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Lack of Effects of Recombinant Human Bone Morphogenetic Protein-2 on Angiogenesis in Oral Squamous Cell Carcinoma Induced in the Syrian hamster Cheek Pouch

  • Zaid, Khaled Waleed (Department of Oral Histology and Pathology, Faculty of Dentistry, University of Damascus) ;
  • Nhar, Bander Mossa (Al -Farabi Colleges Hospital) ;
  • Alanazi, Salman Mohammed Ghadeer (Al -Farabi Colleges Hospital) ;
  • Murad, Rashad (Faculty of Pharmacy, University of Damascus) ;
  • Domani, Ahmad (Al -Farabi Colleges Hospital) ;
  • Alhaf, Awadh Jamman (Al -Farabi Colleges Hospital)
  • Published : 2016.07.01

Abstract

Recombinant human bone morphogenetic protein-2 (rhBMP-2 ), a member of the TGF-${\beta}$ family, has been used widely in recent years to regenerate defects of the maxillary and mandible bones. Such defects are sometimes caused by resection of oral squamous cell carcinoma (OSCC) yet the biologic effects of rhBMP-2 on these carcinomas are not fully clear. The objective of this study was to determine histologically whether rhBMP-2 produces adverse effects on angiogenesis during induction of OSCC, a biologic process critical for tumor formation in an experimental model in the buccal pouch of golden Syrian hamsters. Buccal cavities were exposed to painting with 0.5% DMBA in liquid paraffin three times a week for 14 weeks, then biopsies were taken. Division was into 2 groups: a study group of 10 hamsters receiving $0.25{\mu}g/ml$ of rhBMP-2 in the $3^{rd}$ and $6^{th}$ weeks; and a control group of 10 hamsters which did not receive any additional treatment. VEGF expression and microvessel density were measured but no differences were noted between the two groups. According to this study, rh-BMP-2 does not stimulate angiogenesis during induction of OCSSs.

Keywords

Recombinant human bone morphogenetic protein-2;oral squamous cell carcinoma;angiogenesis

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