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Expression of Epstein Barr Virus Encoded EBNA1 and LMP1 Oncoproteins in Nasopharyngeal Carcinomas from Northeast India

  • Borthakur, Parikhit (Department of Bioengineering and Technology, GU IST, Gauhati University) ;
  • Kataki, Kangkana (Department of Bioengineering and Technology, GU IST, Gauhati University) ;
  • Keppen, Chenole (Healthcare Laboratory and Research Centre, Naga Hospital Authority) ;
  • Khamo, V. (Healthcare Laboratory and Research Centre, Naga Hospital Authority) ;
  • Medhi, Subhash (Department of Bioengineering and Technology, GU IST, Gauhati University) ;
  • Deka, Manab (Department of Bioengineering and Technology, GU IST, Gauhati University)
  • Published : 2016.07.01

Abstract

Background: Nasopharyngeal carcinoma (NPC), a malignancy arising from the epithelial lining of the nasopharynx, is distinct from others cancers in terms of its epidemiologic features. It is rare in most parts of the world except for a few regions with populations of Mongoloid origin. Objectives: To study the expression pattern of Epstein Barr virus (EBV) encoded oncoproteins EBNA1 and LMP1 in different histological types of NPC and to correlate expression patterns with sex, age and histological types. Materials and Methods: A total of 40 formalin-fixed, paraffin-embedded NPC biopsy samples and tissues from 20 healthy controls were collected to study the expression level of EBNA1 and LMP1 using immunohistochemistry. Results: EBNA1 and LMP1 expression was found in 92.5% and 90% respectively, of the cases and none of the control specimens. The expression patterns of EBNA1 and LMP1 were determined to be statistically significant (p<0.05) when correlated with sex, age and histological distributions. Also immunohistochemistry was found to be a sensitive technique in the detection of EBV. Conclusions: The study reveals that the potent oncoproteins EBNA1 and LMP1 were over expressed in our population cohort. Our findings are to some extent inconsistent with earlier reports as our population showed a higher expression of both EBNA1 and LMP1 compared to other studies.

Keywords

Nasopharyngeal carcinoma;Epstein Barr virus;LMP1;EBNA1;North East India

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