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Lack of TNF-α Gene Polymorphism (rs1799724) Association with Sustained Virological Response in Iranian Patients with Chronic HCV Infection

  • Larijani, Mona Sadat (Department of Hepatitis and AIDS, Pasteur Institute of Iran) ;
  • Bahiraei, Narges (Islamic Azad University, Varamin Branch) ;
  • Nikbin, Mehri (Iran Hepatitis Network, Pasteur Institute of Iran) ;
  • Mohajel, Nasir (Department of Virology, Pasteur Institute of Iran) ;
  • Rad, Leila Naghizadeh (Department of Hepatitis and AIDS, Pasteur Institute of Iran) ;
  • Baghbani, Fahimeh (Islamic Azad University, Varamin Branch) ;
  • Mapar, Maryam (Department of Hepatitis and AIDS, Pasteur Institute of Iran) ;
  • Sadat, Seyed Mehdi (Department of Hepatitis and AIDS, Pasteur Institute of Iran)
  • Published : 2016.08.01

Abstract

Infection with the hepatitis C virus is a major public health concern which can lead to carcinoma and liver failure. It has been shown that single nucleotide polymorphisms can affect the level of gene activity of tumor necrosis factor (TNF) which has an important role, especially in viral infections which can lead to apaptosis of infected hepatocellular cells. We investigated the impact of three possible genotypes for rs1800629 or A/G single nucleotide polymorphism located downstream of $TNF{\alpha}$ gene promoter in groups of control (n=76) and chronic hepatitis C patients (n=89) focusing on the response to treatment among sensitive and resistant groups. Genomic DNA was extracted from $500{\mu}l$ prepheral whole blood and PCR and RFLP were used to amplify the region of interest and genotyping. With statistical analyzes a p-value <0.05 was considered meaningful. There was no significant difference in distribution of possible three genotypes among healthy individuals and patients (P=0.906, OR=1.194, CI=0.063-22.790). However, the frequency of G allele was higher in patients whereas A allele was more common among healthy individuals (p<0.0001). Further studies with more samples seem to be necessary.

Keywords

Hepatitis C;sustained virological respoanse;tumor necrosis factor-alpha;polymorphism, rs1800629;Iran

Acknowledgement

Supported by : Pasteur Institute of Iran

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