- Volume 17 Issue 8
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Preparation, Characterization and Cytotoxicity of Silibinin-Containing Nanoniosomes in T47D Human Breast Carcinoma Cells
- Amiri, Boshra (Chemistry faculty, Islamic Azad University of Shahrood) ;
- Ebrahimi-Far, Meysam (Department of Toxicology, Faculty of Pharmacy, Islamic Azad University) ;
- Saffari, Zahra (Pilot Nanobiotechnology Dep., Pasteur Institute of Iran) ;
- Akbarzadeh, Azim (Pilot Nanobiotechnology Dep., Pasteur Institute of Iran) ;
- Soleimani, Esmaeil (Chemistry faculty, Islamic Azad University of Shahrood) ;
- Chiani, Mohsen (Pilot Nanobiotechnology Dep., Pasteur Institute of Iran)
- Published : 2016.08.01
Background: Breast cancer is one of the most frequent cancer types within female populations. Silibinin is a chemotherapeutic agent ative against cancer. Niosomes are biodegradable, biocompatible, safe and effective carriers for drug delivery. Objective:To prepare nanoniosomal silibinin and evaluate its cytotoxicity inthe T-47D breast cancer cell line. Materials and Methods: Niosomes were prepared by reverse phase evaporation of a mixture of span 20, silibinin, PEG-2000 and cholesterol in chloroform and methanol solvent (1:2 v/v). The solvent phase was evaporated using a rotary evaporator and the remaining gel phase was hydrated in phosphate buffer saline. Mean size, size distribution and zeta potential of niosomes were measured with a Zetasizer instrument and then nanoparticles underwent scanning electron microscopy. The drug releasing pattern was evaluated by dialysis and the cytotoxicity of nanoniosomes in T-47D cells was assessed by MTT assay. Results: Particle size, size variation and zeta potential of the niosomal nanoparticles were measured as
Silibinin;nanoniosomes;breast cancer;T-47D cells
Supported by : Pilot Nanobiotechnology Department, Pasteur Institute of Iran
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