Detection of Human Papillomavirus among Women with Atypical Squamous Cells of Undetermined Significance Referred to Colposcopy: Implications for Clinical Management in Low- and Middle-Income Countries

  • de Abreu, Andre LP (Clinical Cytology Laboratory, Department of Clinical Analysis and Biomedicine, State University of Maringa) ;
  • Gimenes, Fabricia (Clinical Cytology Laboratory, Department of Clinical Analysis and Biomedicine, State University of Maringa) ;
  • Malaguti, Natalia (Clinical Cytology Laboratory, Department of Clinical Analysis and Biomedicine, State University of Maringa) ;
  • Pereira, Monalisa W (Clinical Cytology Laboratory, Department of Clinical Analysis and Biomedicine, State University of Maringa) ;
  • Uchimura, Nelson S (Department of Medicine, State University of Maringa) ;
  • Consolaro, Marcia EL (Clinical Cytology Laboratory, Department of Clinical Analysis and Biomedicine, State University of Maringa)
  • Published : 2016.07.01


To determine the prevalence of human papillomavirus (HPV) among women with atypical squamous cells of undetermined significance (ASC-US) referred to colposcopy and the implications for clinical management in low- and middle-income countries (LMIC), the present study was conducted. We included 200 women living in $Maring{\acute{a}}$/Brazil referred to colposcopy service between August 2012 and March 2013 due to an abnormal cytology from ASC-US until high-grade intraepithelial lesion (HSIL). HPV was detected and genotyped by polymerase chain reaction (PCR). The mean age was $36.8{\pm}10.5$ years, and women with and without ASC-US had similar mean ages ($37.4{\pm}11.5$ and $36.4{\pm}9.96$ years, respectively). The highest prevalence of ASC-US occurred at 20-24 years (40%). HPV-DNA was positive in 164 (82.0%) women.Of the 57 women with ASC-US, 30 (52.6%) were HPV-DNA-positive and 21 (70%) were high-risk HPV-positive (HR-HPV); the latter was similar to women without ASC-US (76.9%) but with other abnormal cytological findings present. Our data demonstrated that performing tests for HR-HPV can be used for management of women with ASC-US to support the decision of which women should be referred for an immediate or later colposcopy. The same conclusions can be applied to other LMICs for which HPV testing for primary screening has not been adopted.


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