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Preparation and Characterization of an Antibody Antagonist That Targets the Porcine Growth Hormone Receptor

  • Cui, Huanzhong (College of Animal Science and Technology Jilin Agricultural University) ;
  • Wang, Yanrong (Henan University of Science and Technology) ;
  • Song, Meng (College of Animal Science and Technology Jilin Agricultural University) ;
  • Zhang, Hui (College of Animal Science and Technology Jilin Agricultural University)
  • Received : 2015.10.28
  • Accepted : 2015.11.30
  • Published : 2016.10.01

Abstract

A series of antagonists specifically targeting growth hormone receptors (GHR) in different species, such as humans, rats, bovines, and mice, have been designed; however, there are currently no antagonists that target the porcine growth hormone (GH). Therefore, in this study, we developed and characterized a porcine GHR (pGHR) antibody antagonist (denoted by AN98) via the hybridoma technique. The results from enzyme-linked immunosorbent assay, fluorescence activated cell sorter, indirect immunoinfluscent assay, and competitive receptor binding analysis showed that AN98 could specifically recognize pGHR, and further experiments indicated that AN98 could effectively inhibit pGH-induced signalling in CHO-pGHR cells and porcine hepatocytes. In addition, AN98 also inhibited GH-induced insulin-like growth factor-1 (IGF-1) secretion in porcine hepatocytes. In summary, these findings indicated that AN98, as a pGHR-specific antagonist, has potential applications in pGH-pGHR-related research on domestic pigs.

Keywords

Porcine Growth Hormone;Porcine Growth Hormone Receptors;Signalling Pathway;Antagonist

Acknowledgement

Supported by : Natural Science Foundation

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