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Immuno-Modulatory Activities of Polysaccharides separated from Chrysanthemum zawadskii var. latilobum in Macrophage Cells

구절초(Chrysanthemum zawadskii var. latilobum)에서 분리된 다당류의 대식세포 면역조절 활성

  • Sung, Nak-Yun (Dept. of Food Science and Technology, Kongju National University) ;
  • Park, Yoo-Young (Dept. of Food Science and Technology, Kongju National University) ;
  • Kim, Yi-Eun (Dept. of Food Science and Technology, Kongju National University) ;
  • Cho, Eun-Ji (Dept. of Food Science and Technology, Kongju National University) ;
  • Kim, Mi-Hwan (Dept. of Food Science and Technology, Kongju National University) ;
  • Ryu, Gi-Hyung (Dept. of Food Science and Technology, Kongju National University) ;
  • Byun, Eui-Hong (Dept. of Food Science and Technology, Kongju National University) ;
  • Park, Youn-Je (Dept. of Food Science and Technology, Kongju National University)
  • Received : 2016.05.02
  • Accepted : 2016.06.22
  • Published : 2016.06.30

Abstract

Macrophages play a pivotal role in the innate and adaptive immune systems. This study investigated the immuno-modulatory activities of polysaccharides separated from Chrysanthemum zawadskii var. latilobum (CZPS) in macrophages. Polysaccharides from Chrysanthemum zawadskii var. latilobum were extracted by the ethanol precipitation method. RAW 264.7 mouse macrophage cell line was treated with CZPS (4 to $128{\mu}g/mL$), and there was no cytotoxicity at a dose below $32{\mu}g/mL$. The levels of nitric oxide (NO) and pro-inflammatory cytokines (tumor necrosis factor (TNF)-${\alpha}$ and interleukin (IL)-6, IL-$1{\beta}$) production in the CZPS treated group ($32{\mu}g/mL$) were $6.5{\pm}0.12{\mu}m$ (NO), $1252.8{\pm}79.85$ (TNF-${\alpha}$), $305.4{\pm}29.41$ (IL-6), and $683.3{\pm}59.71$ (IL-$1{\beta}$), respectively, and they were significantly increased when compared to the control group; $2.2{\pm}0.03{\mu}m$ (NO), $452.3{\pm}38.34$ (TNF-${\alpha}$), $31.7{\pm}5.75$ (IL-6), and $184.1{\pm}11.52$ (IL-$1{\beta}$). Additionally, protein expression of inducible nitric oxide synthase (iNOS) and phosphorylation of MAPKs and NF-${\kappa}B$ expression were significantly increased upon CZPS treatment. Therefore, these results indicated that polysaccharides separated from Chrysanthemum zawadskii var. latilobum (CZPS) may have a potential immunomodulatory activity in macrophages through MAPKs and NF-${\kappa}B$ signaling, and this information is useful for the development of immune enhancing adjuvant materials using a natural ingredient.

Acknowledgement

Supported by : 한국산업기술진흥원

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