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Trials to Increase the Availability of Ovsynch Program Under Field Conditions in Dairy Cows

  • Jeong, Jae-Kwan (College of Veterinary Medicine, Chungbuk National University) ;
  • Choi, In-Soo (College of Veterinary Medicine, Chungbuk National University) ;
  • Lee, Soo-Chan (College of Veterinary Medicine, Chungbuk National University) ;
  • Kang, Hyun-Gu (College of Veterinary Medicine, Chungbuk National University) ;
  • Hur, Tai-Young (National Institute of Animal Science, RDA) ;
  • Kim, Ill- Hwa (College of Veterinary Medicine, Chungbuk National University)
  • Received : 2016.07.02
  • Accepted : 2016.07.29
  • Published : 2016.08.31

Abstract

This study investigated whether presynchronization with GnRH 6 days before initiation of the Ovsynch program improved reproductive outcomes in dairy cows. Additionally, postponement of initiation of the Ovsynch program for cows during the metestrus phase by 5 days was investigated to determine if it improved reproductive outcomes. To accomplish this, 941 Holstein dairy cows with unknown estrous cycle were randomly allocated into an Ovsynch group (n = 768; $100{\mu}g$ gonadorelin [a GnRH analogue], $500{\mu}g$ of cloprostenol [$PGF_{2{\alpha}}$ analogue] seven days later, $100{\mu}g$ gonadorelin 56 h later and timed artificial insemination [AI] 16 h after) and a G6-Ovsynch (n = 173) that received $100{\mu}g$ GnRH followed by the Ovsynch program 6 days later. Additionally, 272 dairy cows with known estrous cycle (metestrus stage) received the Ovsynch 5 days later (Day 5-Ovsynch group, n = 272). The odds ratio (OR) for pregnancy was analyzed by logistic regression using the LOGISTIC procedure in SAS. The treatment group (p < 0.001) and AI season (p < 0.05) significantly affected the probability of pregnancy, whereas farm, cow parity, calving to AI interval, and body condition score had no affect (p > 0.05). The Day 5-Ovsynch group had a higher probability of pregnancy (OR: 1.71) than the Ovsynch group, while that of the G6-Ovsynch group was intermediate (p > 0.05). Cows inseminated during winter had a higher OR (1.39) than those inseminated during spring. Overall, additional GnRH treatment 6 days before the Ovsynch did not improve reproductive outcomes, whereas postponement of the initiation of Ovsynch by 5 days for cows during metestrus improved reproductive outcomes.

Acknowledgement

Supported by : Rural Development Administration

References

  1. Alkar A, Tibary A, Wenz JR, Nebel RL, Kasimanickam R. Presynchronization with GnRH 7 days prior to resynchronization with CO-Synch did not improve pregnancy rate in lactating dairy cows. Theriogenology 2011; 76: 1036-1041. https://doi.org/10.1016/j.theriogenology.2011.05.006
  2. Al-Katanani YM, Paula-Lopes FF, Hansen PJ. Effect of season and exposure to heat stress on oocyte competence in Holstein cows. J Dairy Sci 2002; 85: 390-396. https://doi.org/10.3168/jds.S0022-0302(02)74086-1
  3. Astiz S, Fargas O. Pregnancy per AI differences between primiparous and multiparous high-yield dairy cows after using double Ovsynch or G6G synchronization protocols. Theriogenology 2013; 79: 1065-1070. https://doi.org/10.1016/j.theriogenology.2013.01.026
  4. Bello NM, Steibel JP, Pursley JR. Optimizing ovulation to first GnRH improved outcomes to each hormonal injection of Ovsynch in lactating dairy cows. J Dairy Sci 2006; 89: 3413-3424. https://doi.org/10.3168/jds.S0022-0302(06)72378-5
  5. Carvalho PD, Fuenzalida MJ, Ricci A, Souza AH, Barletta RV, Wiltbank MC, Fricke PM. Modifications to Ovsynch improve fertility during resynchronization: Evaluation of presynchronization with gonadotropin-releasing hormone 6 d before initiation of Ovsynch and addition of a second prostaglandin $F_{2{\alpha}}$ treatment. J Dairy Sci 2015; 98: 8741-8752. https://doi.org/10.3168/jds.2015-9719
  6. De Rensis F, Scaramuzzi RJ. Heat stress and seasonal effects on reproduction in the dairy cow-a review. Theriogenology 2003; 60: 1139-1151. https://doi.org/10.1016/S0093-691X(03)00126-2
  7. Dewey ST, Mendonca LGD, Lopes G Jr, Rivera FA, Guagnini F, Chebel RC, Bilby TR. Resynchronization strategies to improve fertility in lactating dairy cows utilizing a presynchronization injection of GnRH or supplemental progesterone: I. Pregnancy rates and ovarian responses. J Dairy Sci 2010; 93: 4086-4095. https://doi.org/10.3168/jds.2010-3233
  8. Galvao KN, Sa Filho MF, Santos JEP. Reducing the interval from presynchronization to initiation of timed artificial insemination improves fertility in dairy cows. J Dairy Sci 2007; 90: 4212-4218. https://doi.org/10.3168/jds.2007-0182
  9. Garcia-Ispierto I, Lopez-Gatius F, Bech-Sabat G, Santolaria P, Yaniz JL, Nogareda C, De Rensis F, Lopez-Bejar M. Climate factors affecting conception rate of high producing dairy cows in northeastern Spain. Theriogenology 2007; 67: 1379-1385. https://doi.org/10.1016/j.theriogenology.2007.02.009
  10. Giordano JO, Fricke PM, Guenther JN, Ares MS, Lopes G Jr, Herlihy MM, Fricke PM. Effect of presynchronization with human chorionic gonadotropin or gonadotropin releasing hormone 7 days before resynchronization of ovulation on fertility in lactating dairy cows. J Dairy Sci 2012; 95: 5612-5625. https://doi.org/10.3168/jds.2011-5035
  11. Grimard B, Freret S, Chevallier A, Pinto A, Ponsart C, Humblot P. Genetic and environmental factors influencing first service conception rate and late embryonic/foetal mortality in low fertility dairy herds. Anim Reprod Sci 2006; 91: 31-44. https://doi.org/10.1016/j.anireprosci.2005.03.003
  12. Herlihy MM, Crowe MA, Berry DP, Diskin MG, Butler ST. Factors associated with fertility outcomes in cows treated with protocols to synchronize estrus and ovulation in seasonalcalving, pasture-based dairy production system. J Dairy Sci 2013; 96: 1485-1498. https://doi.org/10.3168/jds.2011-5250
  13. Kim UH, Suh GH, Nam HW, Kang HG, Kim IH. Follicular wave emergence, luteal function and synchrony of ovulation following GnRH or estradiol benzoate in a CIDR-treated, lactating Holstein cows. Theriogenology 2005; 63: 260-268. https://doi.org/10.1016/j.theriogenology.2004.04.005
  14. Lopes G Jr, Giordano JO, Valenza A, Herlihy MM, Guenther JN, Wiltbank MC, Fricke PM. Effect of timing of initiation of resynchronization and presynchronization with gonadotropin-releasing hormone on fertility of resynchronized inseminations in lactating dairy cows. J Dairy Sci 2013; 96: 3788-3798. https://doi.org/10.3168/jds.2012-6429
  15. Lopez-Gatius F, Lopez-Bejar M, Fenech M, Hunter RH. Ovulation failure and double ovulation in dairy cattle: risk factors and effects. Theriogenology 2005; 63: 1298-1307. https://doi.org/10.1016/j.theriogenology.2004.06.010
  16. Lucy MC. Reproductive loss in high-producing dairy cattle: where will it end? J Dairy Sci 2001; 84: 1277-1293. https://doi.org/10.3168/jds.S0022-0302(01)70158-0
  17. Moreira F, Risco C, Pires MFA, Ambrose JD, Drost M, Delorenzo M, Thatcher WW. Effect of body condition on reproductive efficiency of lactating dairy cows receiving a timed insemination. Theriogenology 2000; 53: 1305-1319. https://doi.org/10.1016/S0093-691X(00)00274-0
  18. Moreira F, de la Sota RL, Diaz T, Thatcher WW. Effect of day of the estrous cycle at the initiation of a timed artificial insemination protocol on reproductive responses in dairy heifers. J Anim Sci 2000; 78: 1568-1576. https://doi.org/10.2527/2000.7861568x
  19. Murugavel K, Yaniz JL, Santolaria P, Lopez-Bejar M, Lopez-Gatius F. Luteal activity at the onset of a timed insemination protocol affects reproductive outcome in early postpartum dairy cows. Theriogenology 2003; 60: 583-593. https://doi.org/10.1016/S0093-691X(03)00047-5
  20. Piccardi M, Capitaine Funes A, Balzarini M, Bo GA. Some factors affecting the number of days open in Argentinean dairy herds. Theriogenology 2013; 79: 760-765. https://doi.org/10.1016/j.theriogenology.2012.11.032
  21. Pursley JR, Mee MO, Wiltbank MC. Synchronization of ovulation in dairy cows using $PGF_{2{\alpha}}$ and GnRH. Theriogenology 1995; 44: 915-923. https://doi.org/10.1016/0093-691X(95)00279-H
  22. Pursley JR, Wiltbank MC, Stevenson JS, Ottobre JS, Garverick HA, Anderson LL. Pregnancy rates in cows and heifers inseminated at a synchronized ovulation or synchronized estrus. J Dairy Sci 1997; 80: 295-300. https://doi.org/10.3168/jds.S0022-0302(97)75937-X
  23. Roth Z, Meidan R, Braw-Tal R, Wolfenson D. Immediate and delayed effects of heat stress on follicular development and its association with plasma FSH and inhibin concentration in cows. J Reprod Fertil 2000; 120: 83-90.
  24. Santos JEP, Rutigliano HM, Sa Filho MF. Risk factors for resumption of postpartum estrous cycles and embryonic survival in lactating dairy cows. Anim Reprod Sci 2009; 110: 207-221. https://doi.org/10.1016/j.anireprosci.2008.01.014
  25. Sartori R., Haughian JM, Shaver RD, Rosa GJ, Wiltbank MC. Comparison of ovarian function and circulating steroids in estrous cycles of Holstein heifers and lactating cows. J Dairy Sci 2004; 87: 905-920. https://doi.org/10.3168/jds.S0022-0302(04)73235-X
  26. Vasconcelos JL, Silcox RW, Rosa GJ, Pursley JR, Wiltbank MC. Synchronization rate, size of the ovulatory follicle, and pregnancy rate after synchronization of ovulation beginning on different days of the estrous cycle in lactating dairy cows. Theriogenology 1999; 52: 1067-1078. https://doi.org/10.1016/S0093-691X(99)00195-8
  27. Whittier WD, Gwazdauskas FC, McGilliard ML. Prostaglandin $F_{2{\alpha}}$ usage in a dairy reproduction program for treatment of unobserved estrus, pyometra and ovarian luteal cysts. Theriogenology 1989; 32: 693-704. https://doi.org/10.1016/0093-691X(89)90290-2