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Development of a novel genetic assay for telomere recombination in Saccharomyces cerevisiae

효모에서 텔로미어 재조합을 관찰하기 위한 새로운 유전학적 연구방법의 개발

  • Kim, Min-Kyu (Department of Biological Sciences, College of Natural Science, Inha University) ;
  • Bae, Sung-Ho (Department of Biological Sciences, College of Natural Science, Inha University)
  • 김민규 (인하대학교 자연과학대학 생명과학과) ;
  • 배성호 (인하대학교 자연과학대학 생명과학과)
  • Received : 2015.12.23
  • Accepted : 2016.03.07
  • Published : 2016.03.31

Abstract

Stable maintenance of telomere is required for cell proliferation and survival. Although telomerase is the primary means for telomere maintenance, recombination is another important pathway to maintain telomeres. In this study, we developed a genetic assay for telomere recombination using the internal $TG_{1-3}$ repeats present in subtelomeric regions of yeast. The recombination frequencies were dependent on the presence of the internal $TG_{1-3}$ repeats. PCR amplification of the regions near URA3 and CAN1 markers using genomic DNA isolated from $FOA^rCan^r$ colonies indicated that each isolate had lost the chromosome end including the markers. In addition, the recombination frequencies increased with longer internal $TG_{1-3}$ repeats. Our results suggest that the $FOA^rCan^r$ colony formation is the consequence of recombination between the internal and terminal $TG_{1-3}$ repeats.

Keywords

assay for telomere recombination;telomere;telomere recombination;yeast telomere

Acknowledgement

Supported by : 한국연구재단

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