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Nano-Micelle of Moringa Oleifera Seed Oil Triggers Mitochondrial Cancer Cell Apoptosis

  • Abd-Rabou, Ahmed A (Hormones Department, Medical Research Division, National Research Centre) ;
  • Zoheir, Khairy M A (Cell Biology Department, National Research Centre) ;
  • Kishta, Mohamed S (Hormones Department, Medical Research Division, National Research Centre) ;
  • Shalby, Aziza B (Hormones Department, Medical Research Division, National Research Centre) ;
  • Ezzo, Mohamed I (Horticulture Technology Department, National Research Centre)
  • Published : 2016.11.01

Abstract

Cancer, a worldwide epidemic disease with diverse origins, involves abnormal cell growth with the potential to invade other parts of the body. Globally, it is the main cause of mortality and morbidity. To overcome the drawbacks of the commercially available chemotherapies, natural products-loaded nano-composites are recommended to improve cancer targetability and decrease the harmful impact on normal cells. This study aimed at exploring the anti-cancer impacts of Moringa oleifera seed oil in its free- (MO) and nano-formulations (MOn) through studying whether it mechanistically promotes mitochondrial apoptosis-mediating cell death. Mitochondrial-based cytotoxicity and flow cytometric-based apoptosis analyses were performed on cancer HepG2, MCF7, HCT 116, and Caco-2 cell lines against normal kidney BHK-21 cell line. The present study resulted that MOn triggered colorectal cancer Caco-2 and HCT 116 cytotoxicity via mitochondrial dysfunction more powerful than its free counterpart (MO). On the other side, MOn and MO remarkably induces HCT 116 mitochondrial apoptosis, while sparing normal BHK-21 cells with minimal cytotoxic effect. The present results concluded that nano-micelle of Moringa oleifera seed oil (MOn) can provide a novel therapeutic approach for colorectal and breast cancers via mitochondrial-mediated apoptosis, while sparing normal and even liver cancer cells a bit healthy or with minimal harmful effect. Intriguingly, MOn induced breast cancer not hepatocellular carcinoma cell death.

Keywords

Moringa oleifera;seed oil;Nano-micelle;mitochondrial apoptosis;cancer

Acknowledgement

Grant : Recent approaches in the utilization of Moringa oleifera and Moringa peregrina as a good nutritional, medicinal and industrial plant in Egypt

Supported by : Science and Technology Development Fund (STDF)

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