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Anti-Inflammatory Activity of Pinus koraiensis Cone Bark Extracts Prepared by Micro-Wave Assisted Extraction

  • Kang, Sun-Ae (Food Research Center, Quality Management Team, Hanhwa Hotel & Resort) ;
  • Kim, Dong-Hee (Korea Promotion Institute for Traditional Medicine) ;
  • Hong, Shin-Hyub (School of Food Science and Biotechnology, Kyungpook National University) ;
  • Park, Hye-Jin (School of Food Science and Biotechnology, Kyungpook National University) ;
  • Kim, Na-Hyun (School of Food Science and Biotechnology, Kyungpook National University) ;
  • Ahn, Dong-Hyun (Department of Food Science & Technology, Pukyong National University) ;
  • An, Bong-Jeun (Department of Cosmeceutical Science, Daegu Hanny University) ;
  • Kwon, Joong-Ho (School of Food Science and Biotechnology, Kyungpook National University) ;
  • Cho, Young-Je (School of Food Science and Biotechnology, Kyungpook National University)
  • Received : 2016.04.08
  • Accepted : 2016.06.22
  • Published : 2016.09.30

Abstract

In this study, we compared the anti-inflammatory activity of Pinus koraiensis cone bark extracts prepared by conventional extraction and microwave-assisted extraction (MAE). Water extracts and 50% ethanol extracts prepared using MAE were applied to RAW 264.7 cell at 5, 10, 25, and $50{\mu}g/mL$ of concentrations, and tested for cytoxicity. The group treated with $50{\mu}g/mL$ of 50% ethanol extracts showed toxicity. In order to investigate the inhibition of nitric oxide (NO) production in RAW 264.7 cells, extracts of water and ethanol were treated with 5, 10, and $25{\mu}g/mL$ concentrations. The inhibitory activity of water and 50% ethanol extracts groups were determined as 40% and 60% at $25{\mu}g/mL$ concentration, respectively. We found concentration dependent decreases on inducible NO synthase. The inhibitory effect against forming inflammatory cytokines, prostaglandin $E_2$, tumor necrosis factor-${\alpha}$, interleukin (IL)-6, and IL-$1{\beta}$, was also superior in the $25{\mu}g/mL$ treated group than the control group. According to these results, the water extracts and 50% ethanol extracts both inhibited inflammatory mediators by reducing the inflammatory response. Therefore, The MAE extracts of P. koraiensis cone bark can be developed as a functional ingredient with anti-inflammatory activity.

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