- Volume 17 Issue 4
DOI QR Code
Distribution of Glutathione S-Transferase Omega Gene Polymorphism with Different Stages of HBV Infection Including Hepatocellular Carcinoma in the Egyptian Population
- Shaban, Nadia Z (Biochemistry Department, Faculty of Science, Alexandria University) ;
- Salem, Halima H (Nucleic Acid Research Department, Genetic Engineering and Biotechnology Research Institute, City of Scientific Research and Technological Applications) ;
- Elsadany, Mohamed A (Biochemistry Department, Faculty of Science, Alexandria University) ;
- Ali, Bahy A (Nucleic Acid Research Department, Genetic Engineering and Biotechnology Research Institute, City of Scientific Research and Technological Applications) ;
- Hassona, Ehab M (Internal Medicine Department, Faculty of Medicine, Alexandria University) ;
- Mogahed, Fayed AK (Nucleic Acid Research Department, Genetic Engineering and Biotechnology Research Institute, City of Scientific Research and Technological Applications)
- Published : 2016.06.01
Background: Infection with hepatitis B virus (HBV) is a major global public health problem, with a wide spectrum of clinical manifestations. Human cytosolic glutathione-S-transferases (GSTs) include several classes such as alpha (A), mu (M), pi (P), sigma (S), zeta (Z), omega (O) and theta (T). The present study aimed to investigate the role of GST omega genes (GSTO1 and GSTO2) in different groups of patients infected with HBV. Materials and Methods: HBV groups were classified according to clinical history, serological tests and histological analysis into normal carriers (N), acute (A), chronic (CH), cirrhosis (CI) and hepatocellular carcinoma (HCC) cases. The study focused on determination of the genotypes of GST omega genes (GSTO1 and GSTO2) and GST activity and liver function tests. Results: The results showed that GSTO1 (A/A) was decreased in N, A, CH, CI and HCC groups compared to the C-group, while, GSTO1 (C/A) and GSTO1(C/C) genotypes were increased significantly in N, A, CH, CI and HCC groups. GSTO2 (A/A) was decreased in all studied groups as compared to the C-group but GSTO2(A/G) and GSTO2(G/G) genotypes were increased significantly. In addition, GST activities, albumin and TP levels were decreased in all studied groups compared to the C-group, while the activities of transaminases were increased to differing degrees. Conclusions: The results indicate that GSTO genetic polymorphisms may be considered as biomarkers for determining and predicting the progression of HBV infection.
HBV;infection;cirrhosis;HCC;GST;omega genes;genetic polymorphisms
- Ada TG, Ada AO, Kunak SC, et al (2013). Association between glutathione S-transferase omega 1 A140D polymorphism in the Turkish population and susceptibility to non-small cell lung cancer. Arh Hig Rada Toksikol, 64, 247-53. https://doi.org/10.2478/10004-1254-64-2013-2302
- Azarpira N, Nikeghbalian S, Geramizadeh B (2010). Influence of glutathione S-transferase M1 and T1 polymorphisms with acute rejection in Iranian liver transplant recipients. Mol Biol Rep, 37, 21-25. https://doi.org/10.1007/s11033-009-9487-5
- Becket GJ, Dyson EH, Chapman BJ, et al (1997). Plasma glutathione S-transferase measurements and liver disease in man. J Clin Biochem Nutr, 2, 1-24.
- Board PG, Coggan M, Chelvanayagam G, et al (2000). Identification, characterization and crystal structure of the omega class glutathione transferases. J Biol Chem, 275, 24798-806. https://doi.org/10.1074/jbc.M001706200
- Chisari FV, Ferrari C (1995). Hepatitis B virus immunopathogenesis. Ann Rev of Immunol, 13, 29-60.
- Chu CJ, Lok ASF (2002). Clinical utility in quantifying serum HBV DNA levels using PCR assay. J hepatol, 36, 549-51. https://doi.org/10.1016/S0168-8278(02)00039-9
- Danil S, Pratt MD, Marshal M (1999). Evaluation of the liver: laboratory tests. in Schiff's Diseases of the Liver, 8th edn. USA; JB Lippincott Publications, 205, 39.
- Dulhunty A, Gage P, Curtis S, et al (2001). Glutathione transferase structural family includes a nuclear chloride channel and a ryanodine receptor calcium release channel modulator. J Biol chem, 276, 3319-23. https://doi.org/10.1074/jbc.M007874200
- Habig WH, Pabst J, Jakoby B (1974). Glutathione S-transferases. The first enzymatic step in mercapturic acid formation. J Biol Chem, 249, 7130-39.
- Hassan MM, Li D, El-Deeb AS (2008). Association between hepatitis B virus and pancreatic cancer. J Clin Oncol, 26, 4557-62. https://doi.org/10.1200/JCO.2008.17.3526
- Heo J, Baik TH, Kim HH, et al (2003). Serum hepatitis B virus (HBV) DNA levels at different stages of clinical course in patients with chronic HBV infection in an endemic area. J Korean Med Sci, 18, 686-90. https://doi.org/10.3346/jkms.2003.18.5.686
- Li YJ, Sofia AO, Puting X, et al (2003). Glutathione S-transferase omega-1 modifies age-at-onset of Alzheimer disease and Parkinson disease. Human Mol Gen, 12, 3259-67. https://doi.org/10.1093/hmg/ddg357
- Lindh M, Horal P, Dhillon AP, et al (2000). Hepatitis B virus DNA levels, precore mutations, genotypes and histological activity in chronic hepatitis B. J Vir Hepat,7, 258-67. https://doi.org/10.1046/j.1365-2893.2000.00236.x
- Locarnini SA, Shaw T (2004). Antiviral drug resistance in hepatitis B and C. J Gastroenterol and Hepatol, 9, 322-28.
- Marahatta SB, Punyarit P, Bhudisawasdi V, et al (2006). Polymorphism of glutathione S-transferase omega gene and risk of cancer. Canc Lett, 236, 276-81. https://doi.org/10.1016/j.canlet.2005.05.020
- Masoudi M, Saadat I, Omidvari SH(2009). Genetic polymorphisms of GSTO2, GSTM1, and GSTT1 and risk of gastric cancer. Mol Biol Rep, 36, 781-84. https://doi.org/10.1007/s11033-008-9245-0
- Masoudi M, Saadat I, Omidvari S, et al (2011). Association between N142D genetic polymorphism of GSTO2 and susceptibility to colorectal cancer. Mol Biol Rep, 38, 4309-13. https://doi.org/10.1007/s11033-010-0555-7
- Miller SA, Dykes DD, Polesky HF (1998) . A simple salting out procedure for extracting DNA from human nucleated cells. Nucl Acid Res, 16, 3.
- Mukherjee B, Oreste ES, Linda LP, et al (2006). Glutathione S-transferase omega 1 and omega 2 pharmacogenomics. Am Soc Pharmacol Expert Therap, 34, 1237-46.
- Oslen A, Autrup H, Sorensen M , et al (2008). Polymorphisms of glutathione S-transferase A1 and O1 and breast cancer among postmenopausal Danish women. Europ J Canc Prev, 17, 225 -29. https://doi.org/10.1097/CEJ.0b013e3282b6fe1e
- Polimantia R, Piacentinia S, De Angelisa F, et al (2011). Human GST loci as markers of evolution nary forces: GSTO1*E155del and GSTO1*E208K polymorphisms may be under natural selection induced by environmental arsenic. Dis Mark, 31, 231-39. https://doi.org/10.1155/2011/124169
- Pongstaporn W, Pakakasama S (2009). Polymorphism of glutathione S-transferase Omega gene: association with risk of childhood acute lymphoblastic leukemia. J. Canc Res and Clin Oncol, 135, 673-78. https://doi.org/10.1007/s00432-008-0501-4
- Pongstaporn W, Pakakasama S, Sanguansin S, et al (2008). Polymorphism of glutathione S-transferase omega gene: association with risk of childhood acute lymphoblastic leukemia. J Cancer Res Clin Oncol, 432, 501-04.
- Pongstaporn W, Rochanawutanon M, Wilailak S, et al (2006). Genetic alterations in chromosome 10q24.3 and glutathione S-transferase omega 2 gene polymorphism in ovarian cancer. J Exp Clin Cancer Res, 25, 107-14.
- Schmuck EM, Board PG, Whitbread AK, et al(2005). Characterization of the monomethylarsonate reductase and dehydroascorbate reductase activities of omega class glutathione transferase variants: implications for arsenic metabolism and the age-at-onset of Alzheimer’s and Parkinson’s diseases. Pharmacogen Genom, 15, 493-01. https://doi.org/10.1097/01.fpc.0000165725.81559.e3
- Shaban NZ, Abdul-Aziz AA (2014a). Effect of Meloxicam on Lipoxygenase Activity in Trachea, Skin, Stomach and Serum of Rabbits (in Vivo and in Vitro). Life Sci J, 11, 48-55.
- Shaban NZ, El-Kersh MA, Bader-Eldin MM, et al (2014b). Effect of punica granatum (pomegranate) juice extract on healthy liver and hepatotoxicity induced by diethylnitrosamine and phenobarbital in male Rats. J Med Food, 17, 339-49. https://doi.org/10.1089/jmf.2012.0306
- Shaban NZ, El-Kersh MA, El-Rashidy FH, et al (2013). Protective role of Punica granatum (pomegranate) peel and seed oil extracts on diethylnitrosamine and phenobarbitalinduced hepatic injury in male rats. Food Chem, 141, 1587-96. https://doi.org/10.1016/j.foodchem.2013.04.134
- Shaban NZ, Helmy MH, El-Kersh MA, et al (2003). Effects of Bacillus thuringiensis toxin on hepatic lipid peroxidation and freeradical scavengers in rats given alpha-tocopherol or acetylsalicylate. comparative biochemistry and physiology part C. Toxicol Pharmacol, 135, 405-14.
- Shaban NZ, Salem HM, Elsaadany MA, et al (2014c). Alteration in lipid peroxidation and antioxidant in patients with different stages of hepatitis B virus infection in Egypt. Life Sci J, 11, 960-67.
- Shaban NZ, Salem HM, Elsaadany MA, et al (2014d). Association between the genetic polymorphism of glutathione S -transferase genes and the different stages of hepatitis B virus infection in Egypt. Life Sci J, 11, 723-30.
- Strange RC, Strange MA, Spiteri S, et al (2001). Glutathione-Stransferase family of enzymes. Mutat Res, 482, 21-6. https://doi.org/10.1016/S0027-5107(01)00206-8
- Wang FS (2003). Current status and prospects of studies on human genetic alleles associated with hepatitis B virus infection. W J Gastroenterol, 9, 641-44. https://doi.org/10.3748/wjg.v9.i4.641
- Whitbread AK, Masoumi A, Tetlow N, et al (2005). Characterization of the omega class of glutathione transferases. Method Enzymol, 401, 78-99. https://doi.org/10.1016/S0076-6879(05)01005-0
- Whitbread AK, Tetlow N, Eyre HJ, et al (2003). Characterization of the human omega class glutathione transferase genes and associated polymorphisms. Pharmacogen, 13, 131-44. https://doi.org/10.1097/00008571-200303000-00003
- William W (1997). Effect of pH and ionic strength on the spectrophotometric assessment of nucleic acid purity. Biotech, 22, 474-81.
- Yuen MF, Oi-Lin NI, Fan ST (2004). Significance of HBV DNA levels in liver histology of HBeAg and anti-HBe positive patients with chronic hepatitis B. Amer J Gastroenterol, 99, 203-27. https://doi.org/10.1111/j.1572-0241.2004.04036.x