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Phylogenetic diversity and UV resistance analysis of radiation-resistant bacteria isolated from the water in Han River

한강물로부터 분리된 방사선 내성 세균들의 계통학적 다양성 및 UV 내성 분석

  • Lee, Jae-Jin (Department of Bio & Environmental Technology, College of Natural Science, Seoul Women's University) ;
  • Joo, Eun Sun (Department of Bio & Environmental Technology, College of Natural Science, Seoul Women's University) ;
  • Lee, Do Hee (Department of Bio & Environmental Technology, College of Natural Science, Seoul Women's University) ;
  • Jung, Hee-Young (College of Agricultural and Life Sciences, Kyungpook National University) ;
  • Kim, Myung Kyum (Department of Bio & Environmental Technology, College of Natural Science, Seoul Women's University)
  • 이재진 (서울여자대학교 자연과학대학 생명환경공학과) ;
  • 주은선 (서울여자대학교 자연과학대학 생명환경공학과) ;
  • 이도희 (서울여자대학교 자연과학대학 생명환경공학과) ;
  • 정희영 (경북대학교 농업생명과학대학 응용생명과학부) ;
  • 김명겸 (서울여자대학교 자연과학대학 생명환경공학과)
  • Received : 2016.03.04
  • Accepted : 2016.03.22
  • Published : 2016.03.31

Abstract

The aim of this study was to investigate the UV-resistance of radiation-resistant bacteria isolated from the water of Han River, South Korea. The water sample was irradiated with 3 kGy gamma radiation prior to isolation. Radiation-resistant bacterial strains were isolated by standard serial dilution method on R2A and 1/10 diluted R2A agar. The resulting purely isolated 60 cultures of bacteria were analysed for UV resistance and used in further studies. Based on the comparative analyses of 16S rRNA gene sequences, the bacterial isolates were divided into 3 phyla (4 genera): the phylum Deinococcus-Thermus (the genus Deinococcus) was 61.7%, Bacteroidetes (Hymenobacter and Spirosoma) was 23.4%, and Firmicutes (Exiguobacterium) was 15%. The results suggested that twenty-nine isolates are candidates new species belonging to Deinococcus, Hymenobacter, and Spirosoma, or other new genera. Nine bacterial strains were selected among the novel candidates and the UV-resistance analysis was conducted. All the candidate bacterial strains showed high UV resistance, similar to that of D. radiodurans R1.

Keywords

16S rDNA;Han River;phylogenetic tree;radiation-resistant bacteria;UV resistance analysis;water

Acknowledgement

Supported by : 서울여자대학교

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