Evaluating the Existence of Small Compressed Binucleated Squamous Cells in ASC-H

  • Okodo, Mitsuaki (Department of Medical Technology, Kyorin University, Faculty of Health Sciences) ;
  • Okayama, Kaori (School of Medical Technology, Faculty of Health Science, Gumma Paz College) ;
  • Kitamura, Hiroshi (Department of Medical Oncology, Kyorin University School of Medicine) ;
  • Shiina, Natsuko (ILABO Cyto STD Laboratory, Inc) ;
  • Caniz, Timothy (ILABO Cyto STD Laboratory, Inc) ;
  • Ono, Midori (ILABO Cyto STD Laboratory, Inc) ;
  • Yabusaki, Hiromi (ILABO Cyto STD Laboratory, Inc)
  • Published : 2016.10.01


Purpose: To evaluate the legitimacy of a diagnosis of ASC-H in 5 cases which were followed up monthly for over 2 years with both cytology and HPV testing. Methods: Some 5 cases out of a total of 25.0 self-sampled Pap test patients diagnosed as ASC-H provided 119 specimens over 2 years, with HPV-DNA testing perormed using a E6 primer. Results: Cases 1, 2 and 3 showed SIL after the ASC-H diagnosis, while cases 4 and 5 showed and maintained NILM. Cases 1, 2 and 3 were further characterized by small atypical compressed binucleated cells, in which HPV was detected by in situ PCR. Case 4 showed a high N/C ratio in cells in sheets with a mild increase in chromatin. Case 5 demonstrated a high N/C ratio in small cells with no increase in chromatin. Conclusion: The finding of a compressed binucleated cells can define the difference between degenerated endocervical columnar cells and small atypical cells suggestive of HSIL. When small compressed binucleated squamous cells are detected, there may be a chance of continuing HPV infection and undetected SIL.


HPV;ASC-H;compressed binucleated squamous cells


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