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Effect of tannin content in sorghum on digestive enzymes

수수의 탄닌 함량이 소화효소에 미치는 영향

  • Bae, Jeong-Suk (Department Crops Breeding, Gyeongsangbuk-do Agricultural Research and Extension Services) ;
  • Ko, Hee-Sun (Department Crops Breeding, Gyeongsangbuk-do Agricultural Research and Extension Services) ;
  • Choi, Hong-Jib (Department Crops Breeding, Gyeongsangbuk-do Agricultural Research and Extension Services) ;
  • Lee, Ji-Yun (Department Crops Breeding, Gyeongsangbuk-do Agricultural Research and Extension Services) ;
  • Kim, Se-Jong (Department Crops Breeding, Gyeongsangbuk-do Agricultural Research and Extension Services)
  • 배정숙 (경상북도농업기술원 작물육종과) ;
  • 고희선 (경상북도농업기술원 작물육종과) ;
  • 최홍집 (경상북도농업기술원 작물육종과) ;
  • 이지윤 (경상북도농업기술원 작물육종과) ;
  • 김세종 (경상북도농업기술원 작물육종과)
  • Received : 2016.08.16
  • Accepted : 2016.10.16
  • Published : 2016.10.30

Abstract

We evaluated the distribution pattern of tannin in 164 sorghum breeding lines and the inhibition rates of amylase, protease, and lipase in sorghum lines with different tannin concentrations. Tannin was existed in the testa of sorghum grain. The tannin content in whole grain of Nampungchal sorghum was 11.54 mg/g, and that in grain (milling rate 73%) and bran fractions was 4.57 mg/g and 28.71 mg/g, respectively. The inhibition rate of ${\alpha}$-amylase, ${\alpha}$-glucosidase, and ${\beta}$-glucosidase in sorghum lines with tannin was higher than that in sorghum lines without tannin. The inhibition rate of ${\alpha}$-glucosidase was greater than 97% in sorghum lines with tannin. The inhibition rate of protease ranged from 20% to 70% in the sorghum lines, showing no discernable trends in tannin content. Lipase inhibition was either very low or not observed and did not seem to correlate with tannin concentration.

본 연구는 수수 계통들의 탄닌 함량별 소화효소 저해율을 조사하여 향후 수수 품종육성과 수수의 이용성 향상을 위하여 실시하였다. 남풍찰수수의 탄닌의 분포를 조사한 결과, 과피와 배유사이의 종피층에 탄닌이 분포함을 알 수 있었다. 도정을 하지 않은 조곡에서 탄닌 함량은 11.54 mg/g이었고, 73% 도정한 종실과 겨에서 각각 4.57 mg/g과 28.71 mg/g으로 가장 높게 나타났다가 65% 도정한 종실과 겨에서 2.06 mg/g과 26.38 mg/g으로 줄었다. 수수 추출물 탄닌 함량별로 15계통을 대상으로 소화효소 반응을 조사한 결과, 탄닌이 함유된 계통들에서 탄닌이 없는 계통들에 비해 당질가수분해 효소인 ${\alpha}$-amylase, ${\alpha}$-glucosidase, ${\beta}$-glucosidase 저해율이 높게 나타났다. 특히 ${\alpha}$-glucosidase의 저해율은 모든 탄닌 계통에서 97% 이상으로 다른 소화효소들에 비해 매우 특이적으로 높은 저해율을 보였다. Protease의 저해율은 탄닌 계통들에서 non-탄닌 계통들에 비해 전반적으로 높게 나타났다. Lipase 저해율은 20% 이하로 저해가 일어나지 않거나 매우 낮아 수수 계통들간의 lipase 저해율의 유의성이 없었다.

Keywords

Acknowledgement

Supported by : 농촌진흥청

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