- Volume 17 Issue 6
Application of Immunophenotyping and Heteroduplex Polymerase Chain Reaction (hPARR) for Diagnosis of Canine Lymphomas
- Sirivisoot, Sirintra (Department of Pathology, Faculty of Veterinary Science, Chulalongkorn University) ;
- Techangamsuwan, Somporn (Department of Pathology, Faculty of Veterinary Science, Chulalongkorn University) ;
- Tangkawattana, Sirikachorn (Department of Veterinary Pathobiology, Faculty of Veterinary Medicine, Khon Kaen University) ;
- Rungsipipat, Anudep (Department of Pathology, Faculty of Veterinary Science, Chulalongkorn University)
- Published : 2016.06.01
Background: Canine malignant lymphoma is classified into B- or T-cell origin, as in the human case. Due to differences in prognosis, a suitable method needs to be developed for lineage identification. Aims: To determine the accuracy of immunophenotypic and molecular information between three methods: immunocytochemistry (ICC), immunohistochemistry (IHC) and heteroduplex polymerase chain reaction for antigen receptor rearrangements (hPARR) in spontaneous canine lymphomas. Materials and Methods: Peripheral blood, fine needle aspiration and tissue biopsies from enlarged peripheral lymph nodes prior to treatment of 28 multicentric lymphoma patients were collected. Cytopathology and histopathology were examined and classified using the updated Kiel and WHO classifications, respectively. Anti-Pax5 and anti-CD3 antibodies as B- and T-cell markers were applied for immunophenotyping by ICC and IHC. Neoplastic lymphocytes from lymph node and white blood cell pellets from peripheral blood were evaluated by hPARR. Results: In this study, low grade B-cell lymphoma accounted for 25% (7/28), high grade B-cell lymphoma for 64.3% (18/28) and high grade T-cell lymphoma for 10.7% (3/28). According to the WHO classification, 50% of all cases were classified as diffuse large B-cell lymphoma. In addition, ICC showed concordant results with IHC; all B-cell lymphomas showed Pax5+/CD3, and all T-cell lymphomas exhibited Pax5-/CD3+. In contrast to hPARR, 12 B-cell lymphomas featured the IgH gene; seven presented the
Canine lymphoma;diagnostic methods;hPARR;ICC;IHC
Supported by : Chulalongkorn University
- Aresu L, Arico A, Ferraresso S, et al (2014). Minimal residual disease detection by flow cytometry and PARR in lymph node, peripheral blood and bone marrow, following treatment of dogs with diffuse large B-cell lymphoma. Vet J, 200, 318-24. https://doi.org/10.1016/j.tvjl.2014.03.006
- Aulbach AD, Swenson CL, Kiupel M (2010). Optimized processing of fine-needle lymph node biopsies for automated immunostaining. J Vet Diagn Invest, 22, 383-8. https://doi.org/10.1177/104063871002200306
- Boone E, Verhaaf B, Langerak AW (2013). PCR-based analysis of rearranged immunoglobulin or T-cell receptor genes by GeneScan analysis or heteroduplex analysis for clonality assessment in lymphoma diagnostics. Methods Mol Biol, 971, 65-91.
- Burnett RC, Vernau W, Modiano JF, et al (2003). Diagnosis of canine lymphoid neoplasia using clonal rearrangements of antigen receptor genes. Vet Pathol, 40, 32-41. https://doi.org/10.1354/vp.40-1-32
- Calzolari C, Gentilini F, Agnoli C, et al (2006). PCR assessment of minimal residual disease in 8 lymphoma-affected dogs. Vet Res Comm, 30, 285-8.
- Fernandes NC, Guerra JM, Ressio RA, et al (2015). Liquidbased cytology and cell block immunocytochemistry in veterinary medicine: comparison with standard cytology for the evaluation of canine lymphoid samples. Vet Comp Oncol.
- Fournel-Fleury C, Magnol JP, Bricaire P, et al (1997). Cytohistological and immunological classification of canine malignant lymphomas: comparison with human non-Hodgkin's lymphomas. J Comp Pathol, 117, 35-59. https://doi.org/10.1016/S0021-9975(97)80065-5
- Fournel-Fleury C, Ponce F, Felman P, et al (2002). Canine T-cell lymphomas: a morphological, immunological, and clinical study of 46 new cases. Vet Pathol, 39, 92-109. https://doi.org/10.1354/vp.39-1-92
- Goto-Koshino Y, Mochizuki H, Sato M, et al (2015). Construction of a multicolor GeneScan analytical system to detect clonal rearrangements of immunoglobulin and T cell receptor genes in canine lymphoid tumors. Vet Immunol Immunopathol, 165, 81-7. https://doi.org/10.1016/j.vetimm.2015.03.005
- Guija de Arespacochaga A, Schwendenwein I, Weissenbock H (2007). Retrospective study of 82 cases of canine lymphoma in Austria based on the Working Formulation and immunophenotyping. J Comp Pathol, 136, 186-92. https://doi.org/10.1016/j.jcpa.2007.01.009
- Jeon S, Lee MJ, Park J, et al (2007). Fast molecular diagnostics of canine T-cell lymphoma by PCR and capillary gel electrophoresis with laser-induced fluorescence detector. J Chromatogr B Analyt Technol Biomed Life Sci, 854, 268-72. https://doi.org/10.1016/j.jchromb.2007.04.050
- Kaneko N, Tanimoto T, Morimoto M, et al (2009). Use of formalin-fixed paraffin-embedded tissue and single-strand conformation polymorphism analysis for polymerase chain reaction of antigen receptor rearrangements in dogs. J Vet Med Sci, 71, 535-8. https://doi.org/10.1292/jvms.71.535
- Keller SM, Keller BC, Grest P, et al (2007). Validation of Tissue Microarrays for Immunohistochemical Analyses of Canine Lymphomas. J Vet Diagn Invest, 19, 652-9. https://doi.org/10.1177/104063870701900606
- Keller SM, Moore PF (2012). A novel clonality assay for the assessment of canine T cell proliferations. Vet Immunol Immunopathol, 145, 410-9. https://doi.org/10.1016/j.vetimm.2011.12.019
- Kiupel M, Teske E, Bostock D (1999). Prognostic factors for treated canine malignant lymphoma. Vet Pathol, 36, 292-300. https://doi.org/10.1354/vp.36-4-292
- Lana SE, Jackson TL, Burnett RC, et al (2006). Utility of polymerase chain reaction for analysis of antigen receptor rearrangement in staging and predicting prognosis in dogs with lymphoma. J Vet Intern Med, 20, 329-34. https://doi.org/10.1111/j.1939-1676.2006.tb02864.x
- Manachai N, Techangamsuwan S, Lacharoje S, et al (2014). Detection of minimal residual disease (MRD) in canine lymphoma. Comp Clin Pathol, 23, 199-204. https://doi.org/10.1007/s00580-012-1597-0
- Ponce F, Marchal T, Magnol JP, et al (2010). A morphological study of 608 cases of canine malignant lymphoma in France with a focus on comparative similarities between canine and human lymphoma morphology. Vet Pathol, 47, 414-33. https://doi.org/10.1177/0300985810363902
- Sapierzynski R (2010). Practical aspects of immunocytochemistry in canine lymphomas. Pol J Vet Sci, 13, 661-8.
- Sapierzynski R, Dolka I, Fabisiak M (2012). High agreement of routine cytopathology and immunocytochemistry in canine lymphomas. Pol J Vet Sci, 15, 247-52.
- Sapierzynski R, Micun J, Jagielski D, et al (2010). Cytopathology of canine lymphomas (100 cases). Pol J Vet Sci, 13, 653-9.
- Sozmen M, Tasca S, Carli E, et al (2005). Use of fine needle aspirates and flow cytometry for the diagnosis, classification, and immunophenotyping of canine lymphomas. J Vet Diagn Invest, 17, 323-30. https://doi.org/10.1177/104063870501700404
- Takanosu M, Tadika T, Kobayashi T (2010). Heteroduplex polymerase chain reaction is essential for canine receptor rearrangement analysis. J Vet Diagn Invest, 22, 760-3. https://doi.org/10.1177/104063871002200518
- Tamura K, Yagihara H, Isotani M, et al (2006). Development of the polymerase chain reaction assay based on the canine genome database for detection of monoclonality in B cell lymphoma. Vet Immunol Immunopathol, 110, 163-7. https://doi.org/10.1016/j.vetimm.2005.10.009
- Thalheim L, Williams LE, Borst LB, et al (2013). Lymphoma immunophenotype of dogs determined by immunohistochemistry, flow cytometry, and polymerase chain reaction for antigen receptor rearrangements. J Vet Intern Med, 27, 1509-16. https://doi.org/10.1111/jvim.12185
- Thilakaratne DN, Mayer MN, MacDonald VS, et al (2010). Clonality and phenotyping of canine lymphomas before chemotherapy and during remission using polymerase chain reaction (PCR) on lymph node cytologic smears and peripheral blood. Can Vet J, 51, 79-84.
- Valli V, Peters E, Williams C, et al (2009). Optimizing methods in immunocytochemistry: one laboratory's experience. Vet Clin Pathol, 38, 261-9. https://doi.org/10.1111/j.1939-165X.2009.00119.x
- Valli VE, Kass PH, San Myint M, et al (2013). Canine lymphomas: association of classification type, disease stage, tumor subtype, mitotic rate, and treatment with survival. Vet Pathol, 50, 738-48. https://doi.org/10.1177/0300985813478210
- Valli VE, San Myint M, Barthel A, et al (2011). Classification of canine malignant lymphomas according to the World Health Organization criteria. Vet Pathol, 48, 198-211. https://doi.org/10.1177/0300985810379428
- Vezzali E, Parodi AL, Marcato PS, et al (2010). Histopathologic classification of 171 cases of canine and feline non-Hodgkin lymphoma according to the WHO. Vet Comp Oncol, 8, 38-49. https://doi.org/10.1111/j.1476-5829.2009.00201.x
- Wallace KA, Goldschmidt MH, Patel RT (2015). Converting fluid-based cytologic specimens to histologic specimens for immunohistochemistry. Vet Clin Pathol, 44, 303-9. https://doi.org/10.1111/vcp.12239
- Wilkerson MJ, Dolce K, Koopman T, et al (2005). Lineage differentiation of canine lymphoma/leukemias and aberrant expression of CD molecules. Vet Immunol Immunopathol, 106, 179-96. https://doi.org/10.1016/j.vetimm.2005.02.020
- Willmann M, Mullauer L, Guija de Arespacochaga A, et al (2009). Pax5 immunostaining in paraffin-embedded sections of canine non-Hodgkin lymphoma: a novel canine pan pre-Band B-cell marker. Vet Immunol Immunopathol, 128, 359-65. https://doi.org/10.1016/j.vetimm.2008.11.019
- Yagihara H, Tamura K, Isotani M, et al (2007). Genomic organization of the T-cell receptor gamma gene and PCR detection of its clonal rearrangement in canine T-cell lymphoma/leukemia. Vet Immunol Immunopathol, 115, 375-82. https://doi.org/10.1016/j.vetimm.2006.11.005