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Succession of bacterial community structure during the early stage of biofilm development in the Antarctic marine environment

남극 해양에서 생물막 생성 초기 단계의 세균 군집 구조 변화

  • Lee, Yung Mi (Division of Polar Life Sciences, Korea Polar Research Institute) ;
  • Cho, Kyung Hee (Division of Polar Life Sciences, Korea Polar Research Institute) ;
  • Hwang, Kyuin (Division of Polar Life Sciences, Korea Polar Research Institute) ;
  • Kim, Eun Hye (Division of Polar Life Sciences, Korea Polar Research Institute) ;
  • Kim, Mincheol (Arctic Research Center, Korea Polar Research Institute) ;
  • Hong, Soon Gyu (Division of Polar Life Sciences, Korea Polar Research Institute) ;
  • Lee, Hong Kum (Division of Polar Life Sciences, Korea Polar Research Institute)
  • 이영미 (극지연구소 극지생명과학연구부) ;
  • 조경희 (극지연구소 극지생명과학연구부) ;
  • 황규인 (극지연구소 극지생명과학연구부) ;
  • 김은혜 (극지연구소 극지생명과학연구부) ;
  • 김민철 (극지연구소 북극환경자원연구센터) ;
  • 홍순규 (극지연구소 극지생명과학연구부) ;
  • 이홍금 (극지연구소 극지생명과학연구부)
  • Received : 2016.02.11
  • Accepted : 2016.03.11
  • Published : 2016.03.31

Abstract

Compared to planktonic bacterial populations, biofilms have distinct bacterial community structures and play important ecological roles in various aquatic environments. Despite their ecological importance in nature, bacterial community structure and its succession during biofilm development in the Antarctic marine environment have not been elucidated. In this study, the succession of bacterial community, particularly during the early stage of biofilm development, in the Antarctic marine environment was investigated by pyrosequencing of the 16S rRNA gene. Overall bacterial distribution in biofilms differed considerably from surrounding seawater. Relative abundance of Gammaproteobacteria and Bacteroidetes which accounted for 78.9-88.3% of bacterial community changed drastically during biofilm succession. Gammaproteobacteria became more abundant with proceeding succession (75.7% on day 4) and decreased to 46.1% on day 7. The relative abundance of Bacteroidetes showed opposite trend to Gammaproteobacteria, decreasing from the early days to the intermediate days and becoming more abundant in the later days. There were striking differences in the composition of major OTUs (${\geq}1%$) among samples during the early stages of biofilm formation. Gammaproteobacterial species increased until day 4, while members of Bacteroidetes, the most dominant group on day 1, decreased until day 4 and then increased again. Interestingly, Pseudoalteromonas prydzensis was predominant, accounting for up to 67.4% of the biofilm bacterial community and indicating its important roles in the biofilm development.

Acknowledgement

Supported by : Korea Polar Research Institute

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