- Volume 17 Issue 6
Polymorphisms of XRCC1 and XRCC2 DNA Repair Genes and Interaction with Environmental Factors Influence the Risk of Nasopharyngeal Carcinoma in Northeast India
- Singh, Seram Anil (Department of Biotechnology, Assam University Silchar) ;
- Ghosh, Sankar Kumar (Department of Biotechnology, Assam University Silchar)
- Published : 2016.06.01
Multiple genetic and environmental factors have been reported to play key role in the development of nasopharyngeal carcinoma (NPC). Here, we investigated interactions of XRCC1 Arg399Gln and XRCC2 Arg188His polymorphisms and environmental factors in modulating susceptibility to NPC in Northeast India. One-hundred NPC patients, 90 first-degree relatives of patients and 120 controls were enrolled in the study. XRCC1 Arg399Gln and XRCC2 Arg188His polymorphisms were determined using PCR-RFLP, and the results were confirmed by DNA sequencing. Logistic regression (LR) and multifactor dimensionality reduction (MDR) approaches were applied for statistical analysis. The XRCC1 Gln/Gln genotype showed increased risk (OR=2.76; P<0.024) of NPC. However, individuals with both XRCC1 and XRCC2 polymorphic variants had 3.2 fold elevated risk (P<0.041). An enhanced risk of NPC was also observed in smoked meat (OR=4.07; P=0.004) and fermented fish consumers (OR=4.34, P=0.001), and tobacco-betel quid chewers (OR=7.00; P=0.0001) carrying XRCC1 polymorphic variants. However, smokers carrying defective XRCC1 gene showed the highest risk (OR = 7.47; P<0.0001). On MDR analysis, the best model for NPC risk was the five-factor model combination of XRCC1 variant genotype, fermented fish, smoked meat, smoking and chewing (CVC=10/10; TBA=0.636; P<0.0001); whereas in interaction entropy graphs, smoked meat and tobacco chewing showed synergistic interactions with XRCC1. These findings suggest that interaction of genetic and environmental factors might increase susceptibility to NPC in Northeast Indian populations.
Nasopharyngeal carcinoma;XRCC1;XRCC2;MDR analysis;first-degree relatives
- Bei JX, Jia WH, Zeng YX (2012). Familial and large-scale case-control studies identify genes associated with nasopharyngeal carcinoma. Semin Cancer Biol, 22, 96-106. https://doi.org/10.1016/j.semcancer.2012.01.012
- Cao Y, Miao XP, Huang MY, et al (2006). Polymorphisms of XRCC1 genes and risk of nasopharyngeal carcinoma in the Cantonese population. BMC Cancer, 6, 167. https://doi.org/10.1186/1471-2407-6-167
- Chang F, Hu T, Wang G (2006). [Relationship between CYP1A1 and GSTM1 genetic polymorphisms and lung cancer susceptibility in population of Inner Mongolia]. Zhongguo Fei Ai Za Zhi, 9, 413-7.
- Cho EY, Hildesheim A, Chen CJ, et al (2003). Nasopharyngeal carcinoma and genetic polymorphisms of DNA repair enzymes XRCC1 and hOGG1. Cancer Epidemiol Biomarkers Prev, 12, 1100-4.
- Choudhury JH, Choudhury B, Kundu S, et al (2014). Combined effect of tobacco and DNA repair genes polymorphisms of XRCC1 and XRCC2 influence high risk of head and neck squamous cell carcinoma in northeast Indian population. Med Oncol, 31, 67. https://doi.org/10.1007/s12032-014-0067-8
- Choudhury JH, Ghosh SK (2014). Gene-environment interaction and susceptibility in head and neck cancer patients and in their first-degree relatives: a study of Northeast Indian population. J Oral Pathol Med.
- Dianova, II, Sleeth KM, Allinson SL, et al (2004). XRCC1-DNA polymerase beta interaction is required for efficient base excision repair. Nucleic Acids Res, 32, 2550-5. https://doi.org/10.1093/nar/gkh567
- Flores-Obando RE, Gollin SM, Ragin CC (2010). Polymorphisms in DNA damage response genes and head and neck cancer risk. Biomarkers, 15, 379-99. https://doi.org/10.3109/13547501003797664
- Forat-Yazdi M, Gholi-Nataj M, Neamatzadeh H, et al (2015). Association of XRCC1 Arg399Gln Polymorphism with Colorectal Cancer Risk: A HuGE Meta Analysis of 35 Studies. Asian Pac J Cancer Prev, 16, 3285-91. https://doi.org/10.7314/APJCP.2015.16.8.3285
- Ghosh SK, Mondal R (2012). Quick diagnosis of female genital tuberculosis using multiplex fast polymerase chain reaction in Southern Assam, India. Int J Gynaecol Obstet, 118, 72-3. https://doi.org/10.1016/j.ijgo.2012.02.006
- Ghosh SK, Singh AS, Mondal R, et al (2014a). Dysfunction of mitochondria due to environmental carcinogens in nasopharyngeal carcinoma in the ethnic group of Northeast Indian population. Tumour Biol.
- Ghosh SK, Singh AS, Mondal R, et al (2014b). Dysfunction of mitochondria due to environmental carcinogens in nasopharyngeal carcinoma in the ethnic group of Northeast Indian population. Tumour Biol, 35, 6715-24. https://doi.org/10.1007/s13277-014-1897-x
- Guo S, Li X, Gao M, et al (2013). The relationship between XRCC1 and XRCC3 gene polymorphisms and lung cancer risk in northeastern Chinese. PLoS One, 8, 56213. https://doi.org/10.1371/journal.pone.0056213
- Gupta B, Johnson NW (2014). Systematic review and metaanalysis of association of smokeless tobacco and of betel quid without tobacco with incidence of oral cancer in South Asia and the Pacific. PLoS One, 9, 113385. https://doi.org/10.1371/journal.pone.0113385
- Hahn LW, Ritchie MD, Moore JH (2003). Multifactor dimensionality reduction software for detecting gene-gene and gene-environment interactions. Bioinformatics, 19, 376-82. https://doi.org/10.1093/bioinformatics/btf869
- Huang GL, Guo HQ, Yu CY, et al (2011). XRCC1 polymorphisms and risk of nasopharyngeal carcinoma: a meta-analysis. Asian Pac J Cancer Prev, 12, 2329-33.
- Huang M, Dinney CP, Lin X, et al (2007). High-order interactions among genetic variants in DNA base excision repair pathway genes and smoking in bladder cancer susceptibility. Cancer Epidemiol Biomarkers Prev, 16, 84-91. https://doi.org/10.1158/1055-9965.EPI-06-0712
- Jia WH, Luo XY, Feng BJ, et al (2010). Traditional Cantonese diet and nasopharyngeal carcinoma risk: a large-scale casecontrol study in Guangdong, China. BMC Cancer, 10, 446. https://doi.org/10.1186/1471-2407-10-446
- Jiao L, Hassan MM, Bondy ML, et al (2008). XRCC2 and XRCC3 gene polymorphism and risk of pancreatic cancer. Am J Gastroenterol, 103, 360-7. https://doi.org/10.1111/j.1572-0241.2007.01615.x
- Johnson RD, Liu N, Jasin M (1999). Mammalian XRCC2 promotes the repair of DNA double-strand breaks by homologous recombination. Nature, 401, 397-9.
- Kataki AC, Simons MJ, Das AK, et al (2011). Nasopharyngeal carcinoma in the Northeastern states of India. Chin J Cancer, 30, 106-13. https://doi.org/10.5732/cjc.010.10607
- Kocabas NA, Karahalil B (2006). XRCC1 Arg399Gln genetic polymorphism in a Turkish population. Int J Toxicol, 25, 419-22. https://doi.org/10.1080/10915810600870567
- Kumar A, Pant MC, Singh HS, et al (2012). Associated risk of XRCC1 and XPD cross talk and life style factors in progression of head and neck cancer in north Indian population. Mutat Res, 729, 24-34. https://doi.org/10.1016/j.mrfmmm.2011.09.001
- Laantri N, Jalbout M, Khyatti M, et al (2011). XRCC1 and hOGG1 genes and risk of nasopharyngeal carcinoma in North African countries. Mol Carcinog, 50, 732-7. https://doi.org/10.1002/mc.20754
- Lamerdin JE, Montgomery MA, Stilwagen SA, et al (1995). Genomic sequence comparison of the human and mouse XRCC1 DNA repair gene regions. Genomics, 25, 547-54. https://doi.org/10.1016/0888-7543(95)80056-R
- Lavender NA, Benford ML, VanCleave TT, et al (2009). Examination of polymorphic glutathione S-transferase (GST) genes, tobacco smoking and prostate cancer risk among men of African descent: a case-control study. BMC Cancer, 9, 397. https://doi.org/10.1186/1471-2407-9-397
- Li Q, Wang JM, Peng Y, et al (2013). Association of DNA base-excision repair XRCC1, OGG1 and APE1 gene polymorphisms with nasopharyngeal carcinoma susceptibility in a Chinese population. Asian Pac J Cancer Prev, 14, 5145-51. https://doi.org/10.7314/APJCP.2013.14.9.5145
- Malakar M, Devi KR, Phukan RK, et al (2014). CYP2E1 genetic polymorphism with dietary, tobacco, alcohol habits, H. pylori infection status and susceptibility to stomach cancer in Mizoram, India. Asian Pac J Cancer Prev, 15, 8815-22. https://doi.org/10.7314/APJCP.2014.15.20.8815
- Metsola K, Kataja V, Sillanpaa P, et al (2005). XRCC1 and XPD genetic polymorphisms, smoking and breast cancer risk in a Finnish case-control study. Breast Cancer Res, 7, 987-97. https://doi.org/10.1186/bcr1333
- Mondal R, Ghosh SK, Choudhury JH, et al (2013). Mitochondrial DNA copy number and risk of oral cancer: a report from Northeast India. PLoS One, 8, 57771. https://doi.org/10.1371/journal.pone.0057771
- Nagaraj NS, Beckers S, Mensah JK, et al (2006). Cigarette smoke condensate induces cytochromes P450 and aldo-keto reductases in oral cancer cells. Toxicol Lett, 165, 182-94. https://doi.org/10.1016/j.toxlet.2006.03.008
- Ruan HL, Xu FH, Liu WS, et al (2010). Alcohol and tea consumption in relation to the risk of nasopharyngeal carcinoma in Guangdong, China. Front Med China, 4, 448-56. https://doi.org/10.1007/s11684-010-0280-6
- Sabitha K, Reddy MV, Jamil K (2010). Smoking related risk involved in individuals carrying genetic variants of CYP1A1 gene in head and neck cancer. Cancer Epidemiol, 34, 587-92. https://doi.org/10.1016/j.canep.2010.05.002
- Saikia BJ, Phukan RK, Sharma SK, et al (2014). Interaction of XRCC1 and XPD gene polymorphisms with lifestyle and environmental factors regarding susceptibility to lung cancer in a high incidence population in North East India. Asian Pac J Cancer Prev, 15, 1993-9. https://doi.org/10.7314/APJCP.2014.15.5.1993
- Sarkar S, Nagabhushan M, Soman CS, et al (1989). Mutagenicity and carcinogenicity of smoked meat from Nagaland, a region of India prone to a high incidence of nasopharyngeal cancer. Carcinogenesis, 10, 733-6. https://doi.org/10.1093/carcin/10.4.733
- Shi Q, Wang LE, Bondy ML, et al (2004). Reduced DNA repair of benzo[a]pyrene diol epoxide-induced adducts and common XPD polymorphisms in breast cancer patients. Carcinogenesis, 25, 1695-700. https://doi.org/10.1093/carcin/bgh167
- Singh SA, Choudhury JH, Kapfo W, et al (2015). Influence of the CYP1A1 T3801C Polymorphism on Tobacco and Alcohol-Associated Head and Neck Cancer Susceptibility in Northeast India. Asian Pac J Cancer Prev, 16, 6953-61. https://doi.org/10.7314/APJCP.2015.16.16.6953
- Sterpone S, Mastellone V, Padua L, et al (2010). Single-nucleotide polymorphisms in BER and HRR genes, XRCC1 haplotypes and breast cancer risk in Caucasian women. J Cancer Res Clin Oncol, 136, 631-6. https://doi.org/10.1007/s00432-010-0791-1
- Talukdar FR, Ghosh SK, Laskar RS, et al (2013). Epigenetic, genetic and environmental interactions in esophageal squamous cell carcinoma from northeast India. PLoS One, 8, 60996. https://doi.org/10.1371/journal.pone.0060996
- Tsao SW, Yip YL, Tsang CM, et al (2014). Etiological factors of nasopharyngeal carcinoma. Oral Oncol, 50, 330-8. https://doi.org/10.1016/j.oraloncology.2014.02.006
- Uppal V, Mehndiratta M, Mohapatra D, et al (2014). XRCC-1 Gene Polymorphism (Arg399Gln) and Susceptibility to Development of Lung Cancer in Cohort of North Indian Population: A Pilot Study. J Clin Diagn Res, 8, CC17-20.
- Visuvanathan S, Chong PP, Yap YY, et al (2014). Distribution and haplotype associations of XPD Lys751Gln, XRCC1 Arg280His and XRCC1 Arg399Gln polymorphisms with nasopharyngeal carcinoma in the Malaysian population. Asian Pac J Cancer Prev, 15, 2747-51. https://doi.org/10.7314/APJCP.2014.15.6.2747
- Xue WQ, Qin HD, Ruan HL, et al (2013). Quantitative association of tobacco smoking with the risk of nasopharyngeal carcinoma: a comprehensive meta-analysis of studies conducted between 1979 and 2011. Am J Epidemiol, 178, 325-38. https://doi.org/10.1093/aje/kws479
- Yu HP, Zhang XY, Wang XL, et al (2004). DNA repair gene XRCC1 polymorphisms, smoking, and esophageal cancer risk. Cancer Detect Prev, 28, 194-9. https://doi.org/10.1016/j.cdp.2004.01.004
- Yu X, Liu J, Zhu H, et al (2014). Synergistic association of DNA repair relevant gene polymorphisms with the risk of coronary artery disease in northeastern Han Chinese. Thromb Res, 133, 229-34. https://doi.org/10.1016/j.thromres.2013.11.017