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Association of lnc-LAMC2-1:1 rs2147578 and CASC8 rs10505477 Polymorphisms with Risk of Childhood Acute Lymphoblastic Leukemia

  • Hashemi, Mohammad (Cellular and Molecular Research Center, Zahedan University of Medical Sciences) ;
  • Bahari, Gholamreza (Cellular and Molecular Research Center, Zahedan University of Medical Sciences) ;
  • Naderi, Majid (Department of Pediatrics, School of Medicine, Zahedan University of Medical Sciences) ;
  • Bojd, Simin Sadeghi (Department of Pediatrics, School of Medicine, Zahedan University of Medical Sciences) ;
  • Taheri, Mohsen (Genetics of Non-Communicable Disease Research Center, Zahedan University of Medical Sciences)
  • Published : 2016.11.01

Abstract

Long non-coding RNAs (lncRNAs) are a novel class of non-protein coding RNAs that are involved in a wide variety of biological processes. There are limited data regarding the impact of lnc-LAMC2-1:1 rs2147578 as well as CASC8 rs10505477 T>C polymorphisms on cancer development. Here we examined for the first time whether rs2147578 and rs10505477 polymorphisms are associated with childhood acute lymphoblastic leukemia (ALL) in a total of 110 cases and 120 healthy controls. Genotyping was achieved by the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. The rs2147578 variant increased the risk of ALL in codominant (OR=4.33, 95%CI=2.00-9.37, p<0.0001, CG vs CC, and OR=5.81, 95%CI=2.30-14.69, p=0.0002, GG vs CC), dominant (OR=4.63, 95%CI=2.18-9.86, p<0.0001, CG+GG vs CC), overdominant (OR=1.74, 95%CI=1.02-2.97, p=0.0444, CG vs CC+GG) and allele (OR=1.91, 95%CI=1.32-2.77, p=0.0008, G vs C) inheritance models tested. No significant association was found between the CASC8 rs10505477 T>C variant and risk of childhood ALL. In conclusion, the present study revealed that the lnc-LAMC2-1:1 rs2147578 polymorphism may be a risk factor for developing childhood ALL. Further studies with larger sample sizes with different ethnicities are now required to confirm our findings.

Keywords

Long non-coding RNA;lnc-LAMC2-1:1;CASC8;acute lymphoblastic leukemia;Polymorphism

Acknowledgement

Supported by : Zahedan University of Medical Sciences

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