Preparation and Characterization of an Antibody Antagonist That Targets the Porcine Growth Hormone Receptor

  • Cui, Huanzhong (College of Animal Science and Technology Jilin Agricultural University) ;
  • Wang, Yanrong (Henan University of Science and Technology) ;
  • Song, Meng (College of Animal Science and Technology Jilin Agricultural University) ;
  • Zhang, Hui (College of Animal Science and Technology Jilin Agricultural University)
  • Received : 2015.10.28
  • Accepted : 2015.11.30
  • Published : 2016.10.01


A series of antagonists specifically targeting growth hormone receptors (GHR) in different species, such as humans, rats, bovines, and mice, have been designed; however, there are currently no antagonists that target the porcine growth hormone (GH). Therefore, in this study, we developed and characterized a porcine GHR (pGHR) antibody antagonist (denoted by AN98) via the hybridoma technique. The results from enzyme-linked immunosorbent assay, fluorescence activated cell sorter, indirect immunoinfluscent assay, and competitive receptor binding analysis showed that AN98 could specifically recognize pGHR, and further experiments indicated that AN98 could effectively inhibit pGH-induced signalling in CHO-pGHR cells and porcine hepatocytes. In addition, AN98 also inhibited GH-induced insulin-like growth factor-1 (IGF-1) secretion in porcine hepatocytes. In summary, these findings indicated that AN98, as a pGHR-specific antagonist, has potential applications in pGH-pGHR-related research on domestic pigs.


Porcine Growth Hormone;Porcine Growth Hormone Receptors;Signalling Pathway;Antagonist


Supported by : Natural Science Foundation


  1. Sun, F., Y. Liu, H. Sun, and B. Tian. 2015. Development and characterization of a novel GHR antibody antagonist, GF185. Int. J. Biol. Macromol. 79:864-870.
  2. Van der Lely, A. J., A. F. Muller, J. A. Janssen, R. J. Davis, K. A. Zib, J. A. Scarlett, and S. W. Lamberts. 2001. Control of tumor size and disease activity during cotreatment with octreotide and the growth hormone receptor antagonist pegvisomant in an acromegalic patient. J. Clin. Endocrinol. Metab. 86:478-481.
  3. Abdel-Meguid, S. S., H. S. Shieh, W. W. Smith, H. E. Dayriner, B. N. Violand, and L. A. Bentle. 1987. Three dimensional structure of a genetically engineered variant of porcine growth hormone. Proc. Natl. Acad. Sci. USA. 84:6434-6437.
  4. Barnard, R., P. G. Bundesen, D. B. Rylatt, and M. J. Waters. 1984. Monoclonal antibodies to the rabbit liver growth hormone receptor: Production and characterization. Endocrinology 115:1805-1813.
  5. Brooks, A. J. and M. J. Waters. 2010. The growth hormone receptor: mechanism of activation and clinical implications. Nat. Rev. Endocrinol. 6:515-525.
  6. Brooks, A. J., W. Dai, M. L. O'Mara, D. Abankwa, Y. Chhabra, R. A. Pelekanos, O. Gardon, K. A. Tunny, K. M. Blucher, and C. J. Morton et al. 2014. Mechanism of activation of protein kinase JAK2 by the growth hormone receptor. Science 344:1249783.
  7. Chen, W. Y., D. C. Wight, B. V. Mehta, T. E. Wagner, and J. J. Kopchick. 1991. Glycine 119 of bovine growth hormone is critical for growth-promoting activity. Mol. Endocrinol. 5:1845-1852.
  8. Chen, W. Y., N. Y. Chen, J. Yun, T. E. Wagner, and J. J. Kopchick. 1994. In vitro and in vivo studies of antagonistic effects of human growth hormone analogs. J. Biol. Chem. 269:15892-15897.
  9. Chung, C. S., T. D. Etherton, and J. P. Wiggins. 1985. Stimulation of swine growth by porcine growth hormone. J. Anim. Sci. 60:118-130.
  10. de Vos, A. M., M. Ultsch, and A. A. Kossiakoff. 1992. Human growth hormone and extracellular domain of its receptor:crystal structure of the complex. Science 255:306-312.
  11. Gatford, K. L., J. M. Boyce, K. Blackmore, R. J. Smits, R. G. Campbell, and P. C. Owens. 2004. Long-term, but not shortterm, treatment with somatotropin during pregnancy in underfed pigs increases the body size of progeny at birth. J. Anim. Sci. 82:93-101.
  12. Lan, H. N., H. L. Jiang, W. Li, T. C. Wu, P. Hong, Y. M. Li, H. Zhang, H. Z. Cui, and X. Zheng. 2015a. Development and characterization of a novel anti-idiotypic monoclonal antibody to growth hormone, which can mimic physiological functions of growth hormone in primary porcine hepatocytes. Asian Australas. J. Anim. Sci. 28:573-583.
  13. Lan, H. N., W. Li, Z. Fu, Y. Yang, T. Wu, Y. Liu, H. Zhang, H. Cui, Y. Li, P. Hong, J. Liu, and X. Zheng. 2014. Differential intracellular signalling properties of the growth hormone receptor induced by the activation of an anti-GHR antibody. Mol. Cell. Endocrinol. 390:54-64.
  14. Lan, H. N., X. Zheng, M. A. Khan, and S. Li. 2015b. Antiidiotypic antibody: A new strategy for the development of a growth hormone receptor antagonist. Int. J. Biochem. Cell. Biol. 68:101-108.
  15. Ramsay, T. G. and M. P. Richards. 2005. Leptin and leptin receptor expression in skeletal muscle and adipose tissue in response to in vivo porcine somatotropin treatment. J. Anim. Sci. 83:2501-2508.