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Reversal of Multidrug Resistance and Computational Studies of Pistagremic Acid Isolated from Pistacia integerrima

  • Rauf, Abdur (Department of Geology, University of Swabi) ;
  • Uddin, Ghias (Institute of Chemical Sciences, University of Peshawar) ;
  • Raza, Muslim (State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology) ;
  • Ahmad, Aftab (State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology) ;
  • Jehan, Noor (Department of Geology, University of Swabi) ;
  • Ahmad, Bashir (Centre of Biotechnology & Microbiology, University of Peshawar) ;
  • Nisar, Muhammad (Institute of Chemical Sciences, University of Peshawar) ;
  • Molnar, Joseph (Department of Medical Microbiology and Immunobiology, Faculty of Medicine, University of Szeged) ;
  • Csonka, Akos (Department of Medical Microbiology and Immunobiology, Faculty of Medicine, University of Szeged) ;
  • Szabo, Diana (Department of Medical Microbiology and Immunobiology, Faculty of Medicine, University of Szeged) ;
  • Khan, Ajmal (Department of Chemistry, COMSAT, Institute of Information Technology) ;
  • Farooq, Umar (Department of Chemistry, COMSAT, Institute of Information Technology) ;
  • Noor, Mah (Centre of Biotechnology & Microbiology, University of Peshawar)
  • Published : 2016.06.01

Abstract

Pistagremic acid (PA) is a bioactive triterpenoid isolated from various parts of Pistacia integerrima plants. The aim of this research was to investigate PA for reversion of multidrug resistant (MDR) mediated by P-glycoprotein using rhodamine-123 exclusion study on a multidrug resistant human ABCB1 (ATP-binding cassette, sub-family B, member 1) gene-transfected mouse T-lymphoma cell line in vitro. Results were similar to those with verapamil as a positive control. Docking studies of PA and standard Rhodamine123 were carried out against a P-gp crystal structure which showed satisfactory results. Actually, PA cannot bind exactly where co-crystallized ligand of P-gp is already present. However, the docking study predicted that if a compound gives a lesser score then it may have some potency. The docking scores of PA and Rhodamine were similar. Therefore, we can conclude that there are certain important chemical features of PA which are responsible for the inhibiting potency of P-gp.

Keywords

Pistacia integerrima;pistagremic acid;reversal of MDR in mouse lymphoma cells activities;molecular

Acknowledgement

Supported by : Higher Education Commission of Pakistan, Szeged Foundation for Cancer Research, European Social Fund

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