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Analysis of Bacterial Diversity in Water from the Han River Water Source Protection Area via a Pyrosequencing Assay

파이로시퀀싱을 이용한 한강상수원보호구역 수계 중의 세균 다양성

  • Kim, Heejung (School of Earth and Environmental Sciences, Seoul National University) ;
  • Kaown, Dugin (School of Earth and Environmental Sciences, Seoul National University) ;
  • Kim, Changsoo (Water Supply & Sewerage Research Division, National Institute of Environmental Research) ;
  • Lee, Siwon (Water Supply & Sewerage Research Division, National Institute of Environmental Research)
  • 김희정 (서울대학교 지구환경과학부) ;
  • 권덕인 (서울대학교 지구환경과학부) ;
  • 김창수 (국립환경과학원 상하수도연구과) ;
  • 이시원 (국립환경과학원 상하수도연구과)
  • Received : 2016.07.22
  • Accepted : 2016.08.16
  • Published : 2016.08.31

Abstract

Objectives: We investigated bacterial diversity in the Han River water resource protection area in order to provide basic microbiological information on the drinking water safety of the Seoul metropolitan region. Methods: Samples were collected in the spring and winter, but not during the rainy season. Pyrosequencing, gene amplification, and extraction of nucleic acids were employed in this study. Results: In total, 57 and 48 operational taxonomic units were respectively analyzed in samples collected during spring and winter. Proteobacteria were predominant in all samples. The samples contained phylogenetically diverse bacterial communities, with eleven major phyla and 36 genera. Cyanobacteria were predominant in the spring samples, but not in the winter samples. The predominant species in the samples collected during both seasons belonged to the genus Aquamicrobium and Bradyrhizobium. Moreover, no pathogenic bacteria were detected in the samples. Conclusion: Proteobacteria were predominant in the samples from the Han River water source protection area. Cyanobacteria were more predominant in the spring samples than in the winter samples, but Aquamicrobium and Bradyrhizobium were predominant in both sampling seasons.

Acknowledgement

Supported by : 한국연구재단

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