groups of Xanthomonas campestris pv. vesicatoria revealed by silver staining. Phytopathology 84:39-44.
  3. De Ley, J. 1978. Modern molecular methods in bacterial taxonomy: evaluation, application, prospects. In: Proceedings of the 4th International Conference on Plant Pathogenic Recherche Agronomique (INRA), Angers, France.
  4. Doidge, E. M. 1921. A tomato canker. Ann. Appl. Biol. 7:407-430.
  5. Dye, D. W. 1966. Cultural and biochemical reaction of additional Xanthomonas species. N. Z. J. Sci. 9:913-919.
  6. Ferreira-Tonin, M., Rodrigues-Neto, J., Harakava, R. and Destefano, S. A. 2012. Phylogenetic analysis of Xanthomonas based on partial rpoB gene sequences and species differentiation by PCR-RFLP. Int. J. Syst. Evol. Microbiol. 62:1419-1424.
  7. Gardner, M. W. and Kendrick, J. B. 1921. Bacterial spot of tomato. J. Agr. Res. 21:123-156.
  8. Hamza, A. A., Robene-Soustrade, I., Jouen, E., Lefeuvre, P., Chiroleu, F., Fisher-Le Saux, M., Gagnevin, L. and Pruvost, O. 2012. Multilocus sequence analysis-and amplified fragment length polymorphism-based characterization of xanthomonads associated with bacterial spot of tomato and pepper and their relatedness to Xanthomonas species. Syst. Appl. Microbiol. 35:183-190.
  9. Jones, J. B., Bouzar, H., Stall, R. E., Almira, E. C., Robert, P. D., Bowen, B. W., Sudberry, J., Strickler, P. M. and Chun, J. 2000. Systematic analysis of xanthomonads (Xanthomonas spp.) associated with pepper and tomato lesions. Int. J. Syst. Evol. Microbiol. 50:1211-1219.
  10. Jones, J. B., Jones, J. P., Stall, R. E. and Zitter, T. A. 1991. Compendium of tomato diseases. APS Press, St. Paul, MN, USA. p. 73.
  11. Jones, J. B., Lacy, G. H., Bouzar, H., Stall, R. E. and Schaad, N. W. 2004. Reclassification of the xanthomonads associated with bacterial spot disease of tomato and pepper. Syst. Appl. Microbiol. 27:755-762.
  12. Kebede, M., Timilsina, S., Ayalew, A., Admassu, B., Potnis, N., Minsavage, G. V., Goss, E. M., Hong, J. C., Strayer, A., Paret, M., Jones, J. B. and Vallad, G. E. 2014. Molecular characterization of Xanthomonas strains responsible for bacterial spot of tomato in Ethiopia. Eur. J. Plant Pathol. 140:677-688.
  13. Kim, C. H. 2004. Review of disease incidence of major crop in 2003. Res. Plant Dis. 10:1-7.
  14. Lee, S. D. and Cho, Y. S. 1996. Copper resistance and race distribution of Xanthomonas campestris pv. vesicatoria on pepper in Korea. Korean J. Plant Pathol. 12:150-155.
  15. Lee, S. D., Yoon, C. M., Lee, Y. K., Choi, Y. C. and Cho, Y. S. 1999. Occurrence and distribution of bacterial canker of red pepper caused by Clavibacter michiganensis subsp. michiganensis. Plant Dis. Agric. 5:105-110.
  16. Myung, I. S., Hong, S. K., Lee, Y. K., Choi, H. W., Shim, H. S., Park, J. W., Park, K. S., Lee, S. Y., Lee, S. D., Lee, S. H., Choi, H. S., Kim, Y. G., Shin, D. B., Ra, D. S., Yeh, W. H., Han, S. S. and Cho, W. D. 2006. Review of disease incidences of major crops of the South Korea in 2005. Res. Plant Dis. 12:153-157.
  17. Myung, I. S., Moon, S. Y., Jeong, I. H., Lee, Y. K. and Ra, D. S. 2009. Bacterial spot of tomato caused by Xanthomonas perforans, a new disease in Korea. Plant Dis. 93:1349.
  18. Myung, I. S., Park, K. S., Hong, S. K., Park, J. W., Shim, H. S., Lee, Y. K., Lee, S. Y., Lee, S. D., Lee, S. H., Choi, H. W., Heu, S. G., Shin, D. B., Ra, D. S., Yeh, W. H. and Cho, W. D. 2005. Review of disease incidence of major crops of the South Korea in 2004. Res. Plant Dis. 11:89-92.
  19. Myung, I. S., Yoon, M. J., Lee, J. Y., Kim, Y. S., Kwon, J. H., Lee, Y. K. and Shim, H. S. 2015. Bacterial spot of hot pepper, caused by Xanthomonas euvesicatoria, a new disease in Korea. Plant Dis. 99:1640.
  20. Obradovic, A., Mavridis, A., Rudolph, K., Janse, J. D., Arsenijevic, M., Jones, J. B., Minsavage, G. V. and Wang, J. F. 2004. Characterization and PCR-based typing of Xanthomonas campestris pv. vesicatoria from peppers and tomatoes in serbia. Eur. J. Plant Pathol. 110:285-292.
  21. Potnis, N., Timilsina, S., Strayer, A., Shantharaj, D., Barak, J. D., Paret, M. L., Vallad, G. E. and Jones, J. B. 2015. Bacterial spot of tomato and pepper: diverse Xanthomonas species with a wide variety of virulence factors posing a worldwide challenge. Mol. Plant Pathol. 16:907-920.
  22. Stall, R. E., Beaulieu, C., Egel, D., Hodge, N. C., Leite, R. P., Minsavage, G. V., Bouzar, H., Jones, J. B., Alvarez, A. M. and Benedict, A. A. 1994. Two genetically diverse groups of strains are included in Xanthomonas campestris pv. vesicatoria. Int. J. Syst. Bacteriol. 44:47-53.
  23. Stoyanova, M., Vancheva, T., Moncheva, P. and Bogatzevska, N. 2014. Differentiation of Xanthomonas spp. causing bacterial spot in Bulgaria based on biolog system. Int. J. Microbiol. 2014:495476.
  24. Sutic, D. 1957. Bakterioze crvenog patlidzana [Tomato bacteriosis]. Posebna Izd. Inst. Zasht. Bilja, Beograd [Spec. Edit. Inst. Plant Prot., Beograd] 6:65.
  25. Timilsina, S., Jibrin, M. O., Potnis, N., Minsavage, G. V., Kebede, M., Schwartz, A., Bart, R., Staskawicz, B., Boyer, C., Vallad, G. E., Pruvost, O., Jones, J. B. and Goss, E. M. 2015. Multilocus sequence analysis of xanthomonads causing bacterial spot of tomato and pepper plants reveals strains generated by recombination among species and recent global spread of Xanthomonas gardneri. Appl. Environ. Microbiol. 81:1520-1529.
  26. Vauterin, L., Hoste, B., Kersters, K. and Swings, J. 1995. Reclassification of Xanthomonas. Int. J. Syst. Evol. Microbiol. 45:472-489.
  27. Yoo, S. H. 2009. List of plant diseases in Korea. 5th ed. The Korean Society of Plant Pathology, Suwon, Korea. p. 76.
  28. Young, J. M., Dye, D. W., Bradbury, J. F., Panagopoulos, C. G. and Robbs, C. F. 1978. A proposed nomenclature and classification for plant pathogenic bacteria. N. Z. J. Agric. Res. 21: 153-177.

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Supported by : Animal and Plant Quarantine Agency, Chungbuk National University