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Association of the Single Nucleotide Polymorphisms in RUNX1, DYRK1A, and KCNJ15 with Blood Related Traits in Pigs

  • Lee, Jae-Bong (Korea Zoonosis Research Institute (KoZRI), Chonbuk National University) ;
  • Yoo, Chae-Kyoung (Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Park, Hee-Bok (National Institute of Animal Science, Rural Development Administration) ;
  • Cho, In-Cheol (National Institute of Animal Science, Rural Development Administration) ;
  • Lim, Hyun-Tae (Institute of Agriculture and Life Science, Gyeongsang National University)
  • Received : 2016.05.03
  • Accepted : 2016.08.03
  • Published : 2016.12.01

Abstract

The aim of this study was to detect positional candidate genes located within the support interval (SI) regions based on the results of red blood cell, mean corpuscular volume (MCV), and mean corpuscular hemoglobin quantitative trait locus (QTL) in Sus scrofa chromosome 13, and to verify the correlation between specific single-nucleotide polymorphisms (SNPs) located in the exonic region of the positional candidate gene and the three genetic traits. The flanking markers of the three QTL SI regions are SW38 and S0215. Within the QTL SI regions, 44 genes were located, and runt-related transcription factor 1, dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 1A (DYRK1A), and potassium inwardly-rectifying channel, subfamily J, member 15 KCNJ15-which are reported to be related to the hematological traits and clinical features of Down syndrome-were selected as positional candidate genes. The ten SNPs located in the exonic region of the three genes were detected by next generation sequencing. A total of 1,232 pigs of an $F_2$ resource population between Landrace and Korean native pigs were genotyped. To investigate the effects of the three genes on each genotype, a mixed-effect model which is the considering family structure model was used to evaluate the associations between the SNPs and three genetic traits in the $F_2$ intercross population. Among them, the MCV level was highly significant (nominal $p=9.8{\times}10^{-9}$) in association with the DYRK1A-SNP1 (c.2989 G$F_2$ intercross, our approach has limited power to distinguish one particular positional candidate gene from a QTL region.

Keywords

Positional Candidate Gene;Blood Related Traits;Association Test;Landrace;Korean Native Pigs

Acknowledgement

Grant : Cooperative Research Program for Agriculture Science and Technology Development

Supported by : Rural Development Administration

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