Detection of Epstein-Barr Virus and Cytomegalovirus in Gastric Cancers in Kerman, Iran

  • Leila, Zaruni (Department of Medical Microbiology, Kerman University of Medical Sciences) ;
  • Arabzadeh, Seyed Alimohammad (Department of Medical Microbiology, Kerman University of Medical Sciences) ;
  • Afshar, Reza Malekpour (Pathology and Stem Cell Research center, Kerman University of Medical Sciences) ;
  • Afshar, Abbas Aghaei (Leishmania Research Center, Kerman University of Medical Sciences) ;
  • Mollaei, Hamid Reza (Department of Medical Microbiology, Kerman University of Medical Sciences)
  • Published : 2016.05.01


Gastric cancer (GC) is a multifactorial disease with different factors having roles in its genesis. Helicobacter pylori and Epstein-Barr virus (EBV) are known infectious agents that could contribute. In addition, there is evidence of a relationship with cytomegalovirus (CMV). Since data on CMV prevalence in gastric cancer are limited, we here evaluated the frequency of EBV and CMV in Iranian patients. Ninety paraffin blocks of GC tissues from patients in Kerman were evaluated for the presence of EBV and CMV genomes by real-time polymerase chain reaction. EBV was detected in 10 cases (11.1%) and CMV in seven. One out of 17 female patients (5.88%) and nine out of 73 male patients (12.3%) were positive for EBV, while one out of 17 female patients (5.88%) and six out of 73 male patients (8.22%) were positive for CMV. The mean age for EBV-positive patients was $60.5{\pm}14.9years$ and the mean age for CMV-positive patients was $67.9{\pm}12.3years$. This study shows that the frequency of EBV-associated GC is high in Kerman. It also indicates that further studies of associations between GC and CMV are warranted, covering larger samples and populations from different areas of the world.


Gastric cancer;tissue samples;cytomegalovirus;Epstein-Barr virus;PCR detection


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