Identification of a Novel Cassette Array in Integron-bearing Helicobacter Pylori Strains Isolated from Iranian Patients

  • Goudarzi, Mehdi (Department of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences) ;
  • Seyedjavadi, Sima Sadat (Department of Medical Mycology, Pasteur institute of Iran) ;
  • Fazeli, Maryam (WHO collaborating center for reference and research on rabies, Pasteur Institute of Iran) ;
  • Roshani, Maryam (Department of Biology, Faculty of Science, University Putra Malaysia) ;
  • Azad, Mehdi (Department of Medical Laboratory Sciences, School of Paramedicine, Qazvin University of Medical Sciences) ;
  • Heidary, Mohsen (Department of Medical Microbiology, Faculty of Medicine, Iran University of Medical Sciences) ;
  • Navidinia, Masoumeh (Department of Medical Laboratory Sciences, School of Paramedicine, Shahid Beheshti University of Medical Science) ;
  • Goudarzi, Hossein (Department of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences)
  • Published : 2016.07.01


Helicobacter pylori as the second most common cause of gastric cancer in the world infects approximately half of the developed countries population and 80% of the population living in developing countries. Integrons as genetic reservoirs play major roles in dissemination of antimicrobial resistance genes. To the best of our knowledge, this is the first study to report carriage of class 1 and 2 integrons and associated gene cassettes in H. pylori isolates from Iran. This cross-sectional study was conducted in Tehran among 110 patients with H. pylori infection. Antimicrobial susceptibility testing (AST) for H. pylori strains were assessed by the micro broth dilution method. Class 1 and 2 integrons were detected using PCR. In order to determine gene cassettes, amplified fragments were subjected to DNA sequencing of both amplicon strands. The prevalence of resistance to clarithromycin, metronidazole, clarithromycin, tetracycline, amoxicillin, rifampin, and levofloxacin were 68.2% (n=75), 25.5% (n=28), 24.5% (n=27), 19.1% (n=21), 18.2% (n=20) and 16.4% (n=18), respectively. Frequency of multidrug resistance among H. pylori isolates was 12.7%. Class 2 integron was detected in 50 (45.5%) and class 1 integron in 10 (9.1%) H. pylori isolates. The most predominant gene cassette arrays in class 2 integron-bearing H. pylori were included sat-era-aadA1, dfrA1-sat2-aadA1, blaoxa2 and, aadB whereas common gene cassette arrays in class 1 integron were aadB-aadA1-cmlA6, aacA4, blaoxa2, and catB3. The high frequency of class 2 integron and multidrug resistance in the present study should be considered as a warning for clinicians that continuous surveillance is necessary to prevent the further spread of resistant isolates.


Supported by : Shahid Beheshti University of Medical Sciences


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