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Gene Expression of CYP1A1 and its Possible Clinical Application in Thyroid Cancer Cases

  • Gallegos-Vargas, JA (Service of Otolaryngology and Head and Neck Surgery, Hospital Central Militar, Secretaria de la Defensa Nacional) ;
  • Sanchez-Roldan, J (Service of Otolaryngology and Head and Neck Surgery, Hospital Central Militar, Secretaria de la Defensa Nacional) ;
  • Ronquillo-Sanchez, MD (Section of Graduate Studies and Research, Escuela Superior de Medicina, Instituto Politecnico Nacional) ;
  • Carmona-Aparicio, L (Laboratory of Neurosciences (Neurochemistry), Instituto Nacional de Pediatria) ;
  • Floriano-Sanchez, E (Laboratory of Molecular Biology, Escuela Militar de Graduados de Sanidad, Secretaria de la Defensa Nacional) ;
  • Cardenas-Rodriguez, N (Laboratory of Neurosciences (Neurochemistry), Instituto Nacional de Pediatria)
  • Published : 2016.07.01

Abstract

Background: Thyroid cancer is the most common endocrine malignancy, and exact causes remain unknown. The role of CYP450 1A1 (CYP1A1) in cancer initiation and progression has been investigated. The aim of this work was to analyze, for the first time, CYP1A1 gene expression and its relationship with several clinicopathological factors in Mexican patients diagnosed with thyroid cancer. Materials and Methods: Real-time PCR analysis was conducted on 32 sets of thyroid tumors and benign pathologies. Expression levels were tested for correlations with clinical and pathological data. All statistical analysis were performed using GraphPad Prism version 3.0 software. Results: We found that female gender was associated with thyroid cancer risk (P<0.05). A positive relationship was identified between CYP1A1 mRNA levels and the presence of chronic disease, alcohol use, tumor size, metastasis and an advanced clinical stage (P<0.05). Conclusions: The results suggest that CYP1A1 gene expression could be used as a marker for thyroid cancer.

Keywords

Thyroid cancer;cytochrome P450 1A1;molecular marker

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