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Lack of Association between the MiR146a Polymorphism and Susceptibility to Thai Childhood Acute Lymphoblastic Leukemia

  • Chansing, Kochpinchon (Department of Biomedical Science, Faculty of Science, Rangsit University) ;
  • Pakakasama, Samart (Hematology Unit, Pediatric Departments, Faculty of Medicine, Ramathibodi Hospital) ;
  • Hongeng, Suradej (Hematology Unit, Pediatric Departments, Faculty of Medicine, Ramathibodi Hospital) ;
  • Thongmee, Acharawan (Microbiology unit, Department of Biomedical Science, Faculty of Science, Rangsit University) ;
  • Pongstaporn, Wanida (Pathobiology Unit, Department of Biomedical Science, Faculty of Science, Rangsit University)
  • Published : 2016.05.01

Abstract

Background: MiRNAs, small non coding RNAs, play a role in the regulation of hematopoiesis, with effects on cell growth, differentiation, and apoptosis. In addition, MiRNAs are thought to play an important role in tumorigenesis. The miR146a G>C polymorphism can lead to alteration of miR146 expression, which appears to be associated with development and progression of several cancers. This study aimed to investigate the association of the miRNA146a (rs2910164) G>C polymorphism and susceptibility to childhood acute lymphoblastic leukemia (ALL) and clinical outcomes. Materials and Methods: Totals of 100 childhood ALL patients and 200 healthy children were studied for miR146a polymorphisms using polymerase chain reaction-restriction fragment-length polymorphism (PCR-RFLP). Results: The frequency of the miR146a G allele in controls was 0.40 compared with 0.38 in ALL patients. There was no association between miRNA146a (rs2910164) G>C polymorphism and susceptibility to childhood ALL (OR=1.484, 95%CI=0.712-3.093, p=0.290). Moreover, the frequencies of miR146a (rs2910164) G>C polymorphism were not associated with demographic data and clinical outcomes in ALL cases. Conclusions: The miRNA146a polymorphism was not significantly associated with susceptibility to Thai childhood ALL or any clinico-pathological variables.

Keywords

Acute lymphoblastic leukemia;miRNA146a;polymorphism;susceptibility

Acknowledgement

Supported by : Research Institute of Rangsit University

References

  1. Bartel DP (2004). MicroRNA: genomics, biogenesis and function. Cell, 116, 281-97. https://doi.org/10.1016/S0092-8674(04)00045-5
  2. Caterine Labbaye, Ugo Testa (2012). The emerging role of MIR-146a in the control of hematopoiesis, immune function and cancer. J Hematol Oncol, 5, 1-10. https://doi.org/10.1186/1756-8722-5-1
  3. Cong Yue, Meilin Wang, Bo Ding, et al (2011). Polymorphism of the pre-miRNA146a is associated with risk of cervical cancer in a Chinese population. Gynecologic oncol, 122, 33-37. https://doi.org/10.1016/j.ygyno.2011.03.032
  4. Garcon R, Marcucci G, Croce CM (2010). Targeting microRNA in cancer: rational, strategies and challenges. Nat Rev Drug Discov, 6, 259-69.
  5. Hai Lian, Lei Wang, Jingmin Zhang (2012). Increased risk of breast cancer associated with CC genotype of hsamiRNA146a rs2910164 polymorphism in Europeans. Plos One, 7, 31615. https://doi.org/10.1371/journal.pone.0031615
  6. Hasani SS, Hashemi M, Eskandari-Nasab E, et al (2014). A function polymorphism in the miR-146a gene is associated with the risk of childhood acute lymphoblastic leukemia : a preliminary report. Tumor Biol, 35, 219-25. https://doi.org/10.1007/s13277-013-1027-1
  7. Hwang HW, Mendell JT (2006). MicroRNAs in cell proliferation, cell death and tumorigenesis. Br J Cancer, 94, 776-80. https://doi.org/10.1038/sj.bjc.6603023
  8. Hyo Sung Jeon, Yong Hoon Lee, Shin Yup Lee, et al. (2014). A common polymorphism in pre-microRNA-146a is associated with lung cancer risk in a Korean population. Gene, 543, 66-71.
  9. Inaba H, Greaves M, Mullighan CG (2013). Acute lymphoblastic leukemia. Lancet, 381, 1943-55. https://doi.org/10.1016/S0140-6736(12)62187-4
  10. Jazdzewski K, Murray EL, Franssila K, et al (2008).Common SNP in pre-miR-146a decreases mature miR expression and predisposes to papillary thyroid carcinoma. Proc Natl Acad Sci USA.105, 7269-74. https://doi.org/10.1073/pnas.0802682105
  11. Lili Yang,1 Mark P. Boldin,2 Yang Yu, et al (2012). miR-146a controls the resolution of T cell responses in mice. J Exp Med, 209,1655-70. https://doi.org/10.1084/jem.20112218
  12. Miller SA, Dykes DD, Polesky HF (1988). A simple salting out procedure for extracting DNA from human nucleated cell. Nucleic Acids Res, 16, 1215. https://doi.org/10.1093/nar/16.3.1215
  13. Min KT, Kim JW, Jeon YJ, et al (2012). Association of the miRNA146a C>G,149C>T, 196a2C>T and 499A>G polymorphism with colorectal cancer in the Korean population. Molecular Carcinogenesis, 51, 65-73. https://doi.org/10.1002/mc.21849
  14. Palmieri A, Carinci F, Martinelli M, et al (2014). Role of the MiR146a polymorphism in the origin and progression of oral squamous cell carcinoma. Eur J Oral Sci, 122, 198-201. https://doi.org/10.1111/eos.12121
  15. Pui CH, Evans WE. (2006). Acute lymphoblastic leukemia. N Engl J Med, 354, 166-78. https://doi.org/10.1056/NEJMra052603
  16. Qi P, Wang L, Zhou B, et al (2015). Associations of miRNA polymorphisms and expression levels with breast cancer risk in the Chinese population. Genet Mol Res, 14, 6289-96. https://doi.org/10.4238/2015.June.11.2
  17. Smith M, Arthur D, Canmitta B, Carroll AJ, et al (1996). Uniform approach to risk classification and treatment assignment for children with acute lymphoblastic leukemia. J Clin Onco, 14, 18-24. https://doi.org/10.1200/JCO.1996.14.1.18
  18. Taganov KD, Boldin MP, Chang KJ, Baltimore D (2006). NF-kappaB-dependent induction of microRNA miR-146, an inhibitor targeted to signaling proteins of innate immune responses. Proc Natl Acad Sci USA, 103, 12481-6. https://doi.org/10.1073/pnas.0605298103
  19. Tian T, Yongqian S, Jiaping C, et al (2009). A functional genetic variant in microRNA-196a2 is associated with increased susceptibility of lung cancer in Chinese. Cancer Epidemiol Biomarkers Prev, 18, 1183-7. https://doi.org/10.1158/1055-9965.EPI-08-0814
  20. Trevino LR, Yang W, French D, et al (2009). Germline genomic variants associated with childhood acute lymphoblastic leukemia. Nat Genet, 41, 1001-5. https://doi.org/10.1038/ng.432
  21. Vrooman LM, Silverman LB (2009). Childhood acute lymphoblastic leukemia: update on prognostic factors. Curr Opin Pediatr, 21, 1-8. https://doi.org/10.1097/MOP.0b013e32831f1f24
  22. Wang AX, Xu B, Tong N, et al (2012). Meta-analysis confirms that a common G/C variant in the pre-miR-146a gene contributes to cancer susceptibility and that ethnicity gender and smoking status are risk. Genetics Molecular Res, 11, 3051-62. https://doi.org/10.4238/2012.August.31.2
  23. Wei Du, Xue-Lei Ma, Chong Zhao, et al (2014). Associations of single nucleotide polymorphisms in miR-146a, miR-196a, miR-149 and miR-499 with colorectal cancer susceptibility. Asian Pac J Cancer Prev, 15, 1047-55. https://doi.org/10.7314/APJCP.2014.15.2.1047
  24. Yin Hung Chu, Shu Ling Tzegn, Chiao-Wen Lin, et al. (2012). Impacts of miRNA gene polymorphisms on the susceptibility of environmental factors leading to carcinogenesis in oral cancer. Plos One, 7, 39777. https://doi.org/10.1371/journal.pone.0039777
  25. Zhaoming Wang, Lei Zhang, Xuesong Shi, et al (2014). Association between two common polymorphisms and risk of hepatocellular carcinoma: evidence from an updated meta-analysis. BioMed Res Int, 2014.
  26. Zhong Xu, Lingling Zhang, Hui Cao, Banjun Bai (2014) MiR-146a rs2910164 G/C polymorphism and gastric cancer susceptibility: a meta-analysis. BMC Medical Genetics, 15, 117.