Development of selective media for Enterococci

장구균 검출 배지 개발

  • Chang, Dong-Ho (Department of Biological Science and Biotechnology, Hannam University) ;
  • Yoon, Jun-Beom (Department of Biological Science and Biotechnology, Hannam University) ;
  • Lee, Keun Heon (Humas Co., Ltd.) ;
  • Park, Kyeong Ryang (Department of Biological Science and Biotechnology, Hannam University)
  • 장동호 (한남대학교 대덕밸리캠퍼스 생명시스템과학과) ;
  • 윤준범 (한남대학교 대덕밸리캠퍼스 생명시스템과학과) ;
  • 이근헌 (주)휴마스) ;
  • 박경량 (한남대학교 대덕밸리캠퍼스 생명시스템과학과)
  • Received : 2015.12.17
  • Accepted : 2016.01.15
  • Published : 2016.03.31


An enterococci selective medium was developed to detect the presence of enterococci for use as a fecal contamination indicator. Among several media which have been known to detect enterococci, the following 9 different kinds of media were selected: Enterococci Confirmatory agar, Azide dextrose agar, Bromocresol-purple azide agar, Esculin bile agar, Citrate azide tween carbonate agar, KF Streptococcus agar, BROLACIN agar, Kanamycin esculin azide agar, and Membrane filter Enterococcus selective agar. Various components from the nine media were mixed to develop a more effective enterococcus selective medium. The newly developed medium named as 'Enterococcus Mixed medium' was more effective than the previous 9 media. Enterococci strains (Enterococcus avium KACC 10788, Enterococcus faecium KACC 11954, Enterococcus saccharolyticus KACC 10783, Enterococcus durans KACC 10787, Enterococcus faecalis KACC 11304, and Enterococcus hirae KACC 10779) and non-enterococci strains (Escherichia coli KACC 10005, Staphylococcus aureus subsp. aureus KACC 10768, and Bacillus subtilis KACC 10111) were used to test the new medium. As a result, the enterococci strains grew well on the Enterococcus Mixed medium whereas the non-enterococci strains did not grow well on it. Additionally, growth of enterococci with freshwater and seawater samples was observed to be good on the Enterococcus Mixed medium. The result of this study confirmed that the Enterococcus Mixed medium was effective in detecting the target enterococci.


Enterococcus;freshwater;seawater;selective media


Supported by : 한남대학교


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