DOI QR코드

DOI QR Code

Determination of Genetic Diversity Using 15 Simple Sequence Repeats Markers in Long Term Selected Japanese Quail Lines

  • Karabag, Kemal (Department of Agricultural Biotechnology, Faculty of Agriculture, Akdeniz University) ;
  • Balcioglu, Murat Soner (Department of Animal Science, Faculty of Agriculture, Akdeniz University) ;
  • Karli, Taki (Department of Animal Science, Faculty of Agriculture, Akdeniz University) ;
  • Alkan, Sezai (Department of Animal Science, Faculty of Agriculture, Ordu University)
  • 투고 : 2015.11.19
  • 심사 : 2016.04.02
  • 발행 : 2016.12.01

초록

Japanese quail is still used as a model for poultry research because of their usefulness as laying, meat, and laboratory animals. Microsatellite markers are the most widely used molecular markers, due to their relative ease of scoring and high levels of polymorphism. The objective of the research was to determine genetic diversity and population genetic structures of selected Japanese quail lines (high body weight 1 [HBW1], HBW2, low body weight [LBW], and layer [L]) throughout 15th generations and an unselected control (C). A total of 69 individuals from five quail lines were genotyped by fifteen microsatellite markers. When analyzed profiles of the markers the observed ($H_o$) and expected ($H_e$) heterozygosity ranged from 0.04 (GUJ0027) to 0.64 (GUJ0087) and 0.21 (GUJ0027) to 0.84 (GUJ0037), respectively. Also, $H_o$ and $H_e$ were separated from 0.30 (L and LBW) to 0.33 (C and HBW2) and from 0.52 (HBW2) to 0.58 (L and LBW), respectively. The mean polymorphic information content (PIC) ranged from 0.46 (HBW2) to 0.52 (L). Approximately half of the markers were informative ($PIC{\geq}0.50$). Genetic distances were calculated from 0.09 (HBW1 and HBW2) to 0.33 (C and L). Phylogenetic dendrogram showed that the quail lines were clearly defined by the microsatellite markers used here. Bayesian model-based clustering supported the results from the phylogenetic tree. These results reflect that the set of studied markers can be used effectively to capture the magnitude of genetic variability in selected Japanese quail lines. Also, to identify markers and alleles which are specific to the divergence lines, further generations of selection are required.

키워드

Simple Sequence Repeats;Selection;Breeding;Genetic Diversity;Quail

과제정보

연구 과제 주관 기관 : Akdeniz University, TUBITAK (The Scientific and Technological Research Council of Turkey)

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