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Changes in Salivary Cortisol Concentration in Horses during Different Types of Exercise

  • Kang, Ok-Deuk (Department of Hippotherapy Welfare, SungDuk C.University) ;
  • Lee, Wang-Shik (Division of Biotechnology, Jeju National University)
  • Received : 2016.01.05
  • Accepted : 2016.02.29
  • Published : 2016.05.01

Abstract

This study aimed to estimate the change of stress level in horses based on cortisol concentration levels in their saliva. A total of 61 horses were divided into the following three groups: i) tourist riding experience (TR, n = 23); ii) resting group (RR, n = 14); and iii) horse-riding education (ER, n = 24). The saliva samples of TR and ER groups were taken using plain cotton Salivettes four times a day: at 07:00 (basal), 11:00 (Exercise 1, after 1-hour exercise in the morning), 14:00 (Exercise 2, after 1-hour exercise in the afternoon), and 16:00 (Exercise 3, after 1-hour exercise in the afternoon). The saliva samples of RR were measured at the same time. The samples were analyzed using the SAS program general linear model procedure. In a percentage relative to the base value, cortisol levels in Exercise 3 were confirmed to decrease in all groups as compared to the basal value percentage in the following sequence: ER>TR>RR. The highest peak was confirmed in Exercise 2 (approximately 131%) of RR group and the lowest peak appeared in Exercise 3 (approximately 52%) of ER group. Therefore, resting without any particular exercise can also increase the stress level of horses. Thus, it is better to exercise, as exercise can reduce the stress level, even in cases when riders are clumsy or lack appropriate horse-riding experience. The results of the present study are useful to equestrian center owners and educational riding instructors in that they provide a meaningful insight into a better horse management.

Keywords

Animal Welfare;Horse;Riding Experience;Stress;Salivary Cortisol

Acknowledgement

Supported by : Rural Development Administration

References

  1. Ferlazzo, A., P. Medica, C. Cravana, and E. Fazio. 2009. Endocrine changes after experimental showjumping. Comp. Exerc. Phys. 6:59-66. https://doi.org/10.1017/S1755254009990110
  2. Harewood, E. I. and C. M. McGowan. 2005. Behavioral and physiological responses to stabling in Naive Horses. J. Equine Vet. Sci. 4:164-170.
  3. Kang, O. D., Y. C. Ryu, C. C. Ryew, W. Y. Oh, C. E. Lee, and M. S. Kang. 2010. Comparative analyses of rider position according to skill levels during walk and trot in Jeju horse. Hum. Mov. Sci. 29:956-963. https://doi.org/10.1016/j.humov.2010.05.010
  4. Kędzierski, W., K. Strzelec, A. Cywinska, and S. Kowalik. 2013. Salivary cortisol concentration in exercised thoroughbred horses. J. Equine Vet. Sci. 33:1106-1109. https://doi.org/10.1016/j.jevs.2013.04.011
  5. Larsson, L., P. H. Pilborg, M. Jonansen, M. T. Christophersen, A. Holte, L. Roepstorff, L. H. Olsen, and A. P. Harrison. 2013. Physiological parameters of endurance horses pre compared basal values post-race, correlated with performance: A two race study from Scandinavia. ISRN Vet. Sci. Article, ID 684353: 12pages.
  6. Linden, A., T. Art, H. Amory, D. Desmecht, and P. Lekeux. 1991. Effect of 5 different types of exercise, transportation and ACTH administration of plasma cortisol concentration in sport horses. Equine Exerc. Physiol. 3:391-396.
  7. Malinowski, K., E. J. Shock, P. Rochelle, C. F. Kearns, P. D. Guirnalda, and K. H. McKeever. 2006. Plasma beta-endorphin, cortisol and immune responses to acute exercise are altered by age and exercise training in horses. Equine Vet. J. (Suppl.) 36:267-273.
  8. Marc, M., N. Parvizi, F. Ellendorff, E. Kallweit, and F. Elsaesser. 2000. Plasma cortisol and ACTH concentrations in the warmblood horse in response to a standardized treadmill exercise test as physiological markers for evaluation of training status. J. Anim. Sci. 78:1936-1946. https://doi.org/10.2527/2000.7871936x
  9. Moons, C., C. R. Heleski, C. M. Leece, and A. J. Zanella. 2002. Conflicting results in the association between plasma and salivary cortisol level in foals. Proceedings of the Dorothy Russel Havemeyer Foundation Workshop "Horse Behavior and Welfare". Holar College te Holar, Iceland.
  10. Mostl, E. and R. Palme. 2002. Hormones as indicators of stress. Domest. Anim. Endocrinol. 23:67-74. https://doi.org/10.1016/S0739-7240(02)00146-7
  11. Nogueira, G. P. and R. C. Barnabe. 1997. Is the Thoroughbred race-horse under chronic stress? Brazilian J. Med. Biol. Res. 30:1237-1239. https://doi.org/10.1590/S0100-879X1997001000016
  12. Peeters, M., C. Coline, J-F. Becker, D. Ledoux, and M. Vandenheede. 2011. Comparison between blood serum and salivary cortisol concentrations in horses using an adrenocorticotropic hormone challenge. Equine Vet. J. 43:487-493. https://doi.org/10.1111/j.2042-3306.2010.00294.x
  13. Peeters, M. M., C. C. Coline, J-F. Becker, and M. Vandenheede. 2013. Rider and horse salivary cortisol levels during competition and impact on performance. J. Equine. Vet. Sci. 33:155-160. https://doi.org/10.1016/j.jevs.2012.05.073
  14. Shanahan, S. 2003. Trailer loading stress in horses: Behavioral and physiological effects of nonaversive training (TTEAM). J. Appl. Anim. Welf. Sci. 6:263-274. https://doi.org/10.1207/s15327604jaws0604_1
  15. Strzelec, K., M. Kankofer, and S. Pietrzak. 2011. Cortisol concentration in the saliva of horses subjected to different kinds of exercise. Acta Vet. Brno 80:101-105. https://doi.org/10.2754/avb201180010101
  16. Strzelec, K., W. Kedzierski, A. Bereznowski, I. Janczarek, K. Bocian, and M. Racosz. 2013. Salivary cortisol Levels in horses and their riders during Three-day-events. Bull. Vet. Inst. Pulawy 57:237-241. https://doi.org/10.2478/bvip-2013-0042
  17. Van Der Kolk, J. H., R. F. Nachreiner, H. C. Scott, K. R. Refsal, and K. J. Zanella. 2001. Salivary and plasma concentration of cortisol in normal horses and horses with Cushing's disease. Equine Vet. J. 33:211-213.
  18. Vincze, A., C. S. Szabo, A. Heves, S. Veres, D. Uto, and L. Babinszky. 2010. Effect of age and event on post exercise values of blood biochemical parameters in show jumping horses. Acta Agraria KaposvAriensis 14:185-191.
  19. Wellhoener, P., J. Born, H. L. Fehm, and C. Dodt. 2004. Elevated resting and exercise-induced cortisol levels after mineralocorticoid receptor blockade with canrenoate in healthy humans. J. Clin. Endocrinol. Metab. 89:5048-5052. https://doi.org/10.1210/jc.2004-0086

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