Evaluation of Relative Bioavailability of 25-Hydroxycholecalciferol to Cholecalciferol for Broiler Chickens

  • Han, J.C. (Department of Animal Science, College of Life Science, Shangqiu Normal University) ;
  • Chen, G.H. (Department of Animal Science, College of Life Science, Shangqiu Normal University) ;
  • Wang, J.G. (Department of Animal Science, College of Life Science, Shangqiu Normal University) ;
  • Zhang, J.L. (Department of Animal Science, College of Life Science, Shangqiu Normal University) ;
  • Qu, H.X. (Department of Animal Science, College of Life Science, Shangqiu Normal University) ;
  • Zhang, C.M. (Department of Animal Science, College of Life Science, Shangqiu Normal University) ;
  • Yan, Y.F. (Department of Animal Science, College of Life Science, Shangqiu Normal University) ;
  • Cheng, Y.H. (Department of Biotechnology and Animal Science, National Ilan University)
  • Received : 2015.07.02
  • Accepted : 2015.10.14
  • Published : 2016.08.01


This study was conducted to evaluate the relative bioavailability (RBV) of 25-hydroxycholecalciferol (25-OH-$D_3$) to cholecalciferol (vitamin $D_3$) in 1- to 21-d-old broiler chickens fed with calcium (Ca)- and phosphorus (P)-deficient diets. On the day of hatch, 450 female Ross 308 broiler chickens were assigned to nine treatments, with five replicates of ten birds each. The basal diet contained 0.50% Ca and 0.25% non-phytate phosphorus (NPP) and was not supplemented with vitamin D. Vitamin $D_3$ was fed at 0, 2.5, 5.0, 10.0, and $20.0{\mu}g/kg$, and 25-OH-$D_3$ was fed at 1.25, 2.5, 5.0, and $10.0{\mu}g/kg$. The RBV of 25-OH-$D_3$ was determined using vitamin $D_3$ as the standard source by the slope ratio method. Vitamin $D_3$ and 25-OH-$D_3$ intake was used as the independent variable for regression analysis. The linear relationships between the level of vitamin $D_3$ or 25-OH-$D_3$ and body weight gain (BWG) and the weight, length, ash weight, and the percentage of ash, Ca, and P in femur, tibia, and metatarsus of broiler chickens were observed. Using BWG as the criterion, the RBV value of 25-OH-$D_3$ to vitamin $D_3$ was 1.85. Using the mineralization of the femur, tibia, and metatarsus as criteria, the RBV of 25-OH-$D_3$ to vitamin $D_3$ ranged from 1.82 to 2.45, 1.86 to 2.52, and 1.65 to 2.05, respectively. These data indicate that 25-OH-$D_3$ is approximately 2.03 times as active as vitamin $D_3$ in promoting growth performance and bone mineralization in broiler chicken diets.


25-Hydroxycholecalciferol;Cholecalciferol;Relative Bioavailability;Growth;Bone;Broiler Chicken


Supported by : National Natural Science Foundation of China, Education Department of Henan Province, Shangqiu Normal University Foundation


  1. Aburto, A., H. M. Edwards Jr., and W. M. Britton. 1998. The influence of vitamin A on the utilization and amelioration of toxicity of cholecalciferol, 25-hydroxycholecalciferol, and 1,25 dihydroxycholecalciferol in young broiler chickens. Poult. Sci. 77:585-593.
  2. Applegate, T. J. and M. S. Lilburn. 2002. Growth of the femur and tibia of a commercial broiler line. Poult. Sci. 81:1289-1294.
  3. Atencio, A., G. M. Pesti, and H. M. Edwards. Jr. 2005. Twenty-five hydroxycholecalciferol as a cholecalciferol substitute in broiler breeder hen diets and its effect on the performance and general health of the progeny. Poult. Sci. 84:1277-1285.
  4. Baker, D. H., R. R. Biehl, and J. L. Emmert. 1998. Vitamin $D_3$ requirement of young chicks receiving diets varying in calcium and available phosphorus. Br. Poult. Sci. 39:413-417.
  5. Bar, A., D. Shinder, S. Yosefi, E. Vax, and I. Plavnik. 2003. Metabolism and requirements for calcium and phosphorus in the fast-growing chicken as affected by age. Br. J. Nutr. 89:51-60.
  6. Biehl, R. R. and D. H. Baker. 1997. Utilization of phytate and nonphytate phosphorus in chicks as affected by source and amount of vitamin $D_3$. J. Anim. Sci. 75:2986-2993.
  7. Edwards Jr., H. M. 2002. Studies on the efficacy of cholecalciferol and derivatives for stimulating phytate utilization in broilers. Poult. Sci. 81:1026-1031.
  8. Fritts, C. A. and P. W. Waldroup. 2003. Effect of source and level of vitamin D on live performance and bone development in growing broilers. J. Appl. Poult. Res. 12:45-52.
  9. Goetting-Fuchs, C., R. Gunther, V. G. Liesner, B. G. Liesner, M. Beyerbach, and J. Kamphues. 2012. Investigations on skeletal development, bone mineralisation as well as calcium and phosphorus levels in blood of male fattening turkeys. Europ. Poult. Sci. 76:121-130.
  10. Goodgame, S. D., F. J. Mussini, C. Lu, C. D. Bradley, S. E. Watkins, and P. W. Waldroup. 2011. Evaluation of a fermentation source of 25-hydroxycholecalciferol in broiler diets. Int. J. Poult. Sci. 10:295-299.
  11. Hall, L. E., R. B. Shirley, R. I. Bakalli, S. E. Aggrey, G. M. Pesti, and H. M. Edwards. Jr. 2003. Power of two methods for the estimation of bone ash of broilers. Poult. Sci. 82:414-418.
  12. Han, J. C., H. X. Qu, J. G. Wang, G. H. Chen, Y. F. Yan, J. L. Zhang, F. M. Hu, L. Y. You, and Y. H. Cheng. 2015. Comparison of the growth and mineralization of the femur, tibia, and metatarsus of broiler chicks. Braz. J. Poult. Sci. 17:333-339.
  13. Han, J. C., H. X. Qu, J. Q. Wang, J. H. Yao, C. M. Zhang, G. L. Yang, Y. H. Cheng, and X. S. Dong. 2013. The effects of dietary cholecalciferol and $1{\alpha}$-hydroxycholecalciferol levels in a calcium- and phosphorus-deficient diet on growth performance and tibia quality of growing broilers. J. Anim. Feed Sci. 22:158-164.
  14. Han, J. C., Y. Liu, J. H. Yao, J. Q. Wang, H. X. Qu, Y. F. Yan, J. Yue, J. L. Ding, Z. T. Shi, and X. S. Dong. 2012. Dietary calcium levels reduce the efficacy of one alpha-hydroxycholecalciferol in phosphorus-deficient diets of broilers. J. Poult. Sci. 49:34-38.
  15. Jendral, M. J., D. R. Korver, J. S. Church, and J. J. R. Feddes. 2008. Bone mineral density and breaking strength of white leghorns housed in conventional, modified, and commercially available colony battery cages. Poult. Sci. 87:828-837.
  16. Koreleski, J. and S. Swiatkiewicz. 2005. Efficacy of different limestone particle size and 25-hydroxycholecalciferol in broiler diets. J. Anim. Feed Sci. 14:705-714.
  17. Ledwaba, M. F. and K. D. Roberson. 2003. Effectiveness of twenty five hydroxycholecalciferol in the prevention of tibial dyschondroplasia in Ross cockerels depends on dietary calcium level. Poult. Sci. 82:1769-1777.
  18. Littell, R. C., P. R. Henry, A. J. Lewis, and C. B. Ammerman. 1997. Estimation of relative bioavailability of nutrients using SAS procedures. J. Anim. Sci. 75:2672-2683.
  19. Qian, H., E. T. Kornegay, and D. M. Denbow. 1997. Utilization of phytate phosphorus and calcium as influenced by microbial phytase, cholecalciferol, and the calcium: Total phosphorus ratio in broiler diets. Poult. Sci. 76:37-46.
  20. Rao, S. V. R., M. V. L. N. Raju, A. K. Panda, G. S. Sunder, and R. P. Sharma. 2006. Effect of high concentrations of cholecalciferol on growth, bone mineralization, and mineral retention in broiler chicks fed suboptimal concentrations of calcium and nonphytate phosphorus. J. Appl. Poult. Res. 15:493-501.
  21. Rao, S. V. R., M. V. L. N. Raju, A. K. Panda, G. S. Sunder, and R. P. Sharma. 2009. Performance and bone mineralisation in broiler chicks fed on diets with different concentrations of cholecalciferol at a constant ratio of calcium to non-phytate phosphorus. Br. Poult. Sci. 50:528-535.
  22. Rao, S. V. R., M. V. L. N. Raju, and M. R. Reddy. 2007. Performance of broiler chicks fed high levels of cholecalciferol in diets containing sub-optimal levels of calcium and non-phytate phosphorus. Anim. Feed Sci. Tech. 134:77-88.
  23. SAS Institute. 2002. SAS User's Guide. 9th edn. SAS Inst. Inc., Cary, NC, USA.
  24. Shirley, R. B. 2003. Evaluation of Phytase, Vitamin D3 Derivatives, and Broiler Breed Differences on Nutrient Utilization, Broiler Performance, Leg Disorders, and the Expression of Intestinal Calbindin-28 kd mRNA and Protein. Ph.D. Dissertation. University of Georgia, Athens, GA, USA.
  25. Soares Jr., J. H., J. M. Kerr, and R. W. Gray. 1995. 25-hydroxycholecalciferol in poultry nutrition. Poult. Sci. 74:1919-1934.
  26. Yarger, J. G., C. A. Saunders, J. L. McNaughton, C. L. Quarles, B. W. Hollis, and R. W. Gray. 1995. Comparison of dietary 25-hydroxycholecalciferol and cholecalciferol in broiler chickens. Poult. Sci. 74:1159-1167.

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