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Gibberellin A7 production by Aspergillus tubingensis YH103 and cultural characteristics of endophytic fungi isolated from Tetragonia tetragonoides in Dokdo islands

독도 번행초에서 분리된 내생균류의 배양적 특성과 Aspergillus tubingensis YH103의 gibberellin A7의 생산

  • You, Young-Hyun (Marine Microorganism Team, National Marine Biodiversity Institute of Korea) ;
  • Park, Jong Myong (School of Life Science, Kyungpook National University) ;
  • Lim, Sung Hwan (Freshwater Biodiversity Research Division, Nakdonggang National Institute of Biological Resources) ;
  • Kang, Sang-Mo (School of Applied Biosciences, Kyungpook National University) ;
  • Park, Jong-Han (Horticultural & Herbal Crop Environment Division, National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Lee, In-Jung (School of Applied Biosciences, Kyungpook National University) ;
  • Kim, Jong-Guk (School of Life Science, Kyungpook National University)
  • 유영현 (국립해양생물자원관 해양미생물팀) ;
  • 박종명 (경북대학교 생명과학부) ;
  • 임성환 (국립낙동강생물자원관 담수생물다양성연구실) ;
  • 강상모 (경북대학교 응용생명과학부) ;
  • 박종한 (농촌진흥청 국립원예특작과학원 원예특작환경과) ;
  • 이인중 (경북대학교 응용생명과학부) ;
  • 김종국 (경북대학교 생명과학부)
  • Received : 2015.12.28
  • Accepted : 2016.02.03
  • Published : 2016.03.31

Abstract

Coastal plant species Tetragonia tetragonoides (Pall.) Kuntze native to the Dokdo islands was sampled and then 17 endophytic fungi were purely isolated based on morphological differences. The fungal isolates were characterized by their growth properties under NaCl concentration or pH gradient. Culture filtrates of the 17 fungal isolates were treated to Waito-c rice (WR) seedlings for verifying plant growth-promoting activity. As the results, YH103 strain showed the highest plant growth-promoting activity among them. Phylogenetic analysis of the isolates was done by the maximum likelihood method based on partial internal transcribed spacer region (ITS region: contaning ITS1, 5.8S, and ITS2), beta-tubulin (BenA), and calmodulin (CaM) gene sequences. Chromatographic analysis of the strain YH103 culture filtrate showed the existence of gibberellins ($GA_4$, $GA_7$, $GA_8$, and $GA_{19}$). Finally, the strain YH103 was identified as Aspergillus tubingensis by microscopic observation and molecular analysis and, to our knowledge, this is the first report of GAs producing A. tubingensis.

Keywords

Aspergillus tubingensis;endophytic fungi;gibberellin A7;plant growth-promoting activity

Acknowledgement

Supported by : 국립해양생물자원관

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