Effect of Luteinising Hormone Releasing Hormone Analogue and Human Chorionic Gonadotropin on Spermiation in the Tongue Sole, Cynoglossus semilaevis

Luteinising Hormone Releasing Hormone Analogue과 Human Chorionic Gonadotropin 처리가 박대(Cynoglossus semilaevis)의 배정에 미치는 영향

  • Received : 2016.03.21
  • Accepted : 2016.04.11
  • Published : 2016.04.30


Experiments were carried out examine short-term effects of human chorionic gonadotropin (hCG) and long-term effects of luteinising hormone releasing hormone analogue (LHRHa) on milt production of the tongue sole Cynoglossus semilaevis. In the first experiment, each fish was implanted with a blank cholesterol pellet (control), 100 and $200{\mu}g$ LHRHa per kg body weight. In the second experiment, fish were injected with either 100, 200, 400 and 800 IU hCG per kg body weight or same volume of marine fish Ringer's solution. In the first experiment, milt volume was increased in male implanted with $200{\mu}g$ LHRHa pellet compared with other groups at day 10. Injection of 400 and 800 IU hCG resulted in an increase in the milt volume at hour 96 after the treatment. Although statistical difference is unable to confirm because of small milt volume, compared with the control group, hormone pellet-treated groups had a reduction in the mean spermatocrit (Sct) and sperm concentration (Sc). The results suggest that the increase in milt volume is at least partially gonadotropin (GtH)-dependent and increased milt volume has a relationship with milt hydration.


Tongue sole;Cynoglossus semilaevis;spermiation;hormone


Supported by : 목포대학교


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