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Increased Serum Endoglin and Transforming Growth Factor β1 mRNA Expression and Risk of Hepatocellular Carcinoma in Cirrhotic Egyptian Patients

  • Teama, Salwa (Department of Clinical Pathology, National Cancer Institute, Cairo University) ;
  • Fawzy, Amal (Department of Molecular Biology, Faculty of Medicine, Ain Shams University) ;
  • Teama, Shirin (Department of Clinical Pathology, Faculty of Medicine, Ain Shams University) ;
  • Helal, Amany (Department of Medical Oncology/Hematology, National Cancer Institute, Cairo University) ;
  • Drwish, Amira Diyaa (Department of Medical Oncology/Hematology, National Cancer Institute, Cairo University) ;
  • Elbaz, Tamer (Department of Endemic Hepatogastroenterology, Cairo University) ;
  • Desouky, Eman (Department of Statistics, National Cancer Institute, Cairo University)
  • Published : 2016.05.01

Abstract

Transforming growth factor-B1 ($TGF-{\beta}1$ )and its coreceptor endoglin (ENG) have been shown to contribute to hepatocellular tumor development and malignant progression. Our aim was to evaluate the serum expression levels of $ENG/TGF-{\beta}1$ mRNAs and risk of hepatocellular carcinoma in cirrhotic Egyptian patients. Our study included 77 subjects. Real time polymerase chain reaction was used to evaluate the expression level of ENG and $TGF-{\beta}1$mRNAs. The relative expression ratio of ENG mRNA was 0.82 (0.1 -3.2), 0.66 (0.15-5.3), 0.38(0.007-2.8) and 0.12 (0.00-0.22) and the relative expression ratio of $TGF-{\beta}1$mRNA was 1.4 (0.19 -6.2), 1.2 (0.22-4.3), 1.0 (0.15-4.4) and 0.6 (0.00-2.2) for cirrhotic HCC cirrhotic, HCC only and healthy control groups respectively. Increased ENG and $TGF-{\beta}1$ mRNA gene expression was correlated with TNM clinical stage. The expression ratio in TNM stage III-IV 1.1 (0.07-3.2), 1.55 (0.15-6.2) was statistically significantly higher than that in stage I-II 0.47 (0.007-2.8), 1.0 (0.31-4.4) (P<0.05). Our data suggested that increased ENG and $TGF-{\beta}1$ gene expression may participate in hepatocarcinogenesis and increased risk of HCC in individuals with cirrhosis. Early screening for evidence of cirrhosis and consideration of ENG and $TGF-{\beta}1$ as targets for therapy and treatment strategies are warranted.

Keywords

Endoglin;$TGF{\beta}1$;mRNA;quantitative PCR;hepatocellular carcinoma

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